Failure to detect abnormal prion protein and scrapie-associated fibrils 6 wk after intracerebral inoculation of genetically susceptible sheep with scrapie agent

Canadian Journal of Veterinary Research

Volumn 66, Issue 4, 2002, Pages 289-294

  Hamir, Amir N ^a   Miller, Janice M ^a   Stack, Mick J ^a   Chaplin, Melanie J ^a  

^a NATIONAL ANIMAL DISEASE CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRION PROTEIN; PROTEINASE;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BRAIN INJURY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTRON MICROSCOPY; EUTHANASIA; FEMALE; GENETIC SUSCEPTIBILITY; HISTOPATHOLOGY; IMMUNOHISTOCHEMISTRY; INOCULATION; LAMB; MALE; NONHUMAN; PROTEIN ANALYSIS; SCRAPIE; SCRAPIE AGENT; SHEEP; WESTERN BLOTTING;

ANIMALS; ANIMALS, NEWBORN; BLOTTING, WESTERN; BRAIN; FEMALE; GENETIC PREDISPOSITION TO DISEASE; IMMUNOBLOTTING; INJECTIONS; MALE; PREDICTIVE VALUE OF TESTS; PRP 27-30 PROTEIN; PRPSC PROTEINS; SCRAPIE; SHEEP; TIME FACTORS;

ANIMALIA; OVIS ARIES; SCRAPIE PRION;

EID: 0036808534     PISSN: 08309000     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Hamir AN, Miller JM, Schmerr MJ, et al. Diagnosis of preclinical and subclinical scrapie in a naturally infected sheep flock utilizing currently available postmortem diagnostic techniques. J Vet Diagn Invest 2001;13:152-154.
2. Cutlip RC, Miller JM, Race RE, et al. Intracerebral transmission of scrapie to cattle. J Infect Dis 1994;169:814-820.
3. Cutlip RC, Miller JM, Lehmkuhl HD. Second passage of a US scrapie agent in cattle. J Comp Pathol 1997;117:271-275.
4. Hamir AN, Cutlip RC, Miller JM, et al. Preliminary findings on the experimental transmission of chronic wasting disease agent of mule deer to cattle. J Vet Diagn Invest 2001;13:91-96.
5. Detwiler LA. Scrapie. Rev Sci Tech Off Int Epizoot 1992;11: 491-537.
6. rPsc detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE). Acta Neuropathol 1999; 98:437-443.
7. Stack MJ, Keyes P, Scott AC. The diagnosis of bovine spongiform encephalopathy and scrapie by the detection of fibrils and the abnormal protein isoform. In: Baker HF, Ridley RM, eds. Methods in Molecular Medicine - Prion Diseases. Totowa, New Jersey: Humana Press, 1996:85-104.
8. Miller JM, Jenny AL, Taylor WD, et al. Detection of prion protein in formalin-fixed brain by hydrated autoclaving immimohistochemistry for diagnosis of scrapie in sheep. J Vet Diagn Invest 1994;6:366-368.
9. Nakaoke R, Sakaguchi S, Atarashi R, et al. Early appearance but lagged accumulation of detergent-insoluble prion protein in the brains of mice inoculated with a mouse-adapted Creutzfeldt-jakob disease agent. Cell Mol Neurobiol 2000; 20:717-330.