Expression profiling with oligonucleotide arrays: Technologies and applications for neurobiology

Neurochemical Research

Volumn 27, Issue 10, 2002, Pages 1005-1026

  Sendera, Timothy J ^a   Dorris, David ^a   Ramakrishnan, Ramesh ^a   Nguyen, Allen ^a   Trakas, Dionisios ^a   Mazumder, Abhijit ^a  

^a MOTOROLA INC   (United States)

Author keywords

DNA array; Expression ratio; Fabrication; Oligonucleotide; Reproducibility

Indexed keywords

ARTICLE; DNA FINGERPRINTING; DNA MICROARRAY; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE TARGETING; GENETIC ANALYSIS; GENOTYPE; HUMAN; HUMAN CELL; NEUROBIOLOGY; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; REPRODUCIBILITY; STATISTICAL ANALYSIS; TISSUE SECTION; UNINDEXED SEQUENCE;

BRAIN; GENE EXPRESSION PROFILING; HUMANS; NEUROBIOLOGY; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REPRODUCIBILITY OF RESULTS; RNA;

EID: 0036807321     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020948603490     Document Type: Article

References (79)

1. Brown, P. O. and Botstein, D. 1999. Exploring the new world of the genome with DNA microarrays. Nat. Genet. 21 (suppl. 1): 33-37.
2. Young, R. A. 2000. Biomedical discovery with DNA arrays. Cell 102:9-15.
3. Lockhart, D. J. and Winzeler, E. A. 2000. Genomics, gene expression, and DNA arrays. Nature 405:827-836.
4. Bassett, D. E., Jr., Eisen, M. B., and Boguski, M. S. 1999. Gene expression infomatics - It's all in your mine. Nat. Genet. 21 (suppl. 1):51-55.
5. Quackenbush, J. 2001. Computational analysis of microarray data. Nat. Rev. Genet. 2:418-427.
6. Heller, R., Schena, M., Chai, A., Shalon, D., Bedilion, T., Gilmore, J., Woolley, D. E., and Davis, R. W. 1997. Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proc. Natl. Acad. Sci. U.S.A. 94:2150-2155.
7. Marton, M. J., DeRisi, J. L., Bennett, H. A., Iyer, V. R., Meyer, M. R., Roberts, C., Stoughton, R., Burchard, J., Slade, D., Dai, H., Bassett, D. E., Jr., Hartwell, L. H., Brown, P. O., and Friend, S. H. 1998. Drug target validation and identification of secondary drug target effects using DNA microarrays. Nat. Med. 4: 1293-1301.
8. Huang, P., Feng, L., Oldham, E. A., Keating, M. J., and Plunkett, W. 2000. Superoxide dismutase as a target for the selective killing of cancer cells. Nature 407:390-395.
9. Lam, L. T., Pickeral, O. K., Peng, A. C., Rosenwald, A., Hurt, E. M., Giltnane, J. M., Averett, L. M., Zhao, H., Davis, R. E., Sathyamoorthy, M., Wahl, L. M., Harris, E. D., Mikovits, J. A., Monks, A. P., Hollingshead, M. G., Sausville, E. A., and Staudt, L. M. 2001. Genomic-scale measurement of mRNA turnover and the mechanisms of action of the anti-cancer drug flavopiridol. Genome Biol. 2:41.1-41.11.
10. Iyer, V. R., Eisen, M. B., Ross, D. T., Schuler, G., Moore, T., Lee, J. C. F., Trent, J. M., Staudt, L. M., Hudson, J. Jr., Boguski, M. S., Lashkari, D., Shalon, D., Botstein, D., and Brown, P.O. 1999. The transcriptional program in the response of human fibroblasts to serum. Science 283:83-87.
11. Perou, C. M., Sorlie, T., Eisen, M. B., van de Rijn, M., Jeffrey, S. S., Rees, C. A., Pollack, J. R., Ross, D. T., Johnsen, H., Akslen, L. A., Fluge, O., Pergamenschikov, A., Williams. C., Zhu, S. X., Lonning, P. E., Borresen-Dale, A.-L., Brown, P. O., and Botstein, D. 2000. Molecular portraits of human breast tumors. Nature 406:747-752.
12. Bittner, M., Meltzer, P., Chen, Y., Jiang, Y., Seftor, E., Hendrix, M., Radmacher, M., Simon, R., Yakhini, Z., Ben-Dor, A., Sampas, N., Dougherty, E., Wang, E., Marincola, F., Gooden, C., Lueders, J., Glatfelter, A., Pollock, P., Carpten, J., Gillanders, E., Leja, D., Dietrich, K., Beaudry, C., Berens, M., Alberts, D., Sondak, V., Hayward, N., and Trent, J. 2000. Molecular classification of cutaneous malignant melanoma by gene expression profiling. Nature 406:536-540.
13. Shoemaker, D. D., Schadt, E. E., Armour, C. D., He, Y.D., Garrett-Engele, P., McDonagh, P. D., Loerch, P. M., Leonardson, A., Lum, P. Y., Cavet, G., Wu, L. F., Altschuler, S. J., Edwards, S., King, J., Tsang, J. S., Schimmack, G., Schelter, J. M., Koch, J., Ziman, M., Marton, M. J., Li, B., Cundiff, P., Ward, T., Castle, J., Krolewski, M., Meyer, M. R., Mao, M., Burchard, J., Kidd, M. J., Dai, H., Phillips, J. W., Linsley, P. S., Stoughton, R., Scherer, S., and Boguski, M. S. 2001. Experimental annotation of the human genome using microarray technology. Nature 409:922-927.
14. Scherf, U., Ross, D. T., Waltham, M., Smith, L. H., Lee, J. K., Tanabe, L., Kohn, K. W., Reinhold, W. C., Myers, T. G., Andrews, D. T., Scudiero, D. A., Eisen, M. B., Sausville, E. A., Pommier, Y., Botstein, D., Brown, P. O., and Weinstein, J. N. 2000. A gene expression database for the molecular pharmacology of cancer. Nat. Genet. 24:236-244.
15. Staunton, J. E., Slonim, D. K., Coller, H. A., Tamayo, P., Angelo, M. J., Park, J., Scherf, U., Lee, J. K., Reinhold, W. O., Weinstein, J. N., Mesirov, J. P., Lander, E. S., and Golub. T. R. 2001. Chemosensitivity prediction by transcriptional profiling. Proc. Natl. Acad. Sci. U.S.A. 98:10787-10792.
16. Alizedah, A. A., Eisen, M. B., Davis, R. E., Ma, C., Lossos, I. S., Rosenwald, A., Boldrick, J. C., Sabet, H., Tran, T., Yu, X., Powell, J. I., Yang, L., Marti, G. E., Moore, T., Hudson, J., Jr., Lu, L., Lewis, D. B., Tibshirani, R., Sherlock, G., Chan, W. C., Greiner, T. C., Weisenburger, D. D., Armitage, J. O., Warnke, R., Levy, R., Wilson, W., Grever, M. R., Byrd, J. C., Botstein, D., Brown, P. O., and Staudt, L. M. 2000. Distinct types of B-cell lymphoma identified by gene expression profiling. Nature 403:503-511.
17. Golub, T. R., Slonim, D. K., Tamayo, P., Huard, C., Gaasenbeek, M., Mesirov, J. P., Coller, H., Loh, M. L., Downing, J. R., Caligiuri, M. A., Bloomfield, C. D., and Lander, E. S. 1999. Molecular classification of cancer: Class discovery and class prediction by gene expression monitoring. Science 286:531-537.
18. Unger, M. A., Rishi, M., Clemmer, V. B., Hartman, J. L., Keiper, E. A., Greshock, J. D., Chodosh, L. A., Liebman, M. N., and Weber, B. L. 2001. Characterization of adjacent breast tumors using oligonucleotide microarrays. Breast Cancer Res. 3:336-341.
19. Bhattacharjee, A., Richards, W. G., Staunton, J., Li, C., Monti, S., Vasa, P., Ladd, C., Beheshti, J., Bueno, R., Gillette, M., Loda, M., Weber, G., Mark, E. J., Lander, E. S., Wong, W., Johnson, B. E., Golub, T. R., Sugarbaker, D. J., and Meyerson, M. 2001. Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses. Proc. Natl. Acad. Sci. U.S.A. 98:13790-13795.
20. Garber, M. E., Troyanskaya, O. G., Schluens, K., Petersen, S., Thaesler, Z., Pacyna-Gengelbach, M., van de Rijn, M., Rosen, G. D., Perou, C. M., Whyte, R. I., Altman, R. B., Brown, P. O., Bolstein D., and Petersen, I. 2001. Diversity of gene expression in adenocarcinoma of the lung. Proc. Natl. Acad. Sci. U.S.A. 98: 13784-13789.
21. Pomeroy, S. L., Tamayo, P., Gaasenbeek, M., Sturla, L. M., Angelo, M., McLaughlin, M. E., Kim, J. Y., Goumnerova, L. C., Black, P. M., Lau, C., Allen, J. C., Zagzag, D., Olson, J. M., Curran, T., Wetmore, C., Biegel, J. A., Poggio, T., Mukherjee, S., Rifkin, R., Califano, A., Stolovitzky, G., Louis, D. N., Mesirov, J. P., Lander, E. S., and Golub, T. R. 2002. Prediction of central nervous system embryonal tumour outcome based on gene expression. Nature 415:436-442.
22. van't Veer, L. J., Dai, H., van de Vijver, M. J., He, Y. D., Hart, A. A. M., Mao, M., Peterse, H. L., van der Kooy, K., Marton, M. J., Witteveen, A.T., Schreiber, G.J., Kerkhoven, R. M., Roberts, C., Linsley, P. S., Bernards, R., and Friend, S. H. 2002. Gene expression profiling predicts clinical outcome of breast cancer. Nature 415:530-536.
23. McCarthy, J. J. and Hilfiker, R. 2000. The use of single nucleotide polymorphism maps in pharmacogenomics. Nat. Biotechnol. 18:505-508.
24. Sandberg, R., Yasuda, R., Pankratz, D. G., Carter, T. A., Del Rio, J. A., Wodicka, L., Mayford, M., Lockhart, D. J., and Barlow, C. 2000. Regional and strain-specific gene expression mapping in the adult mouse brain. Proc. Natl. Acad. Sci. U S A 97:11038-11043.
25. Zirlinger, M., Kreiman, G., and Anderson, D. J. 2001. Amygdala-enriched genes identified by microarray technology are restricted to specific amygdaloid subnuclei. Proc. Natl. Acad. Sci. U S A 98:5270-5275.
26. Mody, M., Cao, Y., Cui, Z., Tay, K. Y., Shyong, A., Shimizu, E., Pham, K., Schultz, P., Welsh, D., and Tsien, J. Z. 2001. Genome-wide gene expression profiles of the developing mouse hippocampus. Proc. Natl. Acad. Sci. U S A 98:8862-8867.
27. Konu, O., Kane, J. K., Barrett, T., Vawter, M. P., Chang, R., Ma, J. Z., Donovan, D. M., Sharp, B., Becker, K. G., and Li, M. D. 2001. Region-specific transcriptional response to chronic nicotine in rat brain. Brain Res. 909:194-203.
28. Chabas, D., Baranzini, S. E., Mitchell, D., Bernard, C. C., Rittling, S. R., Denhardt, D. T., Sobel, R. A., Lock, C., Karpuj, M., Pedotti, R., Heller, R., Oksenberg, J. R., and Steinman, L. 2001. The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease. Science 294: 1731-1735.
29. Jin, K., Mao, X. O., Eshoo, M. W., Nagayama, T., Minami, M., Simon, R. P., and Greenberg, D. A. 2001. Microarray analysis of hippocampal gene expression in global cerebral ischemia. Ann Neurol. 50:93-103.
30. Hakak, Y., Walker, J. R., Li, C., Wong, W. H., Davis, K. L., Buxbaum, J. D., Haroutunian, V., and Fienberg, A. A. 2001. Genome-wide expression analysis reveals dysregulation of myelination-related genes in chronic schizophrenia. Proc. Natl. Acad. Sci. U.S.A. 98:4746-4751.
31. Mirnics, K., Middleton, F. A., Marquez, A., Lewis, D. A., and Levitt, P. 2000. Molecular characterization of schizophrenia viewed by microarray analysis of gene expression in prefrontal cortex. Neuron 28:53-67.
32. Pasinetti, G. M. 2001. Use of cDNA microarray in the search for molecular markers involved in the onset of Alzheimer's disease dementia. J. Neurosci. Res. 65:471-476.
33. Brown, V. M., Ossadtchi, A., Khan, A. H., Cherry, S. R., Leahy, R. M., and Smith, D. J. 2002. High-throughput imaging of brain gene expression. Genome Res. 12:244-254.
34. Lipshutz, R. J., Fodor, S. P. A., Gingeras, T. R., and Lockhart, D. J. 1999. High density synthetic oligonucleotide arrays. Nat. Genet. 21(suppl 1):20-24.
35. Hughes, T. R., Mao, M., Jones, A. R., Burchard, J., Marton, M. J., Shannon, K. W., Lefkowitz, S. M., Ziman, M., Schelter, J. M., Meyer, M. R., Kobayashi, S., Davis, C., Dai, H., He, Y. D., Stephaniants, S. B., Cavet, G., Walker, W. L., West, A., Coffey, E., Shoemaker, D. D., Stoughton, R., Blanchard, A. P., Friend, S. H., and Linsley, P. S. 2001. Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer. Nat. Biotech. 19:342-347.
36. Kane, M. D., Jatkoe, T. A., Stumpf, C. R., Lu, J., Thomas, J. D., and Madore, S. J. 2000. Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays. Nucleic Acids Res. 28:4552-4557.
37. Hu, G. K., Madore, S. J., Moldover, B., Jatkoe, T., Balaban, D., Thomas, J., and Wang, Y. 2001. Predicting splice variant from DNA chip expression data. Genome Res. 11:1237-1245.
38. Wodicka, L., Dong, H., Mittmann, M., Ho, M.-H., and Lockhart, D. J. 1997. Genome-wide expression monitoring in Saccharomyces cerevisiae. Nat. Biotechnol. 15:1359-1367.
39. Hill, A. A., Hunter, C. P., Tsung, B. T., Tucker-Kellogg, and Brown, E. L. 2000. Genomic analysis of gene expression in C. elegans. Science 290:809-812.
40. McDonald, M. J. and Rosbash, M. 2001. Microarray analysis and organization of circadian gene expression in Drosophila. Cell 107:567-578.
41. Irving, P., Troxler, L., Heuer. T. S., Belvin, M., Kopczynski, C., Reichhart, J. M., Hoffmann, J. A., and Hetru, C. 2001. A genome-wide analysis of immune responses in Drosophila. Proc. Natl. Acad. Sci. USA 98:15119-15124.
42. Selinger, D. W., Cheung, K. J., Mei, R., Johansson, E. M., Richmond, C. S., Blattner, F. R., Lockhart, D. J., and Church, G. M. 2000. RNA expression analysis using a 30 base pair resolution Escherichia coli genome array. Nat. Biotechnol. 18: 1262-1268.
43. Baugh, L. R., Hill, A. A., Brown, E. L., and Hunter, C. P. 2001. Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res. 29:e29.
44. Fodor, S. P. A., Read, J. L., Pirrung, M. C., Stryer, L., Lu, A. T., and Solas, D. 1991. Light-directed, spatially addressable parallel chemical synthesis. Science 251:767-773.
45. Maskos, U. and Southern, E. M. 1992. Oligonucleotide hybridizations on glass supports: A novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesized in situ. Nucleic Acids Res. 20:1679-1684.
46. Nanthakumar, A., Pon, R. T., Mazumder, A., Yu, S., and Watson, A. 2000. Solid-phase oligonucleotide synthesis and flow cytometric analysis with microspheres encoded with covalently attached fluorophores. Bioconj. Chem. 11:282-288.
47. Singh-Gasson, S., Green, R. D., Yue, Y., Nelson, C., Blattner, F., Sussman, M. R., and Cerrina, F. 1999. Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array. Nat. Biotechnol. 17:974-978.
48. Gao, X., LeProust, E., Zhang, H., Srivannavit, O., Gulari, E., Yu, P., Nishiguchi, C., Xiang, Q., and Zhou, X. 2001. A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids. Nucleic Acids Res. 29:4744-4750.
49. Butler, J. H., Cronin, M., Anderson, K. M., Biddison, G. M., Chatelain, F., Cummer, M., Davi, D. J., Fisher, L., Frauendorf, A. W., Frueh, F. W., Gjerstad, C., Harper, T. F., Kernahan, S. D., Long, D. Q., Pho, M., Walker, J. A., 2nd and Brennan, T. M. 2001. In situ synthesis of oligonucleotide arrays by using surface tension. J. Am. Chem. Soc. 123:8887-8894.
50. Elghanian, R., Xu, Y., McGowen, J., Siethoff, M., Liu, C. G., Winick, J., Fuller, N., Ramakrishnan, R., Beuhler, A., Johnson, T., Mazumder, A., and Brush, C. 2001. The use and evaluation of 2+2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix. Nucleosides Nucleotides Nucleic Acids 20:1371-1375.
51. Ramakrishnan, R., Dorris, D., Lublinsky, A., Nguyen, A., Domanus, M., Prokhorova, A., Gieser, L., Touma, E., Lockner, R., Tata, M., Zhu, X., Patterson, M., Shippy, R., Sendera, T., and Mazumder, A. 2002. An assessment of Motorola Codelink™ microarray performance for gene expression profiling applications. Nucleic Acids Res. 30:in press.
52. Kwiatkowski, M., Fredriksson, S., Isaksson, A., Nilsson, M., and Landegren, U. 1999. Inversion of in situ synthesized oligonucleotides: Improved reagents for hybridization and primer extension in DNA microarrays. Nucleic Acids Res. 27:4710-4714.
53. Chrisey, L. A., O'Ferall, C. E., Spargo, B. J., Dulcey, C. S., and Calvert, J. M. 1996. Fabrication of patterned DNA surfaces. Nucleic Acids Res. 24:3040-3047.
54. Beier, M. and Hoheisel, J. D. 2000. Production by quantitative photolithographic synthesis of individually quality checked DNA microarrays. Nucleic Acids Res, 28:e11.
55. Lindroos, K., Liljedahl, U., Raitio, M., and Syvanen, A.-C, 2001. Minisequencing on oligonucleotide microarrays: Comparison of immobilization chemistries. Nucleic Acids Res. 29:e69.
56. Lemeshko, S. V., Powdrill, T., Belosludtsev, Y. Y., and Hogan, M. 2001. Oligonucleotides form a duplex with non-helical properties on a positively charged surface. Nucleic Acids Res. 29: 3051-3058.
57. Chee, M., Yang, R., Hubbell, E., Berno, A., Huang, X. C., Stern, D., Winkler, J., Lockhart, D. J., Morris, M. S., and Fodor, S. P. 1991. Accessing genetic information with high-density DNA arrays. Science 274:610-614.
58. Yang, L., Tran, D. K., and Wang, X. 2001. BADGE, Beads Array for the Detection of Gene Expression, a high-throughput diagnostic bioassay. Genome Res. 11:1888-1898.
59. Kononen, J., Bubendorf, L., Kallioniemi, A., Barlund, M., Schraml, P., Leighton, S., Torhorst, J., Mihatsch, M. J., Sauter, G., and Kallioniemi, O. P. 1998. Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 4:844-847.
60. Yan, H., Dobbie, Z., Gruber, S. B., Markowitz, S., Romans, K., Giardiello, F. M., Kinzler, K. W. and Vogelstein, B. 2002. Small changes in expression affect predisposition to tumorigenesis. Nat. Genet. 30:25-26.
61. Jin, W., Riley, R. M., Wolfinger, R. D., White, K. P., Passador-Gurgel, G., and Gibson, G. 2001. The contributions of sex, genotype and age to transcriptional variance in Drosophila melanogaster. Nat. Genet. 29:389-395.
62. Chaudhari, N. and Hahn, W. E. 1983. Genetic expression in the developing brain. Science 220:924-928.
63. Pritchard, C. C., Hsu, L., Delrow, J., and Nelson, P. S. 2001. Project normal: Defining normal variance in mouse gene expression. Proc. Natl. Acad. Sci. U S A 98:13266-13271.
64. Lee, P. D., Sladek, R., Greenwood, C. M., and Hudson, T. J. 2002. Control genes and variability: Absence of ubiquitous reference transcripts in diverse mammalian expression studies. Genome Res. 12:292-297.
65. Yue, H., Eastman, P. S., Wang, B. B., Minor, J., Doctolero, M. H., Nuttall, R. L., Stack, R., Becker, J. W., Montgomery, J. R., Vainer, M., and Johnston, R. 2001. An evaluation of the performance of cDNA microarrays for detecting changes in global gene expression. Nuc. Acids Res. 29:e41.
66. Lee, M.-L. T., Kuo, F. C., Whitmore, G. A., and Sklar, J. 2000. Importance of replication in microarray gene expression studies: Statistical methods and evidence from repetitive cDNA hybridizations. Proc. Natl. Acad. Sci. U S A 97:9834-9839.
67. Simpson, R. J., Jr., Johnson, T. A., and Amara, I. A. 1988. The box-plot: An exploratory analysis graph for biomedical publications. Am. Heart J. 116:1663-1665.
68. Williamson, D. F., Parker, R. A., and Kendrick, J. S. 1989. The box plot: A simple visual method to interpret data. Ann. Intern. Med. 110:916-921.
69. Kerr, M. K. and Churchill, G. A. 2001. Statistical design and the analysis of gene expression microarray data. Genet. Res. 77: 123-128.
70. Jia, M. H., Larossa, R. A., Lee, J. M., Rafalski, A., Derose, E., Gonye, G., and Xue, Z. 2000. Global expression profiling of yeast treated with an inhibitor of amino acid biosynthesis, sulfometuron methyl. Physiol. Genomics 3:83-92.
71. Arfin, S. M., Long, A. D., Ito, E. T., Tolleri, L., Riehle, M. M., Paegle, E. S., and Hatfield, G. W. 2000. Global gene expression profiling in Escherichia coli K12. The effects of integration host factor. J. Biol. Chem. 275:29672-29684.
72. Chow, N., Cox, C., Callahan, L. M., Weimer, J. M., Guo, L., and Coleman, P. D. 1998. Expression profiles of multiple genes in single neurons of Alzheimer's disease, Proc. Natl. Acad. Sci. U S A 95:9620-9625.
73. Canete-Soler, R., Wu, J., Zhai, J., Shamim, M., and Schlaepfer, W, W. 2001. p190RhoGEF Binds to a destabilizing element in the 3' untranslated region of light neurofilament subunit mRNA and alters the stability of the transcript. J. Biol. Chem. 276: 32046-32050.
74. Alvarez-Bolado, G., Rodriguez-Sanchez, P., Tejero-Diez, P., Fairen, A., and Diez-Guerra, F. J. 1996. Neurogranin in the development of the rat telencephalon. Neuroscience 73:565-580.
75. Elson, G. C., de Coignac, A. B., Aubry, J. P., Delneste, Y., Magistrelli, G., Holzwarth, J., Bonnefoy, J. Y., and Gauchat, J. F. 1999. BSMAP, a novel protein expressed specifically in the brain whose gene is localized on chromosome 19p12. Biochem. Biophys. Res. Commun. 264:55-62.
76. Bellocchio, E. E., Reimer, R. J., Fremeau, R. T., Jr., and Edwards. R. H. 2000 Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter. Science 289:957-960.
77. Liang, R., Limesand, S. W., and Anthony, R. V. 1999. Structure and transcriptional regulation of the ovine placental lactogen gene. Eur. J. Biochem. 265:883-895.
78. Ginsberg, S. D., Crino, P. B., Hemby, S. E., Weingarten, J. A., Lee, V. M., Eberwine, J. H., and Trojanowski, J. Q. 1999. Predominance of neuronal mRNA in individual Alzheimer's disease senile plaques. Ann. Neurol. 45:174-181.
79. Ginsberg, S. D., Hemby, S. E., Lee, V. M., Eberwine, J. H., and Trojanowski, J. Q. 2000. Expression profile of transcripts in Alzheimer's disease tangle-bearing CA1 neurons. Ann. Neurol. 48:77-87.