Characterization of alkaline thermoactive cellulase-free xylanases from alkalophilic Bacillus (NCL 87-6-10)

Journal of Biochemistry, Molecular Biology and Biophysics

Volumn 6, Issue 5, 2002, Pages 325-334

  Balakrishnan, H ^a   Kamal Kumar, B ^a   Dutta Choudhury, M ^a   Rele, M V ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Alkaline xylanase; Alkalophilic Bacillus sp; Cellulase free xylanase; Culture filtrates

Indexed keywords

ALKALINE XYLAN ENDO 1,3 BETA XYLOSIDASE; CATION; CELLULASE; DODECYL SULFATE SODIUM; MONOMER; OLIGOSACCHARIDE; POLYACRYLAMIDE GEL; PROTEIN; SEPHAROSE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOBIOSE; XYLOSE; XYLOTRIOS; ISOENZYME; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; BACILLUS; BACTERIAL GROWTH; CHEMICAL COMPOSITION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME KINETICS; ENZYME PURIFICATION; GEL ELECTROPHORESIS; HOMOGENATE; HYDROLYSIS; INCUBATION TIME; MOLECULAR WEIGHT; NONHUMAN; PH; SEPARATION TECHNIQUE; TEMPERATURE; WHEAT BRAN; YEAST; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACILLUS SP.; BACTERIA (MICROORGANISMS); TRITICUM AESTIVUM;

BACILLUS; CELLULASE; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ISOENZYMES; KINETICS; MOLECULAR WEIGHT; SUBSTRATE SPECIFICITY; XYLAN ENDO-1,3-BETA-XYLOSIDASE; XYLOSIDASES;

EID: 0036805879     PISSN: 10258140     EISSN: None     Source Type: Journal    
DOI: 10.1080/1025814021000003229     Document Type: Article

References (22)

1. Viikari, L., Kantelinen, A., Sundquist, J. and Linko, M. (1994) "Xylanases in bleaching: from an idea to industry", FEMS Microbiol. Rev. 13, 335-350.
2. Srinivasan, M.C. and Rele, M.V. (1995) "Cellulase-free xylanases from microorganisms and their application to pulp and paper biotechnology: an overview", Indian J. Microbiol. 35(2), 93-101.
3. Nakamura, S., Wakabayashi, K., Nakai, R. and Horikoshi, K. (1993) "Production of alkaline xylanase by a newly isolated alkalophilic Bacillus strain 41 M-1", World J. Microbiol. Biotechnol. 9, 221-224.
4. Bansod, S.M. and Rele, M.V. (1998) "Alkaline xylanase from an alkalotolerant Cephalosporium sp.: characteristics", J. Biochem. Mol. Biol. Biophys. 2, 121-128.
5. Balakrishnan, H., Dutta-Choudhry, M., Srinivasan, M.C. and Rele, M.V. (1992) "Cellulase-free xylanase production from an alkalophilic Bacillus species", World J. Microbiol. Biotechnol. 8, 627-631.
6. Sedmek, J.J. and Grossberg, S.E. (1977) "A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G250", Anal. Biochem. 79, 544-552.
7. Berghem, L.E.R. and Petterson, L.G. (1973) "The mechanism of enzymatic cellulose degradation of a cellulolytic enzyme from Trichoderma viride active on highly ordered cellulose", Eur. J. Biochem. 37, 21-30.
8. Reisfeld, R.A., Lewis, J. and Williams, D.E. (1962) "Disk electrophoresis of basic proteins and peptides on polyacrylamide gels", Nature (London) 195, 281-283.
9. Laemmli, U.K. (1970) "Cleavage of structural proteins during assembly of head of bacteriophage T4", Nature (London) 227, 680-685.
10. Ghadge, G.D., Bodhe, A.M., Dhume, S.T., Rele, M.V. and Vartak, H.G. (1984) "A versatile gel casting cum electrophoresis apparatus", J. Biosci. 6(1), 135-145.
11. Davis, B.J. (1964) "Disc electrophoresis-II. Method and application to human serum proteins", Ann. N.Y. Acad. Sci. 121, 404-427.
12. Blakesley, R.W. and Boezi, J.A. (1977) "A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250", Anal. Biochem. 82, 580-582.
13. Chinnathambi, S., Lachke, A. and Radhakrishnan, S. (1995) "Purification of α-L-arabinofuranosidase using a single column miniscale isoelectric focussing unit", J. Chromatogr. A 705, 400-402.
14. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) "Colorimetric method for determination of sugars and related substances", Anal. Chem. 28, 350-356.
15. Balakrishnan, H. (1993) Studies on Microbial Xylanase, Ph.D. Thesis, University of Poona (Pune, Maharashtra, India).
16. Kamal Kumar, B. (2001) Characterization of alkaline xylanase from an alkalophilic Bacillus and its application in detergents, M.Sc. Thesis, Bharathidasan University (Tiruchirappalli, Tamilnadu, India).
17. Wong, K.K.Y., Tan, L.U.L. and Saddler, J.N. (1988) "Multiplicity of β-1, 4-xylanase in microorganisms: functions and applications", Microbiol. Rev. 52, 305-317.
18. Grabski, A.C. and Jeffries, T.W. (1991) "Production, purification and characterization of β-(1,4)-endoxylanase of Streptomyces roseiscleroticus", Appl. Environ. Microbiol. 57(4), 987-992.
19. Grabski, A.C., Forrester, I.T., Patel, R. and Jeffries, T.W. (1993) "Characterization and N-terminal amino acid sequences of β-(1,4) Endoxylanases from Streptomyces roseiscleroticus: purification incorporating a bioprocessing agent", Protein Expr. Purif. 4, 120-129.
20. Holden, F.R. and Walton, J.D. (1992) "Xylanases from the fungal maize pathogen Cochliobolus carbonum", Physiol. Mol. Plant Pathol. 40, 39-47.
21. Richards, G.N. and Shambe, T. (1976) "Production and purification of two hemicellulases from Cephalosporium sacchari", Carbohydr. Res. 49, 371-381.
22. Viikari, L., Kantelinen, A., Buchert, J. and Puls, J. (1994) "Enzymatic accessibility of xylans in lignocellulosic materials", Appl. Microbiol. Biotechnol. 41, 124-129.