Analysis of the microbial community structure and function of a laboratory scale enhanced biological phosphorus removal reactor

Environmental Microbiology

Volumn 4, Issue 10, 2002, Pages 559-569

  Levantesi, Caterina ^a   Serafim, Luísa S ^b   Crocetti, Gregory R ^c   Lemos, Paulo C ^b   Rossetti, Simona ^a   Blackall, Linda L ^c   Reis, Maria A M ^b   Tandoi, Valter ^a  

^a WATER RESEARCH INSTITUTE   (Italy)

^b DEPARTAMENTO DE QUÍMICA   (Portugal)

^c UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA, ANAEROBIC; BIODEGRADATION, ENVIRONMENTAL; GLYCOGEN; PHOSPHORUS; REFUSE DISPOSAL; SEWAGE;

ALPHAPROTEOBACTERIA; AMARICOCCUS; BACTERIA (MICROORGANISMS); GAMMAPROTEOBACTERIA;

EID: 0036802414     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.2002.00339.x     Document Type: Article

References (47)

1. Amann, R.I., Binder, B.J., Olson, R.J., Chisholm, S.W., Devereux, R., and Stahl, D.A. (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56: 1919-1925.
2. APHA, American Public Health Association (1998) Standard methods for the examination of water and wastewater. Washington, D.C.: APHA-AWWA-WEF.
3. Blackall, L.L. (1999) Bulking. In The Microbiology of Activated Sludge. Seviour, R.J., and Blackall, L.L. (eds). London: Kluwer Academic Publishers, pp. 147-160.
4. Bond, P.L., Erhart, R., Wagner, M., Keller, J., and Blackall, L.L. (1999a) Identification of some of the major groups of bacteria in efficient and non efficient biological phosphorus removal activated sludge systems. Appl Environ Microbiol 65: 4077-4084.
5. Bond, P.L., Keller, J., and Blackall, L.L. (1999b) Bio-P and non-bio-P bacteria identification by a novel microbial approach. Wat Sci Tech 39 (6): 13-20.
6. Bossier, P., and Verstraete, W. (1996) Triggers for microbial aggregation in activated sludge. Appl Microbiol Biotech 45: 1-6.
7. Bouchez, T., Patureau, D., Dabert, P., Juretschko, S., Dore, J., Delgenes, P. et al. (2000) Ecological study of a bioaugmentation failure. Environ Microbiol 2: 179-190.
8. Cech, J.S., and Hartman, P. (1993) Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Wat Res 27: 1219-1225.
9. Crocetti, G.R., Hugenholtz, P., Bond, P.L., Schuler, A., Keller, J., Jenkins, D., and Blackall, L.L. (2000) Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl Environ Microbiol 66: 1175-1182.
10. Crocetti, G.R., Banfield, J.F., Keller, J., Bond, P.L., and Blackall, L.L. (2002) Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater treatment processes. Microbiology (in press).
11. Dabert, P., Sialve, B., Delgenès, J.-P., Moletta, R., and Godon, J.-J. (2001) Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration. Appl Microbiol Biotech 55: 500-509.
12. Daims, H., Bruhl, A., Amann, R., Schleifer, K.H., and Wagner, M. (1999) The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: Development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 22: 434-444.
13. Eikelboom, D.H., and van Buijsen, H.J.J. (1983) Microscopic Sludge Investigation Manual, 2nd edn. Delft: TNO Research Institute of Environmental Hygiene.
14. Falvo, A., Levantesi, C., Rossetti, S., Seviour, R.J., and Tandoi, V. (2001) Synthesis of intracellular storage polymers by Amaricoccus kaplicensis, a tetrad forming bacterium present in activated sludge. J Appl Microbiol 91: 299-305.
15. Filipe, C.D.M., Daigger, G.T., and Grady, C.P.L. (2001a) Effects of pH on the rates of aerobic metabolism of phosphate-accumulating and glycogen-accumulating organisms. Water Environ Res 73: 213-222.
16. Filipe, C.D.M Daigger, G.T., and Grady, C.P.L. (2001b) pH as a key factor in the competition between glycogen-accumulating organisms and phosphorus-accumulating organisms. Water Environ Res 73: 223-232.
17. Fuhs, G.W., and Chen, M. (1975) Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microbial Ecol 2: 119-138.
18. Fukase, T., Shibata, M., and Miyaji, Y. (1985) The role of an anaerobic stage on biological phosphorus removal. Wat Sci Tech 17 (2-3): 69-80.
19. Hesselmann, R.P.X., Werlen, C., Hahn, D., van der Meer, J.R., and Zehnder, A.J.B. (1999) Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge. Syst Appl Microbiol 22: 454-465.
20. IWA-ASPD, C. (2001) Proceedings of the Conference on Microorganisms in Activated Sludge and Biofilm Processes. Rome: IWA
21. Jenkins, D., Richard, M.G., and Daigger, G.T. (1993) Manual on the Causes and Control of Activated Sludge Bulking and Foaming. New York: Lewis Publishers.
22. Jeon, C.O., Lee, D.S., Lee, M.W., and Park, J.M. (2001) Enhanced biological phosphorus removal in an anaerobic-aerobic sequencing batch reactor: Effect of pH. Water Environ Res 73: 301-306.
23. Lemos, P.C., Viana, C., Salgueiro, E.N., Ramos, A.M., Crespo, J.P.S.G, and Reis, M.A.M. (1998) Effect of carbon source on the formation of polyhydroxyalkanoates (PHA) by a phosphate-accumulating mixed culture. Enzyme Microbial Technol 22: 662-671.
24. Li, D.H., and Ganczarczyk, J.J. (1990) Structure of activated sludge flocs. Biotechnol Bioengineering 35: 57-65.
25. Liu, W.-T., Mino, T., Matsuo, T., and Nakamura, K. (1996) Biological phosphorus removal processes - effect of pH on anaerobic substrate metabolism. Wat Sci Tech 30 (1-2): 25-30.
26. Liu, W.-T., Nakamura, K., Matsuo, T., and Mino, T. (1997) Internal energy-based competition between polyphosphate- and glycogen-accumulating bacteria in biological phosphorus removal reactors-effect of P/C feeding ratio. Wat Res 31: 1430-1438.
27. Liu, W.-T., Nielsen, A.T., Wu, J.-H., Tsai, C.-S., Matsuo, Y., and Molin, S. (2001) In situ identification of polyphosphate-and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process. Environ Microbiol 3: 110-122.
28. Manz, W., Amann, R., Ludwig, W., Wagner, M., and Schleifer, K.-H. (1992) Phylogenetic oligonucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst Appl Microbiol 15: 593-600.
29. Maszenan, A.M., Seviour, R.J., Patel, B.K.C., Schumann, P., Burghardt, J., Tokiwa, Y., and Stratton, H.M (2000a) Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus. Tetrasphaera gen. nov. and description of two new species, Tetrasphaera japonica sp. nov. and Tetrasphaera australiensis sp. nov. Int J Syst Evol Microbiology 50: 593-603.
30. Maszenan, A.M., Seviour, R.J., Patel, B.K.C., and Wanner, J. (2000b) A fluorescently-labelled rRNA targeted oligonucleotide probe for the in situ detection of G-bacteria of the genus Amaricoccus activated sludge. J Appl Microbiol 88: 826-835.
31. Mino, T., Arun, V., Tsuzuki, Y., and Matsuo, T. (1987) Effect of phosphorus accumulation on acetate metabolism in the biological phosphorus removal process. In Biological Phosphate Removal from Wastewaters. Ramadori, R. (eds). Oxford: Pergamon Press, pp. 27-38.
32. Murray, R.G.E., Doetsch, R.N., and Robinow, C.F. (1994) Determinative and cytological microscopy. In Methods for General and Molecular Bacteriology. Gerhardt, P., Murray, R.G.E., Wood, W.A., and Krieg, N.R. (eds). Washington, D.C.: American Society for Microbiology, pp. 21-36.
33. Nakamura, K., Hiraishi, A., Yoshimi, Y., Kawaharasaki, M., Masuda, K., and Kamagata, Y. (1995) Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Bacteriol 45: 17-22.
34. Nielsen, A.T., Liu, W.-T., Filipe, C., Grady, L., Molin, S., and Stahl, D.A. (1999) Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor. Appl Environ Microbiol 65: 1251-1258.
35. Ostle, A.G., and Holt, J.G. (1982) Nile Blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 44: 238-241.
36. 13C-NMR labelling experiments. Wat Res 30: 2128-2138.
37. Pramanik, J., Trelstad, P.L., Schuler, A.J., Jenkins, D., and Keasling, J.D. (1999) Development and validation of a flux-based stoichiometric model for enhanced biological phosphorus removal metabolism. Wat Res 33: 462-476.
38. Rees, G.N., Vasiliadis, G., May, J.W., and Bayly, R.C. (1992) Differentiation of polyphosphate and poly-β-hydroxybutyrate granules in an Acinetobacter sp. isolated from activated sludge. FEMS Microbiol Lett 94: 171-174.
39. Satoh, H., Mino, T., and Matsuo, T. (1992) Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes. Wat Sci Tech 26: 933-942.
40. Satoh, H., Mino, T., and Matsuo, T. (1994) Deterioration of enhanced biological phosphorus removal by the domination of microorganisms without polyphosphate accumulation. Wat Sci Tech 30: 203-211.
41. Schuler, A.J., and Jenkins, D. (2001) Effects of Ph on Enhanced Biological Phosphorus Removal Metabolisms. 2nd IWA World Water Congress. Berlin, Germany: IWA, pp. 75-81.
42. Smolders, G.J.F. (1995) A metabolic model of the biological phosphorus removal stoichiometry, kinetics and dynamic behaviour. The Netherlands: University of Technology.
43. Smolders, G.J.F., van der Meij, J., van Loosdrecht, M.C.M., and Heijnen, J.J. (1994a) Model of the anaerobic metabolism of the biological phosphorus removal process - stoichiometry and pH influence. Biotechnol Bioengineering 43: 461-470.
44. Smolders, G.J.F., van der Meij, J., van Loosdrecht, M.C.M., and Heijnen, J.J. (1994b) Stoichiometric model of the aerobic metabolism of the biological phosphorus removal process. Biotechnol Bioengineering 44: 837-848.
45. Stante, L., Cellamare, C.M., Malaspina, F., Bortone, G., and Tilche, A. (1997) Biological phosphorus removal by pure culture of Lampropedia spp. Wat Res 31: 1317-1324.
46. Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D., and Schleifer, K.-H. (1994) Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl Environ Microbiol 60: 792-800.
47. Wentzel, M.C., Loewenthal, R.E., Ekama, G.A., and Marais, G.V. (1988) Enhanced polyphosphate organism cultures in activated sludge systems - Part 1: Enhanced culture development. Water SA 14: 81-92.