Colorimetric quantitation of in vitro cell density using carmine, a chromosome-specific stain

Toxicology in Vitro

Volumn 16, Issue 5, 2002, Pages 573-579

  Garcia Gasca T ^a   Paz Gonzalez V ^a   Moncada Alvarez M C ^a   Blanco Labra, A ^b   Salazar Olivo, L A ^a,c  

^a UNIVERSIDAD AUTÓNOMA DE QUERÉTARO   (Mexico)

^b DEPARTAMENTO DE FÍSICA   (Mexico)

^c INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA   (Mexico)

Author keywords

Carmine; Cell culture; Colorimetric cell quantitation

Indexed keywords

CARMINIC ACID; FORMALDEHYDE; PHOSPHATE; SODIUM HYDROXIDE;

ABSORPTION; ACCURACY; ANIMAL CELL; ARTICLE; CELL COUNT; CELL DENSITY; CELL GROWTH; CELL TYPE; CHROMOSOME; COLORIMETRY; CONTROLLED STUDY; CORRELATION COEFFICIENT; FIBROBLAST; HUMAN; HUMAN CELL; IN VITRO STUDY; INOCULATION; MOUSE; NONHUMAN; RELIABILITY; SPECTROPHOTOMETRY;

3T3 CELLS; ANIMALS; CARMINE; CELL COUNT; CELL CULTURE TECHNIQUES; CELL LINE, TRANSFORMED; CHICK EMBRYO; COLORIMETRY; COLORING AGENTS; HELA CELLS; HUMANS; INSECTS; MICE; REPRODUCIBILITY OF RESULTS; SPECTROPHOTOMETRY; STAINING AND LABELING;

AVES; INSECTA; MURINAE;

EID: 0036801055     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(02)00044-9     Document Type: Article

References (17)

1. Baranyovits F.L.C. Cochineal carmine. an ancient dye with a modern role Endeavour. 2:1978;85-92.
2. Castro-Muñozledo F., Valencia-García C., Kuri-Harcuch W. Cultivation of rabbit corneal epithelial cells in serum-free medium. Investigative Ophthalmology and Visual Science. 38:1997;2234-2244.
3. Del Castillo P., Llorente A.R., Gomez A., Gonsalvez J., Goyanes V.J., Stockert J.C. New fluorescence reactions in DNA cytochemistry. 2. Microscopic and spectroscopic studies on fluorescent aluminum complexes. Analytical and Quantitative Cytology and Histology. 12:1990;11-20.
4. Freshney R.I. Culture of Animal Cells. 4th Ed:2000;Wiley-Liss, New York.
5. García-Velázquez A. Técnicas y Procedimientos de Citogenética Vegetal. Colegio de Postgraduados. Universidad Autónoma de Chapingo. Montecillo, México, 1990.
6. Gonzalez R.J., Tarloff J.B. Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity. Toxicology in Vitro. 15:2001;257-259.
7. Jabbar S.A., Twentyman P.R., Watson J.V. The MTT assay underestimates the growth inhibitory effects of interferons. British Journal of Cancer. 60:1989;523-528.
8. Margis R., Borojevic R. Quantification of attached cells in tissue culture plates and on microcarriers. Analytical Biochemistry. 181:1989;209-211.
9. Marionnet A.V., Lizard G., Chardonnet Y., Schmitt D. Comparative evaluation of the antiproliferative effect of cyclosporin A and γ-interferon on normal and HPV-transformed keratinocytes by cell counting, MTT assay and tritiated thymidine incorporation. Cell Biology and Toxicology. 13:1997;115-123.
10. Mossman T. Rapid colorimetric assay for cellular growth and survival. application to proliferation and cytotoxicity assays Journal of Immunological Methods. 65:1983;55-63.
11. Oliver M.H., Harrison N.K., Bishop J.E., Cole P.J., Laurent G.J. A rapid and convenient assay for counting cells cultured in microwell plates. application for assessment of growth factors Journal of Cell Science. 92:1989;513-518.
12. Owen H.R., Miller A.R. A comparison of staining techniques for somatic chromosomes of strawberry. HortScience. 28:1993;155-156.
13. Paggliacci M.C., Spinozzi F., Migliorati G., Fumi G., Smacchia M., Grigmnani F., Riccardi C., Nicoletti I. Genistein inhibits tumor cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts. a further pitfall in the use of the MTT assay for evaluating cell growth and survival European Journal of Cancer. 29A:1993;1573-1577.
14. Rieck P., Peters D., Hartmann C., Courtois Y. A new, rapid colorimetric assay for quantitative determination of cellular proliferation, growth inhibition, and viability. Journal of Tissue Culture Methods. 15:1993;37-42.
15. Snow R. Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations. Stain Technology. 38:1963;9-13.
16. Stockert J.C., Llorente A.R., Del Castillo P., Gómez A. Chromatin fluorescence after carmine staining. Stain Technology. 65:1990;299-302.
17. Underwood P.A., Bean P.A. Extracellular matrix interferences with colorimetric estimation of cell number. In Vitro Cellular and Developmental Biology. 34:1998;200-202.