Urine nandrolone metabolites: False positive doping test?

British Journal of Sports Medicine

Volumn 36, Issue 5, 2002, Pages 325-329

  Kohler, R M N ^a   Lambert, M I ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

NANDROLONE;

ATHLETE; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DOPING; HUMAN; METABOLITE; REVIEW; SENSITIVITY AND SPECIFICITY; STEROID METABOLISM; URINE LEVEL;

ANABOLIC AGENTS; AROMATASE; CARBON ISOTOPES; DIETARY SUPPLEMENTS; DOPING IN SPORTS; EXERTION; FALSE POSITIVE REACTIONS; FEMALE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; HYPOGLYCEMIA; MALE; MUSCLE, SKELETAL; NANDROLONE; PHYTOTHERAPY; SPORTS MEDICINE; STRESS;

EID: 0036796849     PISSN: 03063674     EISSN: None     Source Type: Journal    
DOI: 10.1136/bjsm.36.5.325     Document Type: Review

References (77)

1. Le Bizec B, Monteau F, Gaudin I, et al. Evidence for the presence of endogenous 19-norandrosterone in human urine. Journal of Chromatography B 1999;723:157-72.
2. Ryan A. Anabolic androgenic steroids. Berlin, Heidelberg: Springer-Verlag, 1976:515-33.
3. Dollers C. Therapeutic drugs. Edinburgh: Churchill Livingstone, 1991;2:N25-9.
4. Haupt HA, Rovere GD. Anabolic steroids: a review of the literature. Am J Sports Med 1984;12:469-84.
5. Lambert MI, St Clair Gibson A. Anabolic-androgenic steroids: effects on muscle size and strength. South African Journal of Sports Medicine 1995;2:6-9.
6. Titlestad SD, Lambert MI, Schwellnus MP. A survey to determine types and dosages of anabolic androgenic steroids used by competitive bodybuilders in South Africa. South African Journal of Sports Medicine 1994;1:24-8.
7. Hickson RC, Ball KL, Falduto MT. Adverse effects of anabolic steroids. Med Toxicol Adverse Drug Exp 1989;4:254-71.
8. Bjorkhem E, Ek H. Detection and quantitation of 19-norandrosterone in urine by isotope dilution-mass spectrometry. J Steroid Biochem Mol Biol 1982;17:447-51.
9. Belkein L, Shurmeyer T, Hano R, et al. Pharmacokinetics of 19-nortestosterone esters in normal men. J Steroid Biochem Mol Biol 1985;22:623-9.
10. Masse R, Laliberte C, Tremblay L, et al. Gas chromatographic/mass spectrometric analysis of 19-nortestosterone urinary metabolites in man. Biomed Mass Spectrom 1985;12:115-21.
11. Debruyckere G, de Sagher R, De Leenheer A, et al. The impact of nandrolone metabolites occurring in normal male urines, on the cut-off level stipulated for nandrolone doping. In: Gorog S, ed. Proceedings of the fourth symposium on the analysis of steroids, August 1990, Budapest. Budapest: Pécs, Akademie Kiado, 1990:363.
12. Shanzer W, Donike M. Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolites. Analytica Chimica Acta 1993;275:23-48.
13. Ozer D, Temizer A. The determination of nandrolone and its metabolites in the urine by gas chromatography-mass spectrometry. Eur J Drug Metab Pharmocokinet 1997;22:421-5.
14. Ayotte C. Nutritional supplements and doping controls. IAAF New Studies in Athletics 1999;14:37-42.
15. Dehennin L, Bonnaire Y, Plou P. Urinary excretion of 19-norandrosterone of endogenous origin in man: quantitative analysis by gas chromatography-mass spectrometry. Journal of Chromatography B 1999;721:301-7.
16. Uralets V, Gillette P. Over-the-counter δ5 anabolic steroids 5-androsten-3, 17-dione; 5-androsten-3β, 17β-diol; dehydroepiandrosterone; and 19-nor-5-androsten-3, 17-dione: excretion studies in men. J Anal Toxicol 2000;24:188-93.
17. Segura J. Letter of information to the heads of IOC accredited laboratories. Barcelona, August 22 1996.
18. Catlin D, Leder B, Ahrens B, et al. Trace contamination of over-the-counter androstenedione and positive urine test results for a nandrolone metabolite. JAMA 2000;284:2618-21.
19. Debruyckere G, Van Peteghem CH. Influence of the consumption of meat contaminated with anabolic steroids on doping tests. Analytica Chimica Acta 1993;275:49-56.
20. Le Bizec B, Gaudin I, Monteau F, et al. Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites. I. Mass spectrometric detection and quantitaion of 19-norandrosterone and 19-noretiocholanolone in human urine. Rapid Commun Mass Spectrom 2000;14:1058-65.
21. Kicman AT, Brooks RV. A radioimmunoassay for the metabolites of the anabolic steroid nandrolone. J Pharm Biomed Anal 1988;6:473-83.
22. Debruyckere G, de Sagher R, Van Peteghem C. Clostebol-positive urine after consumption of contaminated meat. Clin Chem 1992;38:1869-73.
23. International Olympic Committee. Criteria for reporting low concentrations of anabolic steroids. International Olympic Committee document. 1998;Aug 1:appendix 4.
24. Kintz P, Cirimele V, Ludes B. Norandrosterone and Noretiocholanolone: metabolite markers. Acta Clin Belg Suppl 1999;1:68-73.
25. Reznik Y, Dehennin L, Coffin C, et al. Urinary nandrolone metabolites of endogenous origin in man: a confirmation by output regulation under human chorionic gonadotrophin stimulation. J Clin Endocrinol Metab 2001;86:146-50.
26. Van Eenoo P, Delbeke FT, de Jong FH, et al. Endogenous origin of norandrosterone in female urine: indirect evidence for the production of 19-norsteroids as by-products in the conversion from androgen to estrogen. J Steroid Biochem Mol Biol 2001;78:351-7.
27. Robinson N, Taroni F, Saugy M, et al. Detection of nandrolone metabolites in urine after a football game in professional and amateur players: a Bayesian comparison. Forensic Sci Int 2001;122:130-5.
28. Le Bizec B, Bryand F, Gaudin I, et al. Endogenous nandrolone metabolites in human urine. Two year monitoring of professional soccer male players. J Anal Toxicol 2002;in press.
29. Galan Martin AM, Maynar Marino JI, Garcia de Tiedra MP, et al. Determination of nandrolone metabolites in urine samples from sedentary persons and sportsmen. J Chromatogr B Biomed Sci Appl 2001;761:229-36.
30. Fishman J. Biochemical mechanism of aromatisation. Cancer Res 1982;42:3277-80.
31. Longcope C, Kato T, Horton R. Conversion of blood androgens to estrogens in normal adult men and woman. J Clin Invest 1969;48:2191-201.
32. Ganong WF. Review of medical physiology. Connecticut, USA: Lange Medical Books, 1999;17:283;19:345-60.
33. Sulcova J, Rafter J, Stuka L. 19-nortestosterone in mouse kidney. Endocrinol Exp 1979;13:225-35.
34. Milewich J, Axelrod A. Testosterone metabolism by placental microsomes from baboons: identification of 19-nortestosterone and 19-nor-4-androstenedione. J Steroid Biochem Mol Biol 1979;10:241-3.
35. Dehennin L, Jondet M, Scholler R. Androgen and 19-norsteroid profiles in human preovulatory follicles from stimulated cycles: an isotope dilution-mass spectrometric study. J Steroid Biochem Mol Biol 1987;26:399-405.
36. Reznik Y, Herrou M, Dehennin L, et al. Rising plasma levels of 19-nortestosterone throughout pregnancy: determination by radioimmunoassay and validation by gas chromatography-mass spectrometry. J Clin Endocrinol Metab 1987;64:1086-8.
37. Uralets V, Gillette P. Over-the-counter anabolic steroids 4-androsten-3, 17-dione, 4-androsten 3β, 17β-diol, and 19-nor-4-androsten-3, 17-dione: excretion studies in men. J Anal Toxicol 1999;23:357-66.
38. Makin HJL. Biochemistry of steroid hormones. Blackwell Scientific Publications 1975;11:250-71.
39. Hagensen-Jetne AH, Misund J, Hemmersbach P. Determination of urinary norandrosterone excretion in females during one menstrual cycle by GC/MS. Pittcon Conf. 2000:abstract 585.
40. Mareck-Engelke U, Geyer H, Schanzer W. Recent advances in doping analysis. Cologne: Sport und Buch Strauss, 1998:119.
41. Smals AGH, Pieters GFF, Lozekoot DC, et al. Dissociated response of plasma testosterone and 17 alpha-hydroxyprogesterone to a single or repeated human chorionic gonadotropin administration in normal man. J Clin Endocrinol Metab 1980;50:90.
42. Kicman AT, Brooks RV, Collyer SC, et al. Criteria to indicate testosterone administration. Br J Sports Med 1990;24:253-64.
43. Cowan DA, Kicman AT, Walker CJ, et al. Effect of administration of human chorionic gonadotrophin on criteria used to assess testosterone administration in athletes. J Endocrinol 1991;131:147-54.
44. Padron RS, Wischusen J, Hudson B et al. Prolonged biphasic response of plasma testosterone to a single intramuscular injection of human chorionic gonadotropin. J Clin Endocrinol Metab 1980;50:1000.
45. Pomerantz DK. Human chorionic gonadotropin enhances the ability of gonadotropic hormones to stimulate aromatisation in the testis of the rat. Endocrinology 1981;109:2004.
46. Leder B, Longcope C, Catlin D, et al. Oral androstenedione administration and serum testosterone concentrations in young men. JAMA 2000;283:779-82.
47. Horton R, Tate J. Androstenedione production and the interconversion rates measured in the peripheral blood and studies on the possible site of conversion to testosterone. J Clin Invest 1996;45:301-13.
48. 19-steroids. J Clin Invest 1985;75:1763-9.
49. Gillies H, Derman W, Noakes T, et al. Pseudoephedrine is without ergogenic effects during prolonged exercise. J Appl Physiol 1996;81:2611-17.
50. Sutton JR, Coleman MJ, Casey JH, et al. Androgen response during physical exercise. BMJ 1973;1:520.
51. Lamb DR. Androgens and exercise. Med Sci Sports 1975;1:7.
52. Schmitt WM, Kindermann W, Schnabel A. Testosterone blood level and physical exercise. Int J Sports Med 1982;22(suppl):84.
53. Viruo A. Hormones in muscular activity. Boca Raton, FL:CRC Press, 1985;4:85-9.
54. Vogel R. Increase in free and total testosterone during submaximal exercise in normal males. Med Sci Sport Exerc 1985;17:119-23.
55. MacConnie SE, Barkan A, Lampman RM, et al. Decreased hypothalamic gonadotrophin-releasing hormone secretion in male marathon runners. N Engl J Med 1986;315:411-17.
56. Kraemer RR, Kilgore JL, Kraemer GR, et al. Growth hormone, IGF-1 and testosterone response to restrictive exercise. Med Sci Sports Exerc 1992;4:1346-52.
57. Cumming D, Brunsting L, Strich G, et al. Reproductive hormone increase in response to acute exercise in men. Med Sci Sports Exerc 1986;18:369-73.
58. Wilkerson JE, Horvath SM, Gutin B. Plasma testosterone during treadmill exercise. J Appl Physiol 1980;49:249.
59. Nel S. Springbok rugby player tests positive for steroids. Cape Times 2000:Nov 3:26.
60. Prasad AS, Mantzoros CS, Beck FW, et al. Zinc status and serum testosterone levels in healthy adults. Nutrition 1996;12:344-8.
61. Castro-Magana M, Collipp P, Chen S, et al. Zinc nutritional status, androgens and growth retardation. American Journal of Diseases in Children 1981;135:322-5.
62. Khaled S, Brun JF, Micallel JP, et al. Serum zinc and blood rheology in sportsmen (football players). Clin Hemorheol Microcirc 1997;17:47-58.
63. Hawley J, Dennis S, Lindsay F, et al. Nutritional practices of athletes: are they sub-optimal? J Sports Sci 1995;13:S75-81.
64. Clarkson PM. Minerals: exercise performance and supplementation in athletes. J Sports Sci 1991;9:91-116.
65. Cordova A, Navas FJ. Effect of training on zinc metabolism: changes in serum and sweat zinc concentrations in sportsmen. Ann Nutr Metab 1998;42:274-82.
66. Brilla LR, Conte V. Effect of a novel zinc-magnesium formulation on hormones and strength. Journal of Exercise Physiology 2000;3:26-36.
67. Lewis JG, Ghanadian R, Chisholm GD. Serum dihydrotestosterone and testosterone changes with age in man. Acta Endocrinol 1976;82:444-8.
68. James VHT. UK Sports Council nandrolone review. London: UK Sports Council, January 2000.
69. Morgan WK. On evidence, embellishment and efficacy. J Eval Clin Pract 1997;3:117-22.
70. Saugy M, Robinson N, Cardis C, et al. Nandrolone metabolites in formal competition tests. FIFA Sports Medical Committee.
71. Shackleton CH, Phillips A, Chang T, et al. Confirming testosterone administration by isotope ratio mass spectrometric analysis of urinary androstanediols. Steroids 1997;62:379-87.
72. Shackleton CH, Roitman E, Phillips A, et al. Androstanediol and 5-androstenediol profiling for detecting exogenously administered dihydrotestosterone, epitestosterone and dehydroepiandrosterone: potential use in gas chromatography isotope ratio mass spectrometry. Steroids 1997;62:665-73.
73. Mathurin JC, Herrou V, Bourgogne E, et al. Gas chromatography-combustion-isotope ratio mass spectrometry analysis of 19-norsteroids: application to the detection of a nandrolone metabolite in urine. J Chromatogr B Biomed Sci Appl 2001;759:267-75.
74. Le Bizec B, Bryand F, Gaudin I, et al. Endogenous nandrolone metabolites in human urine. Preliminary results to discriminate between endogenous and exogenous origin. Steroids 2002;67:105-10.
75. Kintz P. Hair testing and doping control in sport. Toxicol Lett 1998;102:109-13.
76. Kintz P, Cirimele V, Dumestre-Toulet V, et al. Doping control for nandrolone using hair analysis. J Pharm Biomed Anal 2001;24:1125-30.
77. Olympic Movement. Olympic Movement anti-doping code 1999: Appendix A: prohibited classes of substances and prohibited methods. Switzerland: IOC Lausanne, 2001.