A pistil-specific thaumatin/PR5-like protein gene of Japanese pear (Pyrus serotina): Sequence and promoter activity of the 5′ region in transgenic tobacco

Plant Molecular Biology

Volumn 50, Issue 3, 2002, Pages 371-377

  Sassa, Hidenori ^a   Ushijima, Koichiro ^a   Hirano, Hisashi ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Anther; Japanese pear; Promoter; Style; Thaumatin like protein; Tobacco

Indexed keywords

CLONING; PROTEINS; TISSUE CULTURE; TOBACCO;

FLORAL ORGANS;

GENES;

5' FLANKING REGION; BASE SEQUENCE; DNA, PLANT; FLOWERS; GLUCURONIDASE; GLYCOPROTEINS; HISTOCYTOCHEMISTRY; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS (GENETICS); PYRUS; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; TOBACCO;

EMBRYOPHYTA; NICOTIANA TABACUM; PYRUS COMMUNIS; PYRUS PYRIFOLIA;

EID: 0036792343     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1019866029629     Document Type: Article

References (31)

1. Bradford, M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
2. Cheung, A.Y., Wu, H.-M., Di Stilio, V., Glaven, R., Chen, C., Wong, E., Ogdahl, J. and Estavillo, A. 2000. Pollen-pistil interactions in Nicotiana tabacum. Ann. Bot. 85: 29-37.
3. Constabel, C.P. and Brisson, N. 1995. Stigma- and vascular-specific expression of the PR 10a gene of potato: a novel pattern of expression of a pathogenesis-related gene. Mol. Plant- Microbe Interact. 8:104-113.
4. Davis, B.J. 1964. Disc electrophoresis-II. Method and application to human serum proteins. Ann. New York Acad. Sci. 121: 404-26.
5. Elliott, R.C., Dickey, L.F., White, M.J. and Thompson, W.F. 1989. cis-acting elements for light regulation of pea ferredoxin I gene expression are located are located within transcribed sequences. Plant Cell 1: 691-698.
6. Ficker, M., Wemmer, T. and Thompson, R.D. 1997. A promoter directing high-level expression in pistils of transgenic plants. Plant Mol. Biol. 35: 425-431.
7. 2-RNase promoter from potato (Solarium tuberosum L.) are required for cell type-specific expression in transgenic potato and tobacco. Mol. Gen. Genet. 257: 132-142.
8. Grenier, J., Potvin, C., Trundel, J. and Asselin, A. 1999. Some thaumatin-like proteins hydrolyse polymeric β-1,3-glucans. Plant J. 19: 473-80.
9. Harikrishna, K., Jampates-Beale, R., Milligan, S.B. and Gasser, C.S. 1996. An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes. Plant Mol. Biol. 30: 899-911.
10. Hon, W.C., Griffith, M., Mlynarz, A., Kwok, Y.A. and Yang, D.C.S. 1995. Antifreeze proteins in winter rye are similar to pathogenesis-related proteins. Plant Physiol. 109: 879-889.
11. Hood, E.E., Gelvin, S.B., Melchers, L.S. and Hoekema, A. 1993. New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2: 208-218.
12. Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G. and Fraley, R.T. 1985. A simple and general method for transferring genes into plants. Science 227: 1229-1231.
13. Jefferson, R.A., Kavanagh, T.A., Bevan, M.W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901-3907.
14. Kim, C.S., Lee, C.H., Shin, J.S., Chung, Y.S. and Hyung, N.I. 1997 A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP. Nucl. Acids Res. 25: 1085-1086.
15. Kosugi, S., Ohashi, Y., Nakajima, K. and Arai, Y. 1990. An improved assay for β-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous β-glucuronidase activity. Plant Sci. 70: 133-140.
16. Kuboyama, T. 1998. A novel thaumatin-like protein gene of tobacco is specifically expressed in the transmitting tissue of stigma and style. Sex. Plant Reprod. 11: 251-256.
17. Kuboyama, T., Yoshida, K.T. and Takeda, G. 1997. An acidic 39-kDa protein secreted from stigmas of tobacco has an amino-terminal motif that is conserved among thaumatin-like proteins. Plant Cell Physiol. 38: 91-95.
18. Maas, C., Laufs, J., Grant, S., Korfhage, C. and Werr, W. 1991 The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to 1000-fold. Plant Mol. Biol. 16: 199-207.
19. McCubbin, A.G. and Kao, T.-H. 2000. Molecular recognition and response in pollen and pistil interactions. Annu. Rev. Cell Dev. Biol. 16: 333-364.
20. 2-ribonuclease) from Nicotiana alata in transgenic Nicotiana tabacum. Plant Cell 4: 1063-1074.
21. Murfett, J., Ebert, P.R., Haring, V. and Clarke, A.E. 1995. An SRNase promoter from Nicotiana alata functions in transgenic N. alata plants but not Nicotiana tabacum. Plant Mol. Biol. 28: 957-963.
22. Ori, N., Sessa, G., Lotan, T., Himmelhoch, S. and Fluhr, R. 1990. A major stylar matrix polypeptide sp41 is a member of the pathogenesis-related proteins superclass. EMBO J. 9: 3429-3436.
23. Sassa, H. and Hirano, H. 1998. Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear (Pyrus serotina Rehd.). Planta 205: 514-521.
24. Sessa, G. and Fluhr, R. 1995. The expression of an abundunt transmitting tract-specific endoglucanase (Sp41) is promoter-dependent and not essential for the reproductive physiology of tobacco. Plant Mol. Biol. 29: 969-982.
25. Sieburth, L.E. and Meyerowitz, E.M. 1997. Molecular dissection of the Agamous control region shows that cis elements for spatial regulation are located intragenetically. Plant Cell 9: 355-365.
26. Singh, N.K., Nelson, D.E., Kuhn, D., Hasegawa, P.M. and Bressan, R.A. 1989. Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiol. 90: 1096-1101.
27. Stintzi, A., Heintz, T., Prasad, V., Wiedemann-Merdinoglu, S., Kauffmann, S., Geoffroy, P., Legrand, M. and Fritig, B. 1993. Plant 'pathogenesis-related' proteins and their role in defense against pathogens. Biochimie 75: 687-706.
28. Ushijima, K., Sassa, H,. Tamura, M., Kusaba, M., Tao, R., Gradziel, T.M., Dandekar, A.M. and Hirano, H. 2001. Characterization of the S-locus region of almond (Prunus dulcis): analysis of a somaclonal mutant and a cosmid contig for an S haplotype. Genetics 158: 379-386.
29. van Eldik, G.J., Wingens, M., Ruiter, R.K., van Herpen, M.M.A., Schrauwen, J.A.M. and Wullems, G.J. 1996. Molecular analysis of a pistil-specific gene expressed in the stigma and stylar cortex of Solanum tuberosum. Plant Mol. Biol. 30: 171-176.
30. Wang, X., Zafian, P., Choudhary, M. and Lawton, M. 1996. The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins. Proc. Natl. Acad. Sci. USA 93: 2598-2602.
31. Wemmer, T., Kaufmann, H., Kirch, H.-H., Schneider, K., Lottspeich, F. and Thompson, R.D. 1994. The most abundant soluble basic protein of the stylar transmitting tract in potato (Solanum tuberosum L.) is an endochitinase. Planta 194: 264-273.