Automated high throughput purification of BigDye™ terminator fluorescent DNA sequencing reactions using Wizard® MagneSil™ paramagnetic particles

JALA - Journal of the Association for Laboratory Automation

Volumn 7, Issue 5, 2002, Pages 75-79

  Otto, Paul ^a,b   Larson, Brad ^a,b   Krueger, Steve ^a,b  

^a PROMEGA CORPORATION   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; DNA; FLUORESCENT DYE; FORMAMIDE; REAGENT; SILICON;

ACCURACY; ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; FLUORESCENCE ANALYSIS; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; PURIFICATION; QUALITY CONTROL; ROBOTICS; SCORING SYSTEM; SEQUENCE ANALYSIS;

EID: 0036776048     PISSN: 15355535     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1535-5535(04)00222-9     Document Type: Article

References (9)

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2. Shiels, G., D. White, and D. Smith. (2001) Automated Plasmid Purification Using MagneSil™ Paramagnetic Particles. Promega Notes (79) 22-4.
3. Bitner, R., S. Koller, H. Shenoi, T. Grunst, and D. Kephart. (2000) Wizard® Magnetic DNA Purification System for Food: Part I. DNA Isolation and Analysis of GMO Foods by PCR. Part II. Semi-Automated DNA Isolation and Analysis of GMO Foods. Promega Notes (76) 14-18.
4. Koller, S., H. Shenoi, J. Burnham, and R. Bitner. (2001) Automated Genomic DNA Purification Using the Wizard® Magnetic 96 DNA Plant System. Promega Notes (79) 25-8.
5. Brisco, P, B. McLaren, D. Creswell, p. Stecha, and R. Hoffman. (2001) Automated Purification of PCR Products Using MagneSil™ Paramagnetic Particles. Promega Notes (79) 18-21.
6. Otto, P. and M. Bjerke. (2001) Automated Purification of BigDye™ Terminator Sequencing Reactions Using Wizard® MagneSil™ Sequencing Reaction Clean-Up System. Promega Notes (79) 2-5.
7. Otto, P. (2002) MagneSil™ Paramagnetic Particles: Magnetics for DNA Purification. JALA (7.3) 34-37.
8. Ewing, B., and P. Green. (1998) Base Calling of Automated Sequencer Traces Using Phred. II Error Probability. Genome Research. 8:186-194.
9. Rosenblum, B.B., L.G. Lee, S.L. Spurgeon, S.H. Kahn, S.M. Menchen, C.R. Heiner and S.M. Chen. (1997) New dye-labeled terminators for improved DNA sequencing patterns. Nucleic Acids Research. 25 (22) 4500-4.