Effect of substrate concentration on short chain fatty acid production in in vitro cultures of human faeces with lactulose, a rapidly fermented carbohydrate

Microbial Ecology in Health and Disease

Volumn 14, Issue 3, 2002, Pages 160-164

  Khan, Khalid M ^a   Edwards, Christine A ^a  

^a Yorkhill Hospitals ^*  (United Kingdom)

Author keywords

Bacteria; Fermentation; Lactulose; Short chain fatty acids

Indexed keywords

CARBOHYDRATE; LACTULOSE; SHORT CHAIN FATTY ACID;

ADULT; ARTICLE; BACTERIAL METABOLISM; CARBOHYDRATE METABOLISM; CONCENTRATION (PARAMETERS); ENZYME SUBSTRATE; FATTY ACID SYNTHESIS; FECES CULTURE; FEMALE; FERMENTATION; HUMAN; IN VITRO STUDY; MALE; MICROBIAL ACTIVITY; MODEL; NORMAL HUMAN; PH; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS);

EID: 0036759066     PISSN: 0891060X     EISSN: None     Source Type: Journal    
DOI: 10.1080/089106002320644348     Document Type: Article

References (25)

1. Cummings JH, Pomare EW, Branch WJ, Naylor CPE, Macfarlane GT. Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut 1987; 28: 1221-7.
2. Hague A, Singh B, Paraskeva C. Butyrate acts as a survival factor for colonic epithelial cells: Further fuel for the in vivo versus in vitro debate. Gastroenterology 1997; 112: 1036-40.
3. Rémésy C, Demigné C, Morand C. Metabolism and utilisation of short chain fatty acids produced by colonic fermentation. In: Schweizer TF, Edwards CA, eds. Dietary Fibre - A Component of Food. London: Springer, 1992: 137-50.
4. Adiotomre J, Eastwood MA, Edwards CA, Brydon WG. Dietary fibre: In vitro methods that anticipate nutrition and metabolic activity in humans. Am J Clin Nutr 1990; 52: 128-34.
5. Barry J-L, Hoebler C, Macfarlane GT, Macfarlane S, Mathers JC, Reed KA, et al. Estimation of the fermentability of dietary fibre in vitro: A European interlaboratory study. Br J Nutr 1995; 74: 303-22.
6. Edwards CA, Gibson G, Champ M, Jensen BB, Mathers JC, Nagengast F, et al. In vitro method for quantification of the fermentation of starch by human faecal bacteria. J Sci Food Agric 1996; 71: 209-17.
7. Mortensen B, Hove H, Rye Clausen M, Holtug K. Fermentation to short chain fatty acids and lactate in human faecal batch cultures: Intra- and inter-individual variations versus variations caused by changes in fermented saccharides. Scand J Gastroenterol 1991; 26: 1285-94.
8. Stevenson A, Buchanan CJ, Abia R, Eastwood MA. A simple in vitro fermentation system for polysaccharides - The effects of fermenter fluid surface area/fluid volume ratio and amount of substrate. J Sci Food Agric 1997; 73: 101-5.
9. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J Nutr 1995; 125: 1401-12.
10. Vince AJ, McNeil NI, Wager ID, Wrong DM. The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system. Br J Nutr 1990; 63: 17-26.
11. Spiller GA, Chernoff MC, Hill RA, Gates JE, Nasser JJ, Shipley EA. Effect of purified cellulose, pectin and a low-residue diet on faecal volatile fatty acids, transit time and faecal weight in humans. Am J Clin Nutr 1980; 33: 754-9.
12. Edwards CA, Duerden BI, Read NW. The effects of pH on colonic bacteria grown in continuous culture. J Med Microbiol 1985; 19: 169-80.
13. Padan A, Zilberstein D, Shuldiner S. pH homeostasis in bacteria. Biochim Biophy Acta 1981; 650: 151-66.
14. Sherman JM, Holm GE. Salt effects in bacterial growth. II. The growth of bacterium coli in relation to H-ion concentration. J Bacteriol 1922; 7: 465-70.
15. Chung KC, Geopfert JM. Growth of Salmonella at low pH. J Food Sci 1970; 35: 326-8.
16. Jay JM. Intrinsic and extrinsic parameters of foods that affect microbial growth. In: Modern Food Microbiology, 4th Edn. New York: Van Nostrand Reinhold, 1996: 38-66.
17. Mortensen PB, Holtug K, Ramussen HS. Short chain fatty acid production from mono- and disaccharides in a fecal incubation system: Implications for colonic fermentation of dietary fibre in humans. J Nutr 1988; 118: 321-5.
18. Daly J, Tomlin J, Read NW. The effect of feeding xanthan gum on colonic function in man: Correlation with in vitro determinants of bacterial breakdown. Br J Nutr 1993; 69: 897-902.
19. Mortensen PB, Nordgaard-Anderson I. The dependence of the in vitro fermentation of dietary fibre to short chain fatty acids on the contents of soluble non-starch polysaccharides. Scand J Gastroenterol 1993; 28: 418-22.
20. McBurney MI, Horvath PJ, Jeraci JL, Van Soest PJ. Effect of in vitro fermentation using human faecal inoculum on the water-holding capacity of dietary fibre. Br J Nutr 1985; 53: 17-24.
21. McBurney MI, Thompson LU. Effect of human faecal inoculum on in vitro fermentation variables. Br J Nutr 1987; 58: 233-43.
22. McBurney MI, Thompson LU. Effect of human faecal donor on in vitro fermentation variables. Scand J Gastroenterol 1989; 24: 359-67.
23. Rasmussen HS, Holtug K, Anderson JR, Krag E, Mortensen PB. The influence of ispaghula husk and lactulose on the in vivo and the in vitro production capacity of short chain fatty acids in humans. Scand J Gastroenterol 1987; 22: 406-10.
24. Salvador V, Cherbut C, Barry J-L, Bertrand D, Bonnet C, Delort-Laval J. Sugar composition of dietary fibre and short chain fatty acid production during in vitro fermentation by human bacteria. Br J Nutr 1993; 70: 189-97.
25. Tomlin J, Read NW. The relation between bacterial degradation of viscous polysaccharides and stool output in human beings. Br J Nutr 1988; 60: 467-75.