In vitro expression of the ctxB toxin gene towards the development of a DNA vaccine against cholera

Molecular Biology Today

Volumn 3, Issue 3, 2002, Pages 71-77

  Syahril, N A ^a   Mariana, N S ^a   Sidhu, H S ^a   Rosli, R ^a  

^a Malaysia   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CHOLERA TOXIN; CHOLERA TOXIN B SUBUNIT; CHOLERA VACCINE; DNA VACCINE; PEPTIDE; REAGENT;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL STRAIN COS7; CELL TRANSPORT; CHOLERA; CONTROLLED STUDY; DIARRHEA; EUKARYOTIC CELL; GENE EXPRESSION; GENE FUNCTION; GENETIC CODE; GENETIC TRANSFECTION; HOST CELL; IMMUNE RESPONSE; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; PROTEIN BINDING; SYMPTOM; VIBRIO CHOLERAE;

ANIMALIA; EUKARYOTA; VIBRIO CHOLERAE;

EID: 0036755379     PISSN: 14685698     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Caulfield, M.J., and Liu, M.A. 1998. Elements of DNA vaccine design. In Stem Cell Biology and Gene Therapy, ed. Quesenberry, P., J., Stein, G., S., Forget, B., G., and Weissman, S., M, pp 309-329. New York: Wiley-Liss, Inc.
2. Fasano, A., Baudry, B., Pumplin, D.W., Wasserman S.S. Tall, B.D., Ketley, J.M., and Kaper, J.B. 1991. Vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions. Proc. Natl. Acad. Sci. U.S.A. 88: 5242-5246.
3. Favre, D., Cryz, Jr.S.J., and Viret, J.F. 1996. Construction and characterization of a potential live oral carrier-based vaccine against Vibrio cholerae O139. Infect. Immun. 64: 3565-3570.
4. Ganguly, N.K., and Kaur, T. 1996. Mechanism of action of cholera toxin and other toxins. Indian J. Med. Res. 104: 28-37.
5. Ghosh, A., Thungapathra, M., and Ghosh, R.K. 1996. Strategies for production of a potential candidate vaccine for cholera. Indian J. Med. Res. 104: 60-75.
6. Heyningen, V.S. 1974. The Subunits of cholera toxin: structure, stoichiometry and function. J. Infect. Dis. 133: 55.
7. Hon, S.K., Syahril, N.A., Mariana, N.S., and Rosli, R. 2000. Detection of the gene encoding the subunit toxin B from Malaysian Vibrio Cholerae isolates. Int. Medical. Journal. 4:45-49.
8. Kowalczyk, D.W., and Ertl, H.C.J. 1999. Immune response to DNA vaccines. Cell Mol. Life Sci. 55: 751-770.
9. Lockman, H., and Kaper, J.B. 1983. Nucleotide sequence analysis of the A2 and B subunits of Vibrio Cholera enterotoxin. J. Biol. Chem. 258: 13722-13726.
10. Mekalanos, J.J., Swartz, D.J., Pearson, G.D.N., Harford, N., Groyne F., and Wilde M. 1983. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature. 306: 551-557.
11. O'brien, A.D., Chen, M.E., Holmes, R.K., Kaper, J.B., and Levine, M.M. 1984. Environmental and human isolates of Vibrio cholerae and Vibrio parahaemolyticus produce a Shigella Dysenteriae 1 (shiga)-like cytotoxin. Lancet 1: 77-78.
12. Olsvik, ØO., Wahlberg, J., Petterson, B., Uhlen, M., Popovic, T., Wachsmuth, I.K., and Fields, P.I. 1993. Use of automated sequencing of polymerase chain reation-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholera O1 strains. J. Clin. Microbial. 31: 22-25.
13. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: A Guide to Methods and Applications. New York: Cold Spring Harbor Laboratory Press.
14. Shroff, K..E., Smith, L.R., Baine, Y., and Higgins, T.J. 1999. Potential for plasmid DNA as vaccines for the New millenium. Pharm. Sci. Tech. 2: 205-212.
15. Spangler, B.D. 1992. Structure and function of cholera toxin and the related Escherichia coli heat-labile enterotoxin. Microbiol. Rev. 56: 622-647.
16. Taylor, D.N., Tacket, C.O., Losonsky, G., Castro, O., Gutierrez, J., Meza, R., Nataro, J.P., Kaper, J.B., Wasserman, S.S., Edelman, R., Levine, M.M, and Cryz., S.J. 1997. Evaluation of a bivalent (CVD 103-HgR/CVD 111) live oral cholera vaccine in adult volunteers from the United States and Peru. Infect. Immune. 65: 3852-3856.
17. The Star. 2001. Five people down with cholera in Klang. http://www.thestar.com.my/news/story.asp?file=/2001/9/4/nation/ klgtaun&newspage.html.
18. Trach, D.D., Clemens, J.D., Ke, N.T., Thuy, H.T., Son, N.D., Canh, D.G., Hang, P.V.D., and Rao M.R. 1997. Field trial of a locally produced, killed, oral cholera vaccine in Vietnam. Lancet. 349: 231-235.
19. Trucksis, M., Galen, J.E., Michalski, J., Fasano, A., and Kaper, J.B. 1993. Accessory cholera enterotoxin (ACE), the third toxin of a Vibrio cholerae virulence cassette. Proc. Natl. Acad. Sci. U.S.A. 90: 5267-71.
20. Varki, A., and Freeze, H. 1994. The major glycosylation pathways of mammalian membranes: A summary. Subcell Biochem. 22: 71-100.
21. Williams, N. A., Hirst, TR., and Nashar, T.O. 1999. Immune modulation by the cholera-like enterotoxins: from adjuvant to therapeutics. Immunology Today. 20: 95-101.
22. World Health Organization (WHO). 2000. Emerging and other communicable diseases, surveillance and control: management of the patients with cholera. http://www.who.int/emc
23. World Health Organization (WHO). 2000. Weekly Epidemiological Record, No. 31.