Antinuclear antibodies (ANA) screening by enzyme immunoassay with nuclear HEp-2 cell extract and recombinant antigens: Analytical and clinical evaluation

Clinical Biochemistry

Volumn 35, Issue 6, 2002, Pages 463-469

  González, Concepción ^a,b   Guevara, Paloma ^a   Alarcón, Inmaculada ^c   Hernando, Monserrat ^a   Navajo, José Alejandro ^a   González Buitrago, José Manuel ^a,b  

^a HOSPITAL UNIVERSITARIO DE SALAMANCA   (Spain)

^b UNIVERSITY OF SALAMANCA   (Spain)

^c HOSPITAL UNIVERSITARIO DE GRAN CANARIA DR NEGRÍN   (Spain)

Author keywords

Antinuclear antibodies; Autoimmune diseases; Enzyme immunoassay; Indirect immunofluorescence

Indexed keywords

ANTINUCLEAR ANTIBODY; CELL EXTRACT; CELL NUCLEUS ANTIGEN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; JO 1 ANTIGEN; RECOMBINANT ANTIGEN; RHEUMATOID FACTOR; SCL 70 ANTIGEN; SMALL NUCLEAR RIBONUCLEOPROTEIN; SS A ANTIGEN; SS B ANTIGEN; UNCLASSIFIED DRUG;

ACCURACY; ANTIBODY SCREENING; ANTIBODY TITER; ANTIGEN SPECIFICITY; AREA UNDER THE CURVE; ARTICLE; AUTOIMMUNE DISEASE; CENTROMERE; CONTROLLED STUDY; ENZYME IMMUNOASSAY; FEMALE; HEP 2 CELL; HUMAN; IMMUNOFLUORESCENCE TEST; IMMUNOGLOBULIN BLOOD LEVEL; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; ROC CURVE; SYSTEMIC LUPUS ERYTHEMATOSUS;

ADULT; ANTIBODIES, ANTINUCLEAR; AUTOIMMUNE DISEASES; CELL EXTRACTS; CELL LINE, TUMOR; CELL NUCLEUS; ENZYMES; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; MALE; MASS SCREENING; SENSITIVITY AND SPECIFICITY;

EID: 0036754680     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(02)00342-9     Document Type: Article

References (15)

1. Von Muhlen C.A., Tan E.M. Autoantibodies in the diagnosis of systemic rheumatic diseases. Sem Arth Rheum. 24:1995;323-358.
2. Kavanaugh A., Tomar R., Reveille R., Solomon D.H., Hamburger H.A. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. Arch Pathol Lab Med. 124:2000;71-81.
3. Humbel R.L. Detection of antinuclear antibodies by immunofluorescence. Van Venroij W.J., Maini R.N., Manual of biological markers of disease. 1993;1-16 Kluwer Academic Publisher, Dordrecht, The Netherlands.
4. Tan E.M., Fettkamp W., Smolen J.S., et al. Range of antinuclear antibodies in healthy individuals. Arthritis Rheum. 40:1997;1601-1611.
5. Tan E.M., Smolen J.S., McDougal J.S., et al. A critical evaluation of enzyme immunoassays for detection of antinuclear antibodies of defined specificities. Arthritis Rheum. 42:1999;455-464.
6. Moncé N.H., Boguski R.T., Cappel N.N. An enzyme immunoassay screening test for the detection of total antinuclear antibodies. J Clin Lab Anal. 5:1991;439-442.
7. Jaskowski T.D., Schroeder C., Martins T.B., et al. Screening for antinuclear antibodies by enzyme immunoassay. Am J Clin Pathol. 105:1996;468-473.
8. Emlen W., O'Neill L. Clinical significance of antinuclear antibodies. Arthritis Rheum. 40:1997;1612-1618.
9. Homburger H.A., Cahen Y.D., Griffiths J., et al. Detection of antinuclear antibodies comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods . Arch Pathol Lab Med. 122:1998;993-999.
10. Bayer P.M., Bauerfeind S., Bienvenu J., et al. Multicenter evaluation study on a new Hep2 ANA screening enzyme immune assay. J Autoimmunity. 13:1999;89-93.
11. Bossuyt X. Evaluation of two automated enzyme immunoassays for detection of antinuclear antibodies. Clin Chem Lab Med. 38:2000;1033-1037.
12. Hayashi N., Kawamoto T., Mukai M., Morinobu A., Koshiba M., Kondo S., Maekawa S., Kumagai S. Detection of antinuclear antibodies by use of an enzymeimmunoassay with nuclear HEp-2 cell extract and recombinant antigens comparison with immunofluorescence assay in 307 patients . Clin Chem. 47:2001;1649-1659.
13. Koch G.G., Landis J.R., Freeman D.H. Jr, Lehnen R.C. A general methodology for the analysis of experiments with repeated measurements of categorical data. Biometrics. 33:1977;133-158.
14. Rizzini M., Reda I., Carabellese N., et al. Comparison of immunofluorescence and ELISA assays for the detection of antinuclear antibodies a multicenter study . Eur J Lab Med. 4:1996;186-192.
15. Nossent H., Rekvig O.P. Antinuclear antibody screening in this new millennium farewell to the microscope? Scand J Rheumatol. 2001;123-126.