Laser capture microdissection in 2-D co-culture models as a tool to study tumor-stroma interactions

BioTechniques

Volumn 33, Issue 3, 2002, Pages 474-475

  Dahse, R ^a   Berndt, A ^a   Haas, K M ^a   Hyckel, P ^a   Bohmer F D ^a   Claussen, U ^a   Kosmehl, H ^a  

^a JENA UNIVERSITY HOSPITAL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL ADHESION; CELL INTERACTION; CELL MIGRATION; CELL TYPE; COCULTURE; CONTROLLED STUDY; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; LASER; MICROENVIRONMENT; STROMA; TUMOR CELL; TUMOR CELL CULTURE; TUMOR GROWTH; CELL CULTURE; CELL SEPARATION; DISSECTION; EVALUATION; INSTRUMENTATION; ISOLATION AND PURIFICATION; LASER SURGERY; METHODOLOGY; MOUTH TUMOR; PATHOLOGY; SQUAMOUS CELL CARCINOMA; STROMA CELL;

RNA;

CARCINOMA, SQUAMOUS CELL; CELL SEPARATION; COCULTURE TECHNIQUES; DISSECTION; FIBROBLASTS; HUMANS; LASER SURGERY; LASERS; MOUTH NEOPLASMS; RNA, NEOPLASM; STROMAL CELLS; TUMOR CELLS, CULTURED;

EID: 0036753464     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02333bm01     Document Type: Article

References (9)

1. Berndt, A., P. Hyckel, A. Konneker, D. Katenkamp, and H. Kosmehl. 1997. Oral squamous cell carcinoma invasion is associated with a laminin-5 matrix re-organization but independent of basement membrane and hemidesmosome formation. Clues from an in vitro invasion model. Invasion Metastasis 17:251-258.
2. Curran, S., J.A. McKay, H.L. McLeod, and G.I. Murray. 2000. Laser capture microscopy. Mol. Pathol. 53:64-68.
3. Dahse, R., A. Berndt, and H. Kosmehl. 2002. Total-RNA-based target design for microarray analysis of defined tumor areas. BioTechniques 32:744-747.
4. Gregoire, M. and B. Lieubeau. 1995. The role of fibroblasts in tumor behavior. Cancer Metastasis Rev. 14:339-350.
5. Kassis, J., D.A. Lauffenburger, T. Turner, and A. Wells. 2001. Tumor invasion as dysregulated cell motility. Semin. Cancer Biol. 11:105-117.
6. Luo, L., R.C. Salunga, H. Guo, et al. 1999. Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat. Med. 5:117-122.
7. Olsen, J., O. Lefebvre, C. Fritsch, J.T. Troelsen, V. Orian-Rousseau, M. Kedinger, and P. Simon-Assmann. 2000. Involvement of activator protein I complexes in the epithelium-specific activation of the laminin γ2-chain gene promoter by hepatocyte growth factor (scatter factor). Biochem. J. 347:407-417.
8. Ruiter, D., T. Bogenrieder, D. Elder, and M. Herlyn. 2002. Melanoma-stroma interactions: Structural and functional aspects. Lancet Oncol. 3:35-43.
9. Sacks, P.G. 1996. Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck. Cancer Metastasis Rev. 15:27-51.