The Mu three-site synapse: A strained assembly platform in which delivery of the L1 transposase binding site triggers catalytic commitment

Molecular Cell

Volumn 10, Issue 3, 2002, Pages 659-669

  Kobryn, Kerri ^a   Watson, Mark A ^a   Allison, Ron G ^a   Chaconas, George ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSPOSASE;

ARTICLE; BINDING SITE; CATALYSIS; CONFORMATIONAL TRANSITION; PROTEIN TARGETING; SYNAPSE;

EID: 0036752746     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00596-8     Document Type: Article

References (43)

1. Aldaz H., Schuster E., Baker T.A. The interwoven architecture of the Mu transposase couples DNA synapsis to catalysis. Cell. 85:1996;257-269.
2. Allison R.G., Chaconas G. Role of the A protein-binding sites in the in vitro transposition of Mu DNA. A complex circuit of interactions involving the Mu ends and the transpositional enhancer. J. Biol. Chem. 267:1992;19963-19970.
3. Baker T.A., Luo L. Identification of residues in the Mu transposase essential for catalysis. Proc. Natl. Acad. Sci. USA. 91:1994;6654-6658.
4. Baker T.A., Mizuuchi K. DNA-promoted assembly of the active tetramer of the Mu transposase. Genes Dev. 6:1992;2221-2232.
5. Chaconas G., Harshey R.M. Transposition of phage Mu DNA. Craig N.L., Craigie R., Gellert M., Lambowitz A. Mobile DNA II. 2002;American Society for Microbiology, Washington, DC. 384-402.pp.
6. Chaconas G., Gloor G., Miller J.L. Amplification and purification of the bacteriophage Mu encoded B transposition protein. J. Biol. Chem. 260:1985;2662-2669.
7. Coros C.J., Chaconas G. Effect of mutations in the Mu-host junction region on transpososome assembly. J. Mol. Biol. 310:2001;299-309.
8. Craigie R., Mizuuchi K. Transposition of Mu DNA. joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu Cell. 51:1987;493-501.
9. Craigie R., Arndt-Jovin D.J., Mizuuchi K. A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition. protein and DNA substrate requirements Proc. Natl. Acad. Sci. USA. 82:1985;7570-7574.
10. Higgins N.P., Collier D.A., Kilpatrick M.W., Krause H.M. Supercoiling and integration host factor change the DNA conformation and alter the flow of convergent transcription in phage Mu. J. Biol. Chem. 264:1989;3035-3042.
11. Jiang H., Harshey R.M. The Mu enhancer is functionally asymmetric both in cis and in trans. Topological selectivity of Mu transposition is enhancer-independent. J. Biol. Chem. 276:2001;4373-4381.
12. Jiang H., Yang J.Y., Harshey R.M. Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. EMBO J. 18:1999;3845-3855.
13. Kobryn K., Lavoie B.D., Chaconas G. Supercoiling-dependent site-specific binding of HU to naked Mu DNA. J. Mol. Biol. 289:1999;777-784.
14. Kobryn K., Naigamwalla D.Z., Chaconas G. Site-specific DNA binding and bending by the Borrelia burgdorferi Hbb protein. Mol. Microbiol. 37:2000;145-155.
15. Krause H.M., Higgins N.P. Positive and negative regulation of the Mu operator by Mu repressor and Escherichia coli integration host factor. J. Biol. Chem. 261:1986;3744-3752.
16. Kuo C.F., Zou A.H., Jayaram M., Getzoff E., Harshey R. DNA-protein complexes during attachment-site synapsis in Mu DNA transposition. EMBO J. 10:1991;1585-1591.
17. Lavoie B.D., Chaconas G. Site-specific HU binding in the Mu transpososome. conversion of a sequence-independent DNA-binding protein into a chemical nuclease Genes Dev. 7:1993;2510-2519.
18. Lavoie B.D., Chaconas G. Transposition of phage Mu DNA. Curr. Top. Microbiol. Immunol. 204:1995;83-99.
19. Lavoie B.D., Chan B.S., Allison R.G., Chaconas G. Structural aspects of a higher order nucleoprotein complex. induction of an altered DNA structure at the Mu-host junction of the Mu type 1 transpososome EMBO J. 10:1991;3051-3059.
20. Lavoie B.D., Shaw G.S., Millner A., Chaconas G. Anatomy of a flexer-DNA complex inside a higher-order transposition intermediate. Cell. 85:1996;761-771.
21. Leung P.C., Teplow D.B., Harshey R.M. Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Nature. 338:1989;656-658.
22. Mariconda S., Namgoong S.Y., Yoon K.H., Jiang H., Harshey R.M. Domain III function of Mu transposase analysed by directed placement of subunits within the transpososome. J. Biosci. 25:2000;347-360.
23. Mizuuchi M., Mizuuchi K. Efficient Mu transposition requires interaction of transposase with a DNA sequence at the Mu operator. implications for regulation Cell. 58:1989;399-408.
24. Mizuuchi M., Mizuuchi K. Conformational isomerization in phage Mu transpososome assembly. effects of the transpositional enhancer and of MuB EMBO J. 20:2001;6927-6935.
25. Mizuuchi M., Baker T.A., Mizuuchi K. DNase protection analysis of the stable synaptic complexes involved in Mu transposition. Proc. Natl. Acad. Sci. USA. 88:1991;9031-9035.
26. Mizuuchi M., Baker T.A., Mizuuchi K. Assembly of the active form of the transposase-Mu DNA complex. a critical control point in Mu transposition Cell. 70:1992;303-311.
27. Mizuuchi M., Baker T.A., Mizuuchi K. Assembly of phage Mu transpososomes - cooperative transitions assisted by protein and DNA scaffolds. Cell. 83:1995;375-385.
28. Naigamwalla D.Z., Chaconas G. A new set of Mu DNA transposition intermediates. alternate pathways of target capture preceding strand transfer EMBO J. 16:1997;5227-5234.
29. Naigamwalla D.Z., Coros C.J., Wu Z., Chaconas G. Mutations in domain III alpha of the Mu transposase. evidence suggesting an active site component which interacts with the Mu-host junction J. Mol. Biol. 282:1998;265-274.
30. Namgoong S.Y., Harshey R.M. The same two monomers within a MuA tetramer provide the DDE domains for the strand cleavage and strand transfer steps of transposition. EMBO J. 17:1998;3775-3785.
31. Pathania S., Jayaram M., Harshey R.M. Path of DNA within the Mu transpososome. transposase interactions bridging two Mu ends and the transposition enhancer trap five DNA supercoils Cell. 109:2002;425-436.
32. Rice P.A., Yang S., Mizuuchi K., Nash H.A. Crystal structure of an IHF-DNA complex. a protein-induced DNA U-turn Cell. 87:1996;1295-1306.
33. Savilahti H., Mizuuchi K. Mu transpositional recombination-donor DNA cleavage and strand transfer in trans by the Mu Transposase. Cell. 85:1996;271-280.
34. Savilahti H., Rice P.A., Mizuuchi K. The phage Mu transpososome core-DNA requirements for assembly and function. EMBO J. 14:1995;4893-4903.
35. Surette M.G., Chaconas G. A protein factor which reduces the negative supercoiling requirement in the Mu DNA strand transfer reaction is Escherichia coli integration host factor. J. Biol. Chem. 264:1989;3028-3034.
36. Surette M.G., Chaconas G. Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the Mu B protein is independent of stable binding of the Mu B protein to DNA. J. Biol. Chem. 266:1991;17306-17313.
37. Surette M.G., Chaconas G. The Mu transpositional enhancer can function in trans. requirement of the enhancer for synapsis but not strand cleavage Cell. 68:1992;1101-1108.
38. Surette M.G., Buch S.J., Chaconas G. Transpososomes. stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA Cell. 49:1987;253-262.
39. Surette M.G., Lavoie B.D., Chaconas G. Action at a distance in Mu DNA transposition. an enhancer-like element is the site of action of supercoiling relief activity by integration host factor (IHF) EMBO J. 8:1989;3483-3489.
40. van Ulsen P., Hillebrand M., Zulianello L., van de Putte P., Goosen N. Integration host factor alleviates the H-NS-mediated repression of the early promoter of bacteriophage Mu. Mol. Microbiol. 21:1996;567-578.
41. Wang Z., Namgoong S.-Y., Zhang X., Harshey R.M. Kinetic and structural probing of the pre-cleavage synaptic complex (type 0) formed during phage Mu transposition. action of metal ions and reagents specific to single stranded DNA J. Biol. Chem. 271:1996;9619-9626.
42. Watson M.A., Chaconas G. Three-site synapsis during Mu DNA transposition. a critical intermediate preceding engagement of the active site Cell. 85:1996;435-445.
43. Williams T.L., Jackson E.L., Carritte A., Baker T.A. Organization and dynamics of the Mu transpososome. recombination by communication between two active sites Genes Dev. 13:1999;2725-2737.