Rapid analysis of protein interactions: On-chip micropurification of recombinant protein expressed in Esherichia coli

Proteomics

Volumn 2, Issue 9, 2002, Pages 1247-1253

  Natsume, Tohru ^a,c   Taoka, Masato ^a   Manki, Hiroshi ^b   Kume, Shouen ^b   Isobe, Toshiaki ^a   Mikoshiba, Katsuhiko ^b  

^a TOKYO METROPOLITAN UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Biomolecular interaction analysis; Inositol 1,4,5 trisphosphate receptor; Matrix assisted laser desorption ionization time of flight mass spectrometry; Surface plasmon resonance biosensor

Indexed keywords

GLUTATHIONE TRANSFERASE; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANTIBODY DETECTION; BINDING KINETICS; CONCENTRATION PROCESS; CONCENTRATION RESPONSE; CONFERENCE PAPER; EPITOPE MAPPING; ESCHERICHIA COLI; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; ONLINE ANALYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; SURFACE PLASMON RESONANCE;

BLOTTING, WESTERN; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; KINETICS; MASS SPECTROMETRY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SURFACE PLASMON RESONANCE; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0036744458     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/1615-9861(200209)2:9<1247::AID-PROT1247>3.0.CO;2-V     Document Type: Conference Paper

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