Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA

Phytotherapy Research

Volumn 16, Issue 6, 2002, Pages 581-585

  Noda, Kiyoshi ^a   Tanaka, Kuniaki ^b   Yamada, Akira ^c   Ogata, Jyunko ^b   Tanaka, Hiroyuki ^a   Shoyama, Yukihiro ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b Chlorella Industry Co Ltd   (Japan)

^c KURUME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Chlorella vulgaris; ELISA; Glycoprotein; Monoclonal antibody; Quantitative analysis

Indexed keywords

ACID; ALKALI; ANTINEOPLASTIC AGENT; BIOLOGICAL RESPONSE MODIFIER; GLYCOPROTEIN; MONOCLONAL ANTIBODY; PROTEINASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CHLORELLA VULGARIS; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FUNGAL STRAIN; IMMUNOREACTIVITY; MOUSE; NONHUMAN; PROTEIN DEGRADATION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY;

CHLORELLA VULGARIS;

EID: 0036741522     PISSN: 0951418X     EISSN: None     Source Type: Journal    
DOI: 10.1002/ptr.1021     Document Type: Article

References (22)

1. Chihara G, Hamuro J, Maeda Y, Arai Y, Fukuoka F. 1970. Fractionation and purification of polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.) Sing (an edible mushroom). Cancer Res 30: 2776-2781.
2. Fott B, Novakova M. A monograph of genus Chlorella. The fresh water species. In Studies in Phytology. Academia Prag. Czechoslovak. 1969, 10-74.
3. Fukuda N, Tanaka H, Shoyama Y. 2000. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rgl and its characterization. Cytotechnology 34: 197-204.
4. Galfre G, Milstein C. Preparation of monoclonal antibodies: Strategies and procedures. In Methods Enzymol, 1981 73: 3-46.
5. Goding JW. 1980. Antibody production by hybridomas. J Immunol Methods 39: 285-308.
6. Ishiyama M, Shoyama Y, Murakami H, Shinohara H. 1996. Production of monoclonal antibodies and development of an ELISA for solamargine. Cytotechnology 18: 153-158.
7. Kikumoto S, Miyajima T, Yoshizumi S, Fujimoto S, Kimura K. 1970. Polysaccharide produced by Schizophyllum commune. Part 1. Formation and some properties of an extracellular polysaccharide. Nippon Nogeikagaku Kaishi 44:337-342.
8. Kobatake H, Suekane T, Murakami Y, Niwa S, Okahira A, Kushida H. 1981. Studies on hot water extract of Mycobacterium tuberculosis. I. Structural analyses of polysaccha rides. Yakugaku-Zasshi 101: 713-722.
9. Konishi F, Mitsuyama M, Okuda M, Tanaka K, Hasegawa T, Nomoto K. 1996. Protective effect of an acidic glycoprotein obtained from culture of Chlorella vulgaris against myelosuppression by 5-fluorouracil. Cancer Immunol Immunother 42: 268-274.
10. Morinaga 0, Tanaka H, Shoyama Y. 2000. Production of monoclonal antibody against a major purgative component, sennoside A, its characterization and ELISA. Analyst 125:1109-1113.
11. Noda K, Ohno N, Tanaka K et al. 1996. A water-soluble glycoprotein from Chlorella vulgaris. Planta Med 62: 423-426.
12. Noda K, Ohno N, Tanaka K et al. 1998. A new type of biological response modifier from Chlorella vulgaris which needs protein moiety to show antitumor activity. Phytother Res 12: 309-319.
13. Okamoto H, Minami M, Shoin S, Koshimura S, Shimizu R. 1966. Experimental anticancer studies. XXXI. On the streptococcal preparation having potent anticancer activity. Jpn J Exp Med 36: 175-186.
14. Putalun W, Tanaka H, Shoyama Y. 1999. Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody. Cytotechnology 31: 151-156.
15. Sakata R, Shoyama Y, Murakami H. 1994. Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, forskolin. Cytotechnology 16: 101-108.
16. Shoyama Y, Fukada T, Murakami H. 1996. Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechnology 19; 55-61.
17. Tanaka H, Fukuda N, Shoyama Y. 1999. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rbl and its characterization. Cytotechnology 29: 115-120.
18. Tanaka H, Goto Y, Shoyama Y. 1996. Monoclonal antibody based enzyme immunoassay for marihuana (cannabinoid) compounds. J Immunoassay 17: 321-342.
19. Tanaka K,Yamada A, Noda K et al. 1998. A novel glycoprotein from Chlorella vulgaris strain CK22 shows antimetastatic immunopotentiation. Cancer Immunol Immunother 45: 313-320.
20. Tsukagoshi S, Hashimoto Y, Fujii G, Kobayashi H, Nomoto K, Orita K. 1984. Krestin (PSK). Cancer Treat Rev 11: 131-155.
21. Weiler EW, Kruger H, Zenk MH. 1976. Radioimmunoassay for the determination of the steroidal alkaloid solasodine and related compounds in living plants and herbarium specimens. Planta Med 39: 112-124.
22. Xuan L, Tanaka H, Xu Y, Shoyama Y. 1999. Preparation of monoclonal antibody against crocin and its characterization. Cytotechnology 29: 65-70.