Probing in vivo metabolism by stable isotope labeling of storage lipids and proteins in developing Brassica napus embryos

Plant Physiology

Volumn 130, Issue 1, 2002, Pages 347-361

  Schwender, Jörg ^a   Ohlrogge, John B ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUXES; GAS CHROMATOGRAPHY; ISOTOPES; LABELING; LIPIDS; MASS SPECTROMETRY; METABOLISM; PROTEINS; SUGAR (SUCROSE);

BIOGENETIC ORIGIN;

PLANTS (BOTANY);

BRASSICA; BRASSICA NAPUS;

EID: 0036740911     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.004275     Document Type: Article

References (62)

1. Asokanthan PS, Johnson RW, Griffith M, Krol M (1997) The photosynthetic potential of canola embryos. Physiol Plant 101: 353-360
2. Bao X, Pollard M, Ohlrogge JB (1998) The biosynthesis of erucic acid in developing embryos of Brassica rapa. Plant Physiol 118: 183-190
3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
4. Christensen B, Nielsen J (1999) Isotope analysis using GC/MS. Metabolic Eng 1: 282-290
5. Das Neves HJC, Vasconcelos AMP (1987) Capillary gas chromatography of amino acids, including asparagine and glutamine: sensitive gas chromatographic-mass spectrometric detection of N,O(S)-tert-butylsimethylsilyl derivatives. J Chromatogr 392: 249-258
6. Dauner M, Sauer U (2000) GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing. Biotechnol Prog 16: 642-649
7. Douce R, Neuburger M (1989) The uniqueness of plant-mitochondria. Annu Rev Plant Physiol Plant Mol Biol 40: 371-414
8. Eastmond P, Kolacna L, Rawsthorne S (1996) Photosynthesis by developing embryos of oilseed rape (Brassica napus L.). J Exp Bot 47: 1763-1769
9. Eastmond PJ, Rawsthorne S (1998) Comparison of the metabolic properties of plastids isolated from developing leaves or embryos of Brassica napus L. J Exp Bot 49: 1105-1111
10. Eastmond PJ, Rawsthorne S (2000) Coordinate changes in carbon partitioning and plastidial metabolism during the development of oilseed rape embryo. Plant Physiol 122: 767-774
11. Eisenreich W, Bacher A (2000) Elucidation of biosynthetic pathways by retrodictive/predictive comparison of isotopomer patterns determined by NMR spectroscopy. In JK Setlow, ed, Genetic Engineering, Principles and Methods, Vol 22. Kluwer Academic/Plenum Publishing, New York, pp 121-153
12. Fatland B, Anderson M, Nikolau BJ, Wurtele ES (2000) Molecular biology of cytosolic acetyl-CoA generation. Biochem Soc Trans 28: 593-595
13. Finkelstein R, Somerville C (1990) 3 classes of abscisic-acid(aba)-insensitive, mutations of Arabidopsis define genes that control overlapping subsets of aba responses. Plant Physiol 94: 1172-1179
14. Finkelstein RR, Crouch M (1986) Rapeseed embryo development in culture on high osmoticum is similar to that in seeds. Plant Physiol 81: 907-912
15. Flanigan I, Collins JG, Arora KK, Macleod JK, Williams JF (1993) Exchange-reactions catalyzed by group-transferring enzymes oppose the quantitation and the unraveling of the identity of the pentose pathway. Eur J Biochem 213: 477-485
16. Focks N, Benning C (1998) Wrinkled1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency-in-the-seed-specific regulation of carbohydrate metabolism. Plant Physiol 118: 91-101
17. Fowler DB, Downey RK (1970) Lipid and morphological changes in developing rapeseed, Brassica napus. Can J Plant Sci 50: 233-247
18. Galili G (1995) Regulation of lysine and threonine synthesis. Plant Cell 7: 899-906
19. Glawischnig E, Gierl A, Tomas A, Bacher A, Eisenreich W (2001) Retrobiosynthetic nuclear magnetic resonance analysis of amino acid biosynthesis and intermediary metabolism. Metabolic flux in developing maize kernels. Plant Physiol 125: 1178-1186
20. Hanson AD, Roje S (2001) One-carbon metabolism in higher plants. Annu Rev Plant Physiol Plant Mol Biol 52: 119-137
21. Hartwell J, Bowsher CG, Emes MJ (1996) Recycling of carbon in the oxidative pentose phosphate pathway in non-photosynthetic plastids. Planta 200: 107-112
22. Hellerstein MK, Neese RA (1999) Mass isotopomer distribution analysis at eight years: theoretical, analytic, and experimental considerations. Am J Physiol 276: E1146-E1170
23. Hill LM, Rawsthorne S (2000) Carbon supply for storage-product synthesis in developing seeds of oilseed rape. Biochem Soc Trans 28: 667-669
24. Hirner B, Fischer WN, Rentsch D, Kwart M, Frommer WB (1998) Developmental control of h+/amino acid permease gene expression during seed development of Arabidopsis. Plant J 14: 535-544
25. Husek P (1991) Amino acid derivatization and analysis in five minutes. FEBS Lett 280: 354-356
26. Ilic-Grubor K, Attree SM, Fowke LC (1998) Comparative morphological study of zygotic and microspore-derived embryos of Brassica napus L. as revealed by scanning electron microscopy. Ann Bot 82: 157-165
27. Johnson RW, Asokanthan PS, Griffith M (1997) Water and sucrose regulate canola embryo development. Physiol Plant 101: 361-366
28. Kang F, Rawsthorne S (1994) Starch and fatty-acid synthesis in plastids from developing embryos of oilseed rape (Brassica napus L.). Plant J 6: 795-805
29. Kang F, Rawsthorne S (1996) Metabolism of glucose-6-phosphate and utilization of multiple metabolites for fatty acid synthesis by plastids from developing oilseed rape embryos. Planta 199: 321-327
30. Ke J, Behal RH, Back SL, Nikolau BJ, Wurtele ES, Oliver DJ (2000) The role of pyruvate dehydrogenase and acetyl-coenzyme a synthase in fatty acid synthesis in developing Arabidopsis seeds. Plant Physiol 123: 497-508
31. 2 refixation characteristics of developing canola seeds and silique wall. Aust J Plant Physiol 25: 377-386
32. King SP, Lunn JE, Furbank RT (1997) Carbohydrate content and enzyme metabolism in developing canola siliques. Plant Physiol 114: 153-160
33. Lee WNP, Bergner EA, Guo ZK (1992) Mass isotopomer pattern and precursor product relationship. Biol Mass Spectrom 21: 114-122
34. Lee WNP, Byerley LO, Bergner EA, Edmond J (1991) Mass isotopomer analysis-theoretical and practical considerations. Biol Mass Spectrom 20: 451-458
35. Liedvogel B, Stumpf PK (1982) Origin of acetate in spinach leaf cell. Plant Physiol 69: 897
36. Lohaus G, Moellers C (2000) Phloem transport of amino acids in two Brassica napus L. genotypes and one B. carinata genotype in relation to their seed protein content. Planta 211: 833-840
37. Lund P (1986) L-glutamine and L-glutamate: UV-method with glutaminase and glutamate dehydrogenase. In HU Bergmeyer, ed, Methods of Enzymatic Analysis, Vol 8. Wiley, New York, pp 357-363
38. Mexal J, Fisher JT, Osteryoung J, Reld CPP (1975) Oxygen availability in polyethylene glycol solutions and its implications in plant-water relations. Plant Physiol 55: 20-24
39. Monnier M (1976) Culture in vitro de l'embryon immature de Capsella bursa pastoris (L.). Rev Cytol Biol Veg 39: 1-120
40. Murphy CM (1993) Mass Spectrometry of Lipids. In F Snyder, ed, Handbook of Lipid Research, Vol 7. Plenum Press, New York, pp 71-130
41. Murphy DJ, Cummis I (1989) Biosynthesis of seed storage products during embryogenesis in rape seed, Brassica napus. J Plant Physiol 135: 63-69
42. Murphy DJ, Keen JN, Osullivan JN, Au DMY, Edwards EW, Jackson PJ, Cummis I, Gibbons T, Shaw CH, Ryan AJ (1991) A class of amphipathic proteins associated with lipid storage bodies in plants and possible similarities with animal serum apolipoproteins. Biochim Biophys Acta 1088: 86-94
43. Norton G (1989) Nature and biosynthesis of storage proteins. In G Röbbelen, RK Downey, A Ashri, eds, Oil Crops of the World. McGraw Hill, New York, pp 165-191
44. Norton G, Harris JF (1975) Compositional changes in developing rape seed. Planta 123: 163-174
45. Ohlrogge J, Browse J (1995) Lipid biosynthesis. Plant Cell 7: 957-970
46. Ohlrogge JB, Pollard MR, Stumpf PK (1978) Studies on biosynthesis of waxes by developing jojoba seed tissue. Lipids 13: 203-210
47. Pollard M, Ohlrogge J (1999) Testing models of fatty acid transfer and lipid synthesis in spinach leaf using in vivo oxygen-18 labeling. Plant Physiol 121: 1217-1226
48. Pomeroy MK, Kramer JKG, Hunt DJ, Keller WA (1991) Fatty acid changes during development of zygotic and microspore-derived embryos of Brassica napus. Physiol Plant 81: 447-454
49. Ratledge C, Bowater MDV, Taylor PN (1997) Correlation of ATP/citrate lyase activity with lipid accumulation in developing seeds of Brassica napus L. Lipids 32: 7-12
50. Roscher A, Kruger NJ, Ratcliffe RG (2000) Strategies for metabolic flux analysis in plants using isotope labelling. J Biotechnol 77: 81-102
51. Rossato L, Laine P, Ourry A (2001) Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns. J Exp Bot 52: 1655-1663
52. Ruuska SA, Girke T, Benning C, Ohlrogge JB (2002) Contrapuntal networks of gene expression during Arabidopsis seed filling. Plant Cell 14: 1191-1206
53. Schmid PC, Johnson SB, Schmid HHO (1988) Determination of ester carbonyl O-18/O-16 ratios in phospholipids by gas chromatography-mass spectrometry. Chem Phys Lipids 46: 165-170
54. Singal HR, Sheoran IS, Singh R (1987) Photosynthetic carbon fixation characteristics of fruiting structures of Brassica campestris L. Plant Physiol 83: 1043-1047
55. 2 fixation support oil synthesis in developing Brassica seeds. J Biosci 20: 49-58
56. Singh BK (1999) Biosynthesis of valine, leucine and isoleucine. In BK Singh, ed, Plant Amino Acids: Biochemistry and Biotechnology. Marcel Dekker, New York, pp 227-247
57. Smith JG (1973) Embryo development in Phaseolus vulgaris: II. Analysis of selected inorganic ions, organic acids, amino acids and sugars in the endosperm liquid. Plant Physiol 51: 454-458
58. Stitt M, Lilley RM, Gerhardt R, Heldt HW (1989) Metabolite levels in specific cells and subcellular compartments of plant leaves. Methods Enzymol 174: 518-552
59. Szyperski T (1998) C-13-NMR, MS and metabolic flux balancing in biotechnology research. Q Rev Biophys 31: 41-106
60. von Wettstein-Knowles PM (1982) Elongase and epicuticular wax biosynthesis. Physiol Veg 20: 797-809
61. Wobus U, Weber H (1999) Sugars as signal molecules in plant seed development. Biol Chem 380: 937-944
62. Wolf AV, Brown MG, Prentiss PG (1972) Concentrative properties of aqueous solutions. In RC Weast, ed, Handbook of Chemistry and Physics, Ed 53. CRC Press, Cleveland, pp D181-D223