Effect of a Sinorhizobium meliloti strain with a modified putA gene on the rhizosphere microbial community of alfalfa

Applied and Environmental Microbiology

Volumn 68, Issue 9, 2002, Pages 4201-4208

  Van Dillewijn, Pieter ^a   Villadas, Pablo J ^a   Toro, Nicolás ^a  

^a CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; DNA; ELECTROPHORESIS; ENZYMES; GELS; GENES; GENETIC ENGINEERING; PLANTS (BOTANY); RNA;

INOCULATION;

BACTERIA;

PRIMER DNA; PROLINE DEHYDROGENASE;

ALFALFA; GENETICALLY MODIFIED ORGANISM; MICROBIAL COMMUNITY; RHIZOSPHERE;

ALFALFA; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; DNA MODIFICATION; GEL ELECTROPHORESIS; GENE AMPLIFICATION; MICROFLORA; NODULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PUTA GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RHIZOSPHERE; SINORHIZOBIUM MELILOTI; SOIL INOCULATION; SPAIN; STRAIN DIFFERENCE;

ANTIBIOSIS; BACTERIAL PROTEINS; DNA, BACTERIAL; MEDICAGO SATIVA; MEMBRANE PROTEINS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RHIZOBIUM; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SINORHIZOBIUM MELILOTI; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS); MEDICAGO SATIVA; NEGIBACTERIA; SINORHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 0036731750     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.9.4201-4208.2002     Document Type: Article

References (52)

1. Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana
2. Physiological and ecological aspects of the interactions between plant roots and rhizosphere soil
3. Field calibration of soil-core microcosms: Ecosystem structural and functional comparisons
4. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
5. High resolution SSCP by optimization of the temperature by transverse TGGE
6. Impact of field release of genetically modified Pseudomonas fluorescens on indigenous microbial populations of wheat
7. Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages
8. Competition for nodulation of legumes
9. Analysis of the dynamics of bacterial communities in the rhizosphere of the chrysanthemum via denaturing gradient gel electrophoresis and substrate utilization patterns
10. Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electropheresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA
11. Direct ribosome isolation from soil to extract bacterial rRNA for community analysis
12. Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis
13. Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis
14. Influence of age and stage of development on the neutral carbohydrate components in root exudates from alfalfa plants grown in a gnotobiotic environment
15. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients
16. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) for studying soil microbial communities
17. Polynucleotide probes that target a hypervariable region of 16S rRNA genes to identify bacterial isolates corresponding to bands of community fingerprints
18. Characterization of a Rhizobium meliloti proline dehydrogenase mutant altered in nodulation efficiency and competitiveness on alfalfa roots
19. The Rhizobium meliloti putA gene: Its role in the establishment of the symbiotic interaction with alfalfa
20. Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov. Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system
21. Establishment of introduced antagonistic bacteria in the rhizosphere of transgenic potatoes and their effect on the bacterial community
22. Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and nontransgenic potato plants
23. Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33
24. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
25. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
26. DGGE/TGGE, a method for identifying genes from natural ecosystems
27. Nearly all single base substitutions in DNA fragments joined to a GC clamp can be detected by denaturing gradient gel electrophoresis
28. Perturbation of maize microflora following seed bacterization with Burkholderia cepacia MCI 7
29. Influence of biocontrol strain Pseudomonas fluorescens CHA0 and its antibiotic-overproducing derivative on the diversity of resident root-colonizing pseudomonads
30. Bacterial origin and community composition in the barley phytosphere as a function of habitat and presowing conditions
31. Frequency and biodiversity of 2,4-diacetylphloroglucinol-producing bacteria isolated from the maize rhizosphere at different stages of plant growth
32. An improved method for purifying DNA from soil for polymerase chain reaction amplification and molecular ecology applications
33. Trifolitoxin production in Rhizobium etli strain CE3 increases competitiveness for rhizosphere colonization and root nodulation of Phaseolus vulgaris in soil
34. Trifolitoxin production increases nodulation competitiveness of Rhizobium etli CE3 under agricultural conditions
35. Effects of bacterial antibiotic production on rhizosphere microbial communities from a culture-independent perspective
36. Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils
37. A new approach to utilize PCR-single-strand-conformation polymorphism for 16S rRNA gene-based microbial community analysis
38. Field lysimeter investigation with luciferase-gene (luc)-tagged Sinorhizobium meliloti strains to evaluate the ecological significance of soil inoculation and a recA -mutation
39. Effect of field inoculation with Sinorhizobium meliloti L33 on the composition of bacterial communities in rhizospheres of a target plant (Medicago saliva) and a non-target plant (Chenopodium album)-linking of 16S rRNA gene-based single-strand conformation polymorphism community profiles to the diversity of cultivated bacteria
40. A biological containment system for Rhizobium meliloti based on the use of recombination-deficient (recA) strains
41. Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils
42. Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: Plant-dependent enrichment and seasonal shifts revealed
43. Phylogenetic analysis and in situ identification of bacteria in activated sludge
44. Failure of inoculant rhizobia to overcome the dominance of indigenous strains for nodule formation
45. Nodulation competitiveness in the Rhizobium-legume symbiosis
46. Expression of tfx and sensitivity to the rhizobial peptide antibiotic trifolitoxin in a taxonomically distinct group of α-proteobacteria including the animal pathogen Brucella abortus
47. Genetics of competition for nodulation of legumes
48. Construction and environmental release of a Sinorhizobium metiloti strain more competitive for alfalfa nodulation
49. Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia
50. Determination of microbial diversity in environmental samples: Pitfalls of PCR-based rRNA analysis
51. Variation of microbial communities in soil, rhizosphere, and rhizoplane in response to crop species, soil type, and crop development