Purification and characterization of a digestive alkaline protease from the larvae of Spilosoma obliqua

Archives of Insect Biochemistry and Physiology

Volumn 51, Issue 1, 2002, Pages 1-12

  Anwar, Adil ^a   Saleemuddin, M ^b  

^a UNIVERSITY OF COLORADO   (United States)

^b ALIGARH MUSLIM UNIVERSITY   (India)

Author keywords

Protease inhibitors; Purification; Spilosoma obliqua, larval gut protease; Synthetic substrate hydrolysis

Indexed keywords

BOVINAE; INSECTA; LEPIDOPTERA; SPILOSOMA;

BENZOYLARGININE 4 NITROANILIDE; CASEIN; PROTEINASE; PROTEINASE INHIBITOR;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; LEPIDOPTERA; METABOLISM; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; TEMPERATURE;

ANIMALS; BENZOYLARGININE NITROANILIDE; CASEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; FRACTIONAL PRECIPITATION; HYDROGEN-ION CONCENTRATION; KINETICS; LEPIDOPTERA; MOLECULAR WEIGHT; PROTEASE INHIBITORS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 0036730899     PISSN: 07394462     EISSN: None     Source Type: Journal    
DOI: 10.1002/arch.10046     Document Type: Article

References (38)

1. Ahmad Z, Saleemuddin M, Siddiqui M. 1976. Alkaline protease in the larvae of the army worm Spodoptera litura. Insect Biochem 6:501-505.
2. Ahmad Z, Saleemuddin M, Siddiqui M. 1980. Purification and characterisation of three alkaline proteases from the larva of army worm Spodoptera litura. Insect Biochem 10:667-673.
3. Andrews P. 1964. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J 91:222-233.
4. Anwar A, Saleemuddin M. 1997. Alkaline pH acting digestive enzymes of Spilosoma obliqua: stability and potential as detergent additives. Biotechnol Appl Biochem 25:43-46.
5. Anwar A, Saleemuddin M. 1998. Alkaline proteases: a review. Bioresource Technol 6:175-183
6. Anwar A, Saleemuddin M. 2000. Alkaline protease from Spilosoma obliqua: potential applications in bio-formulations. Biotechnol Appl Biochem 31:85-89.
7. Anwar A, Saleemuddin M. 2001. Regulation of digestive proteolytic activity in the larvae of Spilosoma obliqua (Lep., Arctiidae). J Appl Entomol 125:577-582.
8. Applebaum SW. 1985. Biochemistry of digestion comparative physiology and pharmacology of insects, vol. IV. In: Kerkut GA, Gilbert LI, editors. Toronto: Pergamon Press. p 279-311.
9. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248-254.
10. Christeller JT, Liang WA, Markwick NP, Burgess EPJ. 1992. Midgut protease activities in 12 phytophagous lepidopteran larvae: dietary and proteases inhibitory interactions. Insect Biochem Mol Biol 22:248-254.
11. DeMartino GN, Croall DE. 1983. Purification and characterization of a calcium-dependent protease from rat liver. Biochemistry 26:6287-6291.
12. Euguchi M, Kuriyama K. 1985. Purification and characterization of membrane bound alkaline proteases from midgut tissue of the silkworm Bombyx mori. J. Biochem (Tokyo) 97:1437-1445.
13. Hamed MMB, Attias J. 1987. Isolation and partial characterization of two alkaline proteases of greater wax moth Galleria mellonella L. Insect Biochem 17:653-658.
14. Hayakawa T, Kondo T, Yamazaki Y, Iinuma Y, Mizuno R. 1980. A simple and specific determination of trypsin in human duodenal juice. Gastroenterol Jpn 15:135-139.
15. Houseman JG, Downe AER, Philogene BJR. 1989. Partial characterization of proteinase activity in the larval midgut of the European corn borer Ostrinia nubilalis. Hubner (lepidoptera: pyralidae). Can J Zool 67:864-868.
16. Ishaaya I, Moore I, Joseph D. 1971. Protease and amylase activity in the larvae of Egyptian cotton worm Spodoptera littoralis. J Insect Physiol 17:945-953
17. Johnston KA, Lee MJ, Gatehouse JA, Anstee JH. 1991. The partial purification and characterization of serine protease activity in the midgut of larval Helicoverpa armigera. Insect Biochem 21:389-397.
18. Johnston KA, Lee MJ, Brough C, Hilder VA, Gatehouse AMR, Gatehouse JA. 1995. Protease activities in the larval mid-gut of Heliothis virescens: evidence for the trypsin and chymotrypsin like enzymes. Insect Biochem Mol Biol. 25: 375-383.
19. Kloke JA, Chan BG. 1982. Effects of cotton condensed tannin on feeding and digestion in the cotton pest Heliothis zea. J. Insect Physiol 28:911-915.
20. Kunitz M. 1947. Crystalline soy bean trypsin inhibitor general properties. J Gen Physiol 30:291-310.
21. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
22. Leach BS, Collawn JF Jr, Fish WW. 1980. Behaviour of glycopeptides with empirical molecular weight estimation methods. 1. In sodium dodecyl sulfate. Biochemistry 25:5734-5741.
23. Lowry OH, Rosenbrough NJ, Farr Al, Randall RJ. 1951. Protein measurement with the folin-phenol reagent. J Biol Chem 193:265-275.
24. Milne R, Kaplan H. 1993. Purification and characterization of a trypsin-like digestive enzyme from Spruce budworm (Christoneura fumifera) responsible for the δ-endotoxin from Bacillus thuringiensis. Insect Biochem Mol Biol 23: 663-673.
25. Ortego F, Ramos SI, Ruiz M, Castanera P. 2000. Characterization of proteases from a stored product mite, Tyrophagus putrescentiae. Arch Insect Biochem Physiol 43:116-124.
26. Peterson AM, Fernando GJP, Wells MA. 1995. Purification characterization and cDNA sequence of an alkaline chymotrypsin from the midgut of Manduca sexta. Insect Biochem Mol Biol 25:765-774.
27. Porath J, Axen R, Ernback S. 1967. Chemical coupling of proteins to agarose. Nature 109:1491-1492.
28. Reek GR, Kramer KJ, Baker JE, Kanost MR, Fabrick JA, Behnke GA. 1997. Proteinase inhibitors and resistance of transgenic plants to insects. In: Carozzin N, Koziel M, editors. Advances in insect control. The role of transgenic plants. London: Taylor & Francis Ltd. p 157-183.
29. Saleemuddin M, Ahmad H, Husain A. 1980. A simple rapid and sensitive procedure for the assay of endoproteases using Coomassie brilliant blue G-250. Analytical Biochem 105:202-206.
30. Samuels RI, Charnley AK, Reynolds SE. 1993. A cuticle degrading proteinase from the moulting fluid of the tobacco hornworm, Manduca sexta. Insect Biochem Mol Biol 23:607-614.
31. Shaw E, Maros-Guia M, Cohen W. 1965. Evidence for an active center histidine in trypsin through the use of a specific reagent 1-choloro-3-tosyl amido-7-amino-2-tosyl-L-lysine. Biochemistry 4:2219-2224.
32. Smith R, Turk V. 1974. Cathepsin D: rapid isolation by affinity chromatography on hemoglobin-agarose resin. Eur J Biochem 48:245-254.
33. Teo LH, Woodring JP. 1985. Digestive enzymes in the house cricket Acheta domesticus with special reference to amylase. Comp Biochem Physiol 82:871-877.
34. Terra WR. 1990. Evolution of digestive systems in insects. Ann Rev Entomol 35:181-200
35. Terra WR, Ferreria C, Jordao BP, Dillon RJ. 1996. Digestive enzymes. In: Lehane MJ, Billingsley RF, editors. Biology of the insect midgut. London: Chapman & Hall. p 153-194.
36. Thomas KK, Nation JL. 1984. Protease amylase and lipase activities in the midgut and hindgut of the cricket Gryllus rubens and mole cricket Scapteriscus acletus. Comp Biochem Physiol 79:297-304.
37. Weber K, Osborn M. 1969. The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. J Biol Chem 244:4406-4412.
38. Zhu CY, Baker JE. 1999. Characterization of midgut trypsin-like enzymes and three trypsinogen cDNAs from the lesser grain borer, Rhyzopertha dominica (Cleoptera: Bostrichidae). Insect Biochem Mol Biol 29:1053-1063.