Modification of pLL/DNA complexes with a multivalent hydrophilic polymer permits folate-mediated targeting in vitro and prolonged plasma circulation in vivo

Journal of Gene Medicine

Volumn 4, Issue 5, 2002, Pages 536-547

  Ward, Christopher M ^a   Pechar, Michal ^c   Oupicky, David ^b   Ulbrich, Karel ^c   Seymour, Leonard W ^b  

^a CHRISTIE HOSPITAL   (United Kingdom)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^c INSTITUTE OF MACROMOLECULAR CHEMISTRY   (Czech Republic)

Author keywords

Folate; Non viral; Plasma; Polymer; Targeted; Vector

Indexed keywords

ALBUMIN; C REACTIVE PROTEIN; ESTER DERIVATIVE; FOLATE RECEPTOR; FOLIC ACID; MACROGOL; PLASMA PROTEIN; PLASMID DNA; POLYLYSINE; POLYMER; SPACER DNA; TRIPEPTIDE DERIVATIVE; DNA; MACROGOL DERIVATIVE; PEPTIDE; PROTEIN SERINE THREONINE KINASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; AQUEOUS SOLUTION; ARTICLE; BLOOD CELL; CHEMICAL MODIFICATION; COMPLEX FORMATION; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG CONJUGATION; FEMALE; GENE EXPRESSION; GENE TARGETING; GENE VECTOR; HELA CELL; HUMAN; HUMAN CELL; HYDROPHILICITY; IN VITRO STUDY; IN VIVO STUDY; MATERIAL COATING; MICROMORPHOLOGY; MOLECULAR STABILITY; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; PROTEIN BINDING; SOLUBILITY; SURFACE PROPERTY; SYSTEMIC CIRCULATION; TRANSGENE; ZETA POTENTIAL; METABOLISM;

DNA; FOLIC ACID; GENE TARGETING; GENETIC VECTORS; HELA CELLS; HUMANS; PEPTIDES; POLYETHYLENE GLYCOLS; POLYMERS; PROTEIN-SERINE-THREONINE KINASES;

EID: 0036730299     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jgm.296     Document Type: Article

References (24)

1. Dash PR, Read ML, Barrett LB, Wolfert MA, Seymour LW. Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery. Gene Ther 1999; 6 643-650.
2. Wolfert MA, Dash PR, Nazarova O, Oupicky D, Seymour LW, Smart S, Strohalm J, Ulbrich K. Polyelectrolyte vectors for gene delivery: influence of cationic polymer on biophysical properties of complexes formed with DNA. Bioconjug Chem 1999; 10: 993-1004.
3. Ward CM, Read ML, Seymour LW. Systemic circulation of poly(L-lysine)/DNA vectors is influenced by polycation molecular weight and type of DNA: differential circulation in mice and rats and the implications for human gene therapy. Blood 2001; 97: 2221-2229.
4. Dash PR, Read ML, Fisher KD, Howard KA, Wolfert M, Oupicky D, Subr V, Strohalm J, Ulbrich K, Seymour LW. Decreased binding to proteins and cells of polymeric gene delivery vectors surface modified with a multivalent hydrophilic polymer and retargeting through attachment of transferring. J Biol Chem 2000; 275: 3793-3802.
5. Fisher KD, Ulbrich K, Subr V, Ward CM, Mautner V, Blakey D, Seymour LW. A versatile system for receptor-mediated gene delivery permits increased entry of DNA into target cells, enhanced delivery to the nucleus and elevated rates of transgene expression. Gene Ther 2000; 7: 1337-1343.
6. Pechar M, Ulbrich K, Subr V, Seymour LW, Schacht EH. Poly(ethylene glycol) multiblock copolymer as a carrier of anticancer drug doxorubicin. Bioconjug Chem 2000; 11: 131-139.
7. Wang S, Low PS. Folate-mediated targeting of antineoplastic drugs, imaging agents, and nucleic acids to cancer cells. J Controlled Release 1998; 53: 39-48.
8. Leamon CP, Low P. Cytotoxicity of momordin-folate conjugates in cultured human cells. J Biol Chem 1992; 267: 24966-24971.
9. Xu B, Wiehle S, Roth JA, Cristiano RJ. The contribution of poly-L-lysine, epidermal growth factor and streptavidin to EGF/PLL/DNA polyplex formation. Gene Ther 1998; 5: 1235-1243.
10. Ward CM, Fisher KD, Seymour LW. Turbidometric analysis of polyelectrolyte complexes formed between poly(L-lysine) and DNA. Colloids Surfaces B 1999; 16: 253-260.
11. Leamon CP, Weigl D, Hendren RW. Folate copolymer-mediated transfection of cultured cells. Bioconjug Chem 1999; 10: 947-957.
12. Lee RJ, Low PS. Delivery of liposomes into cultured KB cells via folate-receptor mediated endocytosis. J Biol Chem 1994; 269 3198-3204.
13. Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E. PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 1999; 6: 595-605.
14. Oupicky D, Howard KA, Konak C, Dash PR, Ulbrich K, Seymour LW. Steric stabilization of poly-L-Lysine/DNA complexes by the covalent attachment of semitelechelic poly[N-(2-hydroxypropyl)-methacrylamide]. Bioconjug Chem 2000; 11: 492-501.
15. Siegal J, Wilson MF, Gewurz H. Interactions of C-reactive protein with the complement system. II. C-reactive protein-mediated consumption of complement by poly-L-lysine polymers and other polycations. J Exp Med 1975; 142: 709-721.
16. Ward CM. Folate-targeted non-viral DNA vectors for cancer gene therapy. Curr Opin Mol Ther 2000; 2: 182-185.
17. Gabizon A, Horowitz AT, Goren D, Tzemach D, Mandelbaum-Shavit F, Qazen MM, Zalipsky S. Targeting folate receptor with folate linked to extremities of poly(ethylene glycol)-grafted liposomes: in vitro studies. Bioconjug Chem 1999; 10: 289-298.
18. Ward CM. PhD thesis, Birmingham University, UK, 2000.
19. Ward CM, Acheson N, Seymour LW. Folic acid targeting of protein conjugates into ascites tumour cells from ovarian cancer patients. J Drug Target 2000; 8: 119-123.
20. Oja CD, Semple SC, Chonn A, Cullis PR. Influence of dose on liposome clearance: critical role of blood proteins. Biochim Biophys Acta Biomembranes 1996; 1281: 31-37.
21. Kaiser K, Marek M, Haselgrubler T, Schindler H, Gruber HJ. Basic studies on heterobifunctional biotin-PEG conjugates with a 3-(4-pyridyldithio)propionyl marker on the second terminus. Bioconjug Chem 1997; 8: 545-551.
22. Home Office. Guidance on the Operation of the Animals (Scientific Procedures) Act 1986. HMSO: London, 1990.
23. Luo J, Smith MD, Lantrip DA, Wang S, Fuchs PL. Efficient syntheses of pyrofolic acid and pteroyl azide, reagents for the production of carboxyl-differentiated derivatives of folic acid. J Am Chem Soc 1997; 119: 10004-10013.
24. Oupicky D, Parker AL, Seymour LW. Laterally stabilized complexes of DNA with linear reducible polycations: strategy for triggered intracellular activation of DNA delivery vectors. J Am Chem Soc 2002; 124: 8-9.