Confocal DNA cytometry: A contour-based segmentation algorithm for automated three-dimensional image segmentation

Cytometry

Volumn 49, Issue 1, 2002, Pages 12-21

  Beliën, Jeroen A M ^a   Van Ginkel, Hielke A H M ^a   Tekola, Paulos ^a   Ploeger, Lennert S ^a   Poulin, Neal M ^a   Baak, Jan P A ^a   Van Diest, Paul J ^a  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

3D reconstruction; Confocal laser scanning microscopy; Cytometry; DNA ploidy; Segmentation; Tissue

Indexed keywords

ALGORITHM; ARTICLE; BREAST CANCER; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOMETRY; DNA CONTENT; HUMAN; HUMAN CELL; IMAGING; LIVER CANCER; ORGAN SIZE; PLOIDY; PRIORITY JOURNAL; TISSUE; BREAST TUMOR; CELL NUCLEUS; CONFOCAL MICROSCOPY; FEMALE; IMAGE CYTOMETRY; IMAGE PROCESSING; INSTRUMENTATION; LIVER TUMOR; METHODOLOGY; PATHOLOGY; THREE DIMENSIONAL IMAGING;

DNA;

ALGORITHMS; BREAST NEOPLASMS; CELL NUCLEUS; DNA, NEOPLASM; FEMALE; HUMANS; IMAGE CYTOMETRY; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; LIVER NEOPLASMS; MICROSCOPY, CONFOCAL; PLOIDIES;

EID: 0036726377     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.10138     Document Type: Article

References (38)

1. Belien JAM, Somi S, de Jong JS, van Diest PJ, Baak JPA. Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer. J Clin Pathol 1999;52:184-192.
2. Belien JAM, Baak JPA, van Diest PJ, van Ginkel AHM. Counting mitoses by image processing in Feulgen stained breast cancer sections: the influence of resolution. Cytometry 1997;29:189.
3. Belien JAM, Baak JPA, van Diest PJ, van Ginkel AHM. Counting mitoses by image processing in Feulgen stained breast cancer sections: the influence of resolution. Cytometry 1997;28:135-140.
4. Sapi Z, Hendricks JB, Haris PG, Wilkinson EJ. Tissue section image-analysis of breast neoplasms - evidence of false aneuploidy. Am J Clin Pathol 1993;99:714-720.
5. Sperb RA, Arnold W, Bahr GF, Loning T, Gebbers JO. Comparative DNA image cytometry in imprint-secretion, cytospin-secretion and tissue section preparations of breast-carcinoma. Anal Cell Pathol 1993;5:265-275.
6. Ji L, Tucker J. DNA measurement of overlapping cell nuclei in thick tissue sections. Anal Cell Pathol 1997;14:41-49.
7. Baak JPA, Thunnissen FBJM, Oudejans CBM, Schipper NW. Potential clinical uses of laser scan microscopy. Appl Optics 1987;26:3413-3416.
8. Rigaut JP, Vassy J, Herlin P, Duigou F, Masson E, Briane D, et al. 3-dimensional DNA image cytometry by confocal scanning laser microscopy in thick tissue blocks. Cytometry 1991;12:511-524.
9. Tekola P, Zhu Q, Baak JPA. Confocal laser microscopy and image processing for 3-dimensional microscopy - technical principles and an application to breast cancer. Hum Pathol 1994;25:12-21.
10. van Ginkel AHM, Baak JPA, Tekola P, Belien JAM, van Diest PJ. Confocal laser scanning microscopy: principles and applications in pathology. In: Kirkham N, Lemoine NR, editors. Progress in pathology. Edinburgh: Churchill Livingston; 1995. p 191-209.
11. Zhu QZ, Tekola P, Baak JPA, Belien JAM. Measurement by confocal laser-scanning microscopy of the volume of epidermal nuclei in thick skin sections. Anal Quant Cytol Histol 1994;16:145-152.
12. Tekola P, Baak JPA, Belien JAM, Brugghe J. Highly sensitive, specific, and stable new fluorescent DNA stains for confocal laser microscopy and image processing of normal paraffin sections. Cytometry 1994;17:191-195.
13. Tekola P, Baak JPA, van Ginkel AHM, Belien JAM, van Diest PJ, Broeckaert MAM, et al. Three-dimensional confocal laser scanning DNA ploidy cytometry in thick histological sections. J Pathol 1996;180:214-222.
14. Czader M, Liljeborg A, Auer G, Ponvit A. Confocal 3-dimensional DNA image cytometry of thick tissue sections. Cytometry 1996;25:246-253.
15. Lockett SJ, Sudar D, Thompson CT, Pinkel D, Gray JW. Efficient, interactive, and three-dimensional segmentation of cell nuclei in thick tissue sections. Cytometry 1998;31:275-286.
16. Roysam B, Ancin H, Bhattacharjya AK, Chisti MA, Seegal R, Turner JN. Algorithms for automated characterization of cell populations in thick specimens from 3-D confocal fluorescence microscopy data. J Microsc 1994;173:115-120.
17. Ancin H, Roysam B, Dufresne TE, Chestnut MM, Ridder GM, Szarowski DH, et al. Advances in automated 3-D image analysis of cell populations imaged by confocal microscopy. Cytometry 1996;25:221-234.
18. Irinopoulou T, Vassy J, Beil M, Nicolopoulou P, Encaoua D, Rigaut JP. Three-dimensional DNA image cytometry by confocal scanning laser microscopy in thick tissue blocks of prostatic lesions. Cytometry 1997;27:99-105.
19. Rodenacker K, Aubele M, Hutzler P, Adiga PSU. Groping for quantitative digital 3-D image analysis: an approach to quantitative fluorescence in situ hybridization in thick tissue sections of prostate carcinoma. Anal Cell Pathol 1997;15:19-29.
20. de Solorzano CO, Rodriguez EG, Jones A, Pinkel D, Gray JW, Sudar D, et al. Segmentation of confocal microscope images of cell nuclei in thick tissue sections. J Microsc 1999;193:212-226.
21. Sarti A, de Solorzano CO, Lockett S, Malladi R. A geometric model for 3-D confocal image analysis. IEEE Trans Biomed Eng 2000;47:1600-1609.
22. Adiga PSU, Chaudhuri BB. An efficient method based on watershed and rule-based merging for segmentation of 3-D histopathological images. Pattern Recog 2001;34:1449-1458.
23. Vincent L, Soille P. Watersheds in digital spaces - an efficient algorithm based on immersion simulations. IEEE Trans Pattern Anal and Machine Intell 1991;13:583-598.
24. Mackin RW, Newton LM, Turner JN, Roysam B. Advances in high-speed, three-dimensional imaging and automated segmentation algorithms for thick and overlappped clusters in cytologic preparations - application to cervical smears. Anal Quant Cytol Histol 1998;20:105-121.
25. Rigaut JP, Vassy J. High-resolution 3-dimensional images from confocal scanning laser microscopy - quantitative study and mathematical correction of the effects from bleaching and fluorescence attenuation in depth. Anal Quant Cytol Histol 1991;13:223-232.
26. Glazer AN, Rye HS. Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection. Nature 1992;359:859-861.
27. Rye HS, Yue S, Wemmer DE, Quesada MA, Haugland RP, Mathies RA, et al. Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes - properties and applications. Nucleic Acids Res 1992;20:2803-2812.
28. Hell S, Reiner G, Cremer C, Stelzer EHK. Aberrations in confocal fluorescence microscopy induced by mismatches in refractive-index. J Microsc 1993;169:391-405.
29. Ridler TW, Calvard S. Picture thresholding using an iterative selection method. IEEE Trans Syst Man Cybernet 1978;SMC-8:630-632.
30. Brakenhoff GJ, Blom P, Barends P. Confocal scanning light microscopy with high aperture lenses. J Microsc 1979;117:219-232.
31. Brakenhoff GJ, van der Voort HTM, VanSpronsen EA, Linnemans WAM, Nanninga N. Three dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy. Nature 1985;317:748-749.
32. Sheppard CJR. Axial resolution of confocal fluorescence microscopy. J Microsc 1989;154:237-241.
33. van Kempen GMP, van Vliet LJ, Verveer PJ, van der Voort HTM. A quantitative comparison of image restoration methods for confocal microscopy. J Microsc 1997;185:354-365.
34. Keller HE. Objective lenses for confocal microscopy. In: Pawley JB, editor. Handbook of biological confocal microscopy. New York: Plenum Press; 1990. p 77-86.
35. Pawley JB. Fundamental limits in confocal microscopy. In: Pawley JB, editor. Handbook of biological confocal microscopy. New York: Plenum Press; 1990. p 15-26.
36. Visser TD, Oud JL, Brakenhoff GJ. Refractive-index and axial distance measurements in 3-D microscopy. Optik 1992;90:17-19.
37. Tsien RY, Waggoner A. Fluorophores for confocal microscopy: photophysics and photochemistry. In: Pawley JB, editor. Handbook of biological confocal microscopy. New York: Plenum Press; 1990. p 169-178.
38. van Kempen GMP, van Vliet LJ. The influence of the regularization parameter and the first estimate on the performance of Tikhonov regularized non-linear image restoration algorithms. J Microsc 2000;198:63-75.