Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains

Revista do Instituto de Medicina Tropical de Sao Paulo

Volumn 44, Issue 5, 2002, Pages 239-244

  Guimarães, Semíramis ^a   Sogayar, Maria Inês T Leme ^a   Franco, Marcello ^b  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Antigens; Axenic strains; Giardia duodenalis; Glycoproteins; Proteins; Trophozoites

Indexed keywords

IMMUNOGLOBULIN G; MEMBRANE PROTEIN; PARASITE ANTIGEN; PROTOZOAL PROTEIN;

ADULT; ANIMAL; ARTICLE; CHEMISTRY; FEMALE; GIARDIA; HOST PARASITE INTERACTION; HUMAN; IMMUNOBLOTTING; IMMUNOLOGY; MALE; PATHOGENICITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABBIT;

ADULT; ANIMALS; ANTIGENS, PROTOZOAN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GIARDIA; HOST-PARASITE RELATIONS; HUMANS; IMMUNOBLOTTING; IMMUNOGLOBULIN G; MALE; MEMBRANE PROTEINS; PROTOZOAN PROTEINS; RABBITS;

EID: 0036725618     PISSN: 00364665     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0036-46652002000500001     Document Type: Article

References (28)

1. BRADFORD, M.M. - A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindings. Analyt. Biochem., 72: 248-254, 1976.
2. CAPON, A.G.; UPCROFT, J.A.; BOREHAM, P.F.L.; COTTIS, L. & BUNDESEN, P.G. - Similarities of Giardia antigens derived from human and animal sources. Int. J. Parasit., 19: 91-98, 1989.
3. CROSSLEY, R. & HOLBERTON, D.V. - Characterization of proteins from cytoskeleton of Giardia lamblia. J. Cell Sci., 59: 81-103, 1983.
4. DEN HOLLANDER, N.; RILLEY, D. & BEFFUS, D. - Immunology of giardiasis. Parasit. today, 4: 124-131, 1988.
5. EDSON, C.M.; FARTHING, M.J.G.; THORLEY-LAWSON, D.A. & KEUSCH, G.T. -An 88,000-Mr Giardia lamblia surface protein which is immunogenic in humans. Infect. Immun., 54: 621-625, 1986.
6. EINFELD, D.A. & STIBBS, H.H. - Identification and characterization of a major surface antigen of Giardia lamblia. Infect. Immun., 46: 377-383, 1984.
7. FORREST, M.; ISSAC-RENTON, J. & BOWIE, W. - Immunoblot patterns of Giardia duodenalis isolates from different hosts and geographical locations. Canad. J. Microbiol., 36: 42-46, 1990.
8. GUIMARÃES, S.; URDANETA, H.; SILVA, E.F. & TAVARES, C.A.P. - Entamoeba histolytica: antigenic characterization of axenic strains from Brazil. Rev. Inst. Med. trop. S. Paulo, 33: 6-11, 1991.
9. HAWKES, R. - Identification of concanavalin-A-binding proteins after sodium dodecyl sulfate-gel electrophoresis and protein blotting. Analyt. Biochem., 123: 143-146, 1982.
10. JACOBSON, R.L. & DOYLE, R.J. - Lectir-parasite interactions. Parasit. today, 12: 55-60, 1996.
11. KEISTER, D.B. - Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans. roy. Soc. trop. Med. Hyg., 77: 487-488, 1983.
12. KUMKUM KHANNA, R.; KHULLER, M.; MEHTA, S. & VINAYAK, V.K. - Plasma membrane associated antigens of trophozoites of axenic Giardia lamblia. Trans. roy. Soc. trop. Med. Hyg., 82: 439-444, 1988.
13. LAEMMLI, U.K. - Cleavage of structural protein during the assembly of the head bacteriophage T4. Nature (Lond.), 277: 680-685, 1970.
14. MEYER, E.A. - Giardia lamblia: isolation and axenic cultivation. Exp. Parasit., 39: 101-105, 1976.
15. MOSS, D.M.; MATHEWS, H.M.; VISVESVARA, G.S.; DICKERSON, J.W. & WALKER, E.M. - Antigenic variation of Giardia lamblia in the feces of Mongolian gerbils. J. clin. Microbiol., 28: 254-257, 1990.
16. NASH, T.E.; GILLIN, F.D. & SMITH, P.D. - Excretory-secretory products of Giardia lamblia. J. Immunol., 131: 2004-2010, 1983.
17. NASH, T.E. & AGGARWAL, A. - Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates. J. Immunol., 136: 2623-2632, 1986.
18. ORTEGA-BARRIA, E.; WARD, H.D.; EVANS, J.E. & PEREIRA, M.E.A. - N-acetyl-D-glucosamine is present in cysts and trophozoites of Giardia lamblia and serves as receptor for wheatgerm agglutinin. Molec. Biochem. Parasit., 43: 151-165, 1990.
19. PEATTIE, D.M.; ALONSO, R.A.; HEIN, A. & CAULFIELD, J.P. - Ultrastructural localization of giardins to the edges of disk microribbons of Giardia lamblia and the nucleotide and deduced protein sequence of alpha giardin. J. Cell Biol., 109: 2323-2335, 1989.
20. ROCHA, M.O.; TOLEDO, V.P.C.P.; MELLO, R.T. et al. - Giardia lamblia: isolation, axenization and characterization of a strain from an asymptomatic patient from Belo Horizonte, MG, Brazil. Mem. Inst. Oswaldo Cruz, 90: 43-44, 1995.
21. SMITH, P.D.; GILLIN, F.D.; KAUSHAL, N.A. & NASH, T.E. - Antigenic analysis of Giardia lamblia from Afghanistan, Puerto Rico, Ecuador, and Oregon. Infect. Immun., 36: 714-719, 1982.
22. THOMPSON, R.C.A.; REYNOLDSON, J.A. & MENDIS, A.H.W. - Giardia and giardiasis. Advanc. Parasit., 32: 71-160, 1993.
23. TOWBIN, H.; STAEHELIN, T. & GORDON, J. - Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. nat. Acad. Sci. (Wash.), 76: 4350-4354, 1979.
24. UDEZULU, I.A.; VISVESVARA, G.S.; MOSS, D.M. & LEITCH, G.J. - Isolation of two Giardia lamblia (WB strain) clones with distinct surface protein and antigenic profiles and differing infectivity and virulence. Infect. Immun., 60: 2274-2280, 1992.
25. WENMAN, W.M.; MEUSER, R.U. & WALLIS, P.M. - Antigenic analysis of Giardia duodenalis strains isolated in Alberta. Canad. J. Microbiol., 32: 926-929, 1986.
26. WENMAN, W.M.; MEUSER, R.U.; NYUGEN, Q. et al. - Characterization of an immunodominant Giardia lamblia protein antigen related to alpha giardin. Parasit. Res., 79: 587-592, 1993.
27. WRAY, W.; BOULIKAS, T.; WRAY, V.P. & HANCOCK, R. - Silver staining of proteins in polyacrylamide gels. Anal. Biochem., 118: 197-203, 1981.
28. WHO - The World Health Report 1996. Geneva, World Health Organization, 1996.