Identification and characterization of cystatin-related epididymal spermatogenic protein in human spermatozoa: Localization in the equatorial segment

Biology of Reproduction

Volumn 67, Issue 3, 2002, Pages 795-803

  Wassler, Michael ^a   Syntin, Patrick ^a   Sutton Walsh, H G ^a   Hsia, Nelson ^a   Hardy, Daniel M ^a   Cornwall, Gail A ^a  

^a TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER SCHOOL OF MEDICINE   (United States)

Author keywords

Acrosome reaction; Fertilization; Gamete biology; Sperm; Spermatogenesis

Indexed keywords

ACETIC ACID; CELL PROTEIN; CYSTATIN RELATED EPIDIDYMAL SPERMATOGENIC PROTEIN; METHANOL; PROTEIN ANTIBODY; UNCLASSIFIED DRUG;

ACROSOME; ARTICLE; CONTROLLED STUDY; EXTRACELLULAR SPACE; FERTILIZATION; GENE EXPRESSION; GERM CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; MALE; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATID; SPERMATOZOON; SPERMATOZOON CAPACITATION; TESTIS; WESTERN BLOTTING;

ACROSOME REACTION; BLOTTING, NORTHERN; CYSTATINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPIDIDYMIS; FERTILIZATION; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION; HUMANS; IN SITU HYBRIDIZATION; MALE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPERM CAPACITATION; SPERMATIDS; SPERMATOZOA; TESTIS;

EID: 0036721358     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod.102.003970     Document Type: Article

References (38)

1. Structure, alternative splicing and chromosomal localization of the cystatin-related epididymal spermatogenic gene
2. The cystatins: Protein inhibitors of cysteine proteinases
3. The disulphide bridges of human cystatin C (γ-trace) and chicken cystatin
4. The CRES gene: A unique testis-regulated gene related to the cystatin family is highly restricted in its expression to the proximal region of the mouse epididymis
5. Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation
6. Cystatin-related epididymal spermatogenic protein colocalizes with luteinizing hormone-β-protein in mouse anterior pituitary gonadotropes
7. Immunolocalization of CRES (cystatin-related epididymal spermatogenic) protein in the acrosomes of mouse spermatozoa
8. Rapid evolution of male reproductive genes in the descent of man
9. The rapid evolution of reproductive proteins
10. B-Myc, a proximal caput epididymal protein, is dependent on androgens and testicular factors for expression
11. Purification and initial characterization of proacrosin from guinea pig testes and epididymal spermatozoa
12. The culture of mouse embryos in vitro
13. 2+-ATPase
14. Initiation of the human sperm acrosome reaction by thapsigargin
15. Mammalian fertilization
16. Mouse sperm antigens that participate in fertilization. I. Inhibition of sperm fusion with the egg plasma membrane using monoclonal antibodies
17. Calcium oscillations in mammalian eggs triggered by a soluble sperm protein
18. Characterization of the antigen recognized by a monoclonal antibody MN9: Unique transport pathway to equatorial segment during spermatogenesis
19. Why did the sperm cross the cumulus? To get to the oocyte. Functions of the sperm surface proteins PH-20 and fertilin in arriving at, and fusing with, the egg
20. An MN9 antigenic molecule, equatorin, is required for successful sperm-oocyte fusion in mice
21. Localization of sperm antigen SP-10 during the six stages of the cycle of the seminiferous epithelium in man
22. A monoclonal antibody to human SP-10 inhibits in vitro the binding of human sperm to hamster oolemma but not to human zona pellucida
23. Rapid inhibition of the sperm protease acrosin by protein C inhibitor
24. Protein C inhibitor may modulate human spermoocyte interactions
25. Monoclonal antibodies which recognize equatorial segment epitopes presented de novo following the A23187-induced acrosome reaction of guinea pig sperm
26. Inhibition of in-vitro fertilization of mouse gametes by proteinase inhibitors
27. Characterization of the mouse sperm plasma membrane zona-binding site sensitive to trypsin inhibitors
28. Evidence for a proteinase inhibitor binding component associated with murine spermatozoa
29. Activation of Xenopus eggs by proteases: Possible involvement of a sperm protease in fertilization
30. p-aminobenzamidine-sensitive acrosomal protease(s) other than acrosin serve the sperm penetration of the egg zona pellucida in mouse
31. Inhibition of sea urchin fertilization by jaspisin, a specific inhibitor of matrix metalloendoproteinase
32. 2+-dependent metalloprotease in mammalian gamete membrane fusion
33. Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease
34. Impaired fertility in mice deficient for the testicular germ-cell protease PC4
35. Proteolytic processing of a protein involved in sperm-egg fusion correlates with acquisition of fertilization competence
36. Processing of the sperm protein Sp17 during the acrosome reaction and characterization as a calmodulin binding protein
37. Mammalian oocyte activation by the synergistic action of discrete sperm head components: Induction of calcium transients and involvement of proteolysis