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Clathrin hub expression dissociates the actin-binding protein Hip1R from coated pits and disrupts their alignment with the actin cytoskeleton
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The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis
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HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2
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The activity of the GTPase-activating protein CdGAP is regulated by the endocytic protein intersectin
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Jenna S., Hussain N.K., Danek E.I., Triki I., Wasiak S., McPherson P.S., Lamarche-Vane N. The activity of the GTPase-activating protein CdGAP is regulated by the endocytic protein intersectin. J Biol Chem. 277:2002;6366-6373. This paper describes the characterization of a Cdc42/Rac-specific GTPase-activating protein (GAP) protein CdGAP, which binds to the SH3 domain of intersectin. Binding of intersectin inhibited the GAP activity of CdGAP, indicating that it could act to increase the levels of activated Cdc42 or Rac.
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Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP
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Hussain N.K., Jenna S., Glogauer M., Quinn C.C., Wasiak S., Guipponi M., Antonarakis S.E., Kay B.K., Stossel T.P., Lamarche-Vane N., McPherson P.S. Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP. Nat Cell Biol. 3:2001;927-932. This paper characterizes a brain-specific splice variant of intersectin that contains additional PH domains and a Dbl homology domain. The authors show that this isoform can catalyze nucleotide exchange on Cdc42.
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