Genotyping of methicillin-resistant Staphylococcus aureus by assaying for the presence of variable elements associated with mecA

Journal of Clinical Microbiology

Volumn 40, Issue 8, 2002, Pages 3093-3097

  Huygens, Flavia ^a   Nimmo, Graeme R ^a   Schooneveldt, Jacqueline ^a   Munckhof, Wendy J ^a   Giffard, Philip M ^a  

^a QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; DNA DETERMINATION; GENOTYPE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

AUSTRALIA; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; CARRIER PROTEINS; DNA TRANSPOSABLE ELEMENTS; GENOTYPE; HEXOSYLTRANSFERASES; HUMANS; METHICILLIN RESISTANCE; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; PENICILLIN-BINDING PROTEINS; PEPTIDYL TRANSFERASES; POLYMERASE CHAIN REACTION; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0036701997     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.8.3093-3097.2002     Document Type: Article

References (22)

1. Bannerman, R. L., G. A. Hancock, F. C. Tenover, and J. M. Miller. 1995. Pulsed-field get electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J. Clin. Microbiol. 33:551-555.
2. Bergeron, M. G., and M. Ouellette. 1998. Preventing antibiotic resistance through rapid genotypic identification of bacteria and of their antibiotic resistance genes in the clinical microbiology laboratory. J. Clin. Microbiol. 36:2169-2172.
3. Corso, A., I. Santos Sanches, M. Aires de Sousa, A. Rossi, and H. de Lencastre. 1998. Spread of a methicillin-resistant and multiresistant epidemic clone of Staphylococcus aureus in Argentina. Microb. Drug Resist. 4:277-288.
4. Cox, R. A., C. Conquest, C. Mallaghan, and R. R. Marples. 1995. A major outbreak of methicillin-resistant Staphylococcus aureus caused by a new phage type (EMRSA-16). J. Hosp. Infect. 29:87-106.
5. Day, N. P. J., C. E. Moore, M. C. Enright, A. R. Berendt, J. M. Smith, M. F. Murphy, S. J. Peacock, B. J. Spratt, and E. J. Feil. 2001. A link between virulence and ecological abundance in natural populations of Staphylococcus aureus. Science 292:114-116.
6. de Lencastre, H., E. P. Severina, R. B. Roberts, B. N. Kreiswirth, A. Tomasz, et al. 1996. Testing the efficacy of a molecular surveillance network: Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF) genotypes in six hospitals in the metropolitan New York City area. Microb. Drug Resist. 2:343-351.
7. de Sousa, M. A., 1. S. Sanches, M. L. Ferro, M. J. Vaz, Z. Saraiva, T. Tendeiro, J. Serra, and H. de Lencastre. 1998. Intercontinental spread of a multidrug-resistant methicillin-resistant Staphylococcus aureus clone. J. Clin. Microbiol. 36:2590-2596.
8. Dominguez, M. A., H. de Lencastre, J. Linares, and A. Tomasz. 1994. Spread and maintenance of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone during an outbreak of MRSA disease in a Spanish hospital. J. Clin. Microbiol. 32:2081-2087.
9. Enright, M. C., N. P. J. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015.
10. Hiramatsu, K., L. Cui, M. Kuroda, and T. Ito. 2001. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends Microbiol. 9:486-493.
11. Jorgensen, M., R. Givney, M. Pegler, A. Vickery, and G. Funnel. 1996. Typing multidrug-resistant Staphylococcus aureus: Conflicting epidemiological data produced by genotypic and phenotypic methods clarified by phylogenetic analysis. J. Clin. Microbiol. 34:398-403.
12. Kreiswirth, B., J. Kornblum, R. D. Arbeit, W. Eisner, J. N. Maslow, A. McGeer, D. E. Low, and R. P. Novick. 1993. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science 259:227-230.
13. Leski, R., D. Oliveira, K. Trzcinski, I. S. Sanches, M. A. de Sousa, W. Hryniewicz, and H. de Lencastre. 1998. Clonal distribution of methicillin-resistant Staphylococcus aureus in Poland. J. Clin. Microbiol. 36:3532-3539.
14. Matsuhashi, M., M. D. Song, F. Ishino, M. Wachi, M. Doi, M. Inoue, K. Ubukata, N. Yamashita, and M. Konno. 1986. Molecular cloning of the gene of a penicillin binding protein supposed to cause high resistance to β-lactam antibiotics in Staphylococcus aureus. J. Bacteriol. 167:975-980.
15. Nimmo, G. R., J. Schooneveldt, G. O'Kane, B. McCall, and A. Vickery. 2000. Community acquisition of gentamicin-sensitive methicillin-resistant Staphylococcus aureus in Southeast Queensland, Australia. J. Clin. Microbiol. 38:3926-3931.
16. Oliveira, D., 1. S. Sanches, M. Tamayo, G. Ribeiro, R. Maro, D. Costa, and H. de Lencastre. 1998. Virtually all MRSA infections in the largest Portuguese hospital are caused by two internationally spread multiresistant strains: The "Iberian" and the "Brazilian" clones of MRSA. Clin. Microbiol. Infect. 4:373-384.
17. Oliveira, D. C., S. W. Wu, and H. de Lencastre. 2000. Genetic organization of the downstream region of the mecA element in methicillin-resistant Staphylococcus aureus isolates carrying different polymorphisms of this region. Antimicrob. Agents Chemother. 44:1906-1910.
18. O'Neill, G. L., S. Murchan, A. Gil-Setas, and H. M. Aucken. 2001. Identification and characterization of phage variants of a strain of epidemic methicillin-resistant Staphylococcus aureus (EMRSA-15). J. Clin. Microbiol. 39:1540-1548.
19. Richardson, J. F., and S. Reith. 1993. Characterization of a strain of methicillin-resistant Staphylococcus aureus (EMRSA-15) by conventional and molecular methods. J. Hosp. Infect. 25:45-52.
20. Roman, R. S., J. Smith, M. Walker, S. Byrne, K. Ramotar, B. Dyck, A. Kabai, and L. E. Nicolle. 1996. Rapid geographic spread of a methicillin-resistant Staphylococcus aureus strain. Clin. Infect. Dis. 25:698-705.
21. Ryffel, C., R. Bucher, F. H. Kayser, and B. Berger-Bachi. 1991. The Staphylococcus aureus mec determinant comprises an unusual cluster of direct repeats and codes for a gene product similar to the Escherichia coli snglycerophosphoryl diester phosphodiesterase. J. Bacteriol. 173:7416-7422.
22. Tenover, F., R. Arbeit, R. Goering, P. Mickelsen, B. Murray, D. Persing, and B. Swaminathan. 1995. Interpreting chromosomal CAN restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J. Clin. Microbiol. 33:2333-2339.