Clean SEA-HSQC: A method to map solvent exposed amides in large non-deuterated proteins with gradient-enhanced HSQC

Journal of Biomolecular NMR

Volumn 23, Issue 4, 2002, Pages 317-322

  Lin, Donghai ^a,b   Sze, Kong Hung ^a   Cui, Yangfang ^a   Zhu, Guang ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

^b SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Amide hydrogen exchange; CLEAN PM; HSQC; Proteins; Resonance overlap; Solvent exposed amides

Indexed keywords

AMIDE; AMINE; DEUTERIUM; HYDROXYL GROUP; LIGAND; NITROGEN 15; PROTON; SOLVENT; NITROGEN; PROTEIN;

ANALYTIC METHOD; ARTICLE; ARTIFACT; CONTAMINATION; CONTROLLED STUDY; EXPOSURE; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN ANALYSIS; TRANSPORT KINETICS; CHEMISTRY; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PEPTIDE MAPPING;

AMIDES; DEUTERIUM; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDE MAPPING; PROTEINS; PROTONS; SOLVENTS;

EID: 0036700882     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020225206644     Document Type: Article

References (18)

1. Bai, Y., Milne, J.S., Mayne, L. and Englander, S.W. (1993) Protein Struct. Func. Gen., 17, 75-86.
2. Breeze, A.L. (2000) Prog. NMR Spectrosc., 36, 323-372.
3. Delaglio, F., Grzesiek, S., Wister, G.W., Zhu, G., Pfeifer, J. and Bax, A. (1995) J. Biomol. NMR, 6, 277-293.
4. Dempsey, C.E. (2001) Prog. NMR Spectrosc., 39, 135-170.
5. Gemmecker, G., Jahnke, W. and Kessler, H. (1993) J. Am. Chem. Soc., 115, 11620-11621.
6. Gemmecker, G., Olejniczak, E.T. and Fesik, S.W. (1992) J. Magn. Reson., 96, 199-204.
7. Grzesiek, S. and Bax, A. (1993) J. Biomol. NMR, 3, 627-638.
8. Hwang, T.-L., Mori, S., S. Shaka, A.J. and van Zijl, P. C.M. (1997) J. Am. Chem. Soc., 119, 6203-6204.
9. Hwang, T.-L., van Zijl, P. C.M. and Mori, S. (1998) J. Biomol. NMR, 11, 221-226.
10. Ikura, M. and Bax, A. (1992) J. Am. Chem. Soc., 114, 2433-2400.
11. Mori, S., Abeygunawardana, C., Berg, J.M. and van Zijl, P.C.M. (1997) J. Am. Chem. Soc., 119, 6844-6852.
12. Mori, S., Berg, J.M. and van Zij], P.C.M. (1996) J. Biomol. NMR, 7, 77-82.
13. Otting, G.. and Wüthrich, K. (1990) Quart. Rev. Biophys., 23, 39-96.
14. Pellecchia, M., Meininger, D., Shen, A.L., Jack, R., Kasper, C.B. and Sem, D.S. (2001) J. Am. Chem. Soc., 123, 4633-4634.
15. Shaka, A.J., Barker, P.B. and Freeman, R. (1985) J. Magn. Reson., 64, 547-552.
16. Sklenar, V. (1995), J. Magn. Reson., A114, 132-135.
17. Wang, Y., Qian, R.Q. and Gu, Z-W. et al. (1986) Pure Appl. Chem., 58, 789-798.
18. Zhu, G., Kong, X. and Sze, K. (1999). J. Biomol. NMR, 13, 77-81.