Sentinel lymph node in melanoma: Use of molecular biology (RT-PCR) in the detection of occult metastases

Pathologica

Volumn 94, Issue 4, 2002, Pages 190-195

  Riccioni, L ^a   Farabegoli, P ^b   Nanni, O ^c   Morigi, F ^a   Polverelli, M ^b   Landi, G ^b   Giangaspero, F ^a  

^a Unità Operativa di Anatomia Patologica   (Italy)

^b BUFALINI HOSPITAL   (Italy)

^c ISTITUTO ONCOLOGICO ROMAGNOLO   (Italy)

Author keywords

Melanoma; Metastasis; RT PCR; Sentinel lymph node; Tyrosinase

Indexed keywords

MESSENGER RNA; MONOPHENOL MONOOXYGENASE; DIAGNOSTIC AGENT; MACROSALB TC 99M; MELANOMA SPECIFIC ANTIGENS; MELANOMA-SPECIFIC ANTIGENS; PROTEIN S 100; RADIOPHARMACEUTICAL AGENT; RNA; TECHNETIUM TC 99M NANOCOLLOID; TUMOR ANTIGEN; TUMOR MARKER; TUMOR PROTEIN;

ADULT; ARTICLE; CANCER STAGING; CONTROLLED STUDY; CORRELATION ANALYSIS; FEMALE; HISTOLOGY; HUMAN; IMMUNOHISTOCHEMISTRY; ITALY; LYMPH NODE METASTASIS; MAJOR CLINICAL STUDY; MALE; MELANOMA; MELANOMA CELL; NEVUS CELL; OCCULT CANCER; PATIENT MONITORING; PROGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY ANALYSIS; SENTINEL LYMPH NODE; TUMOR VOLUME; CHEMISTRY; COMPARATIVE STUDY; EVALUATION; GENETICS; METASTASIS; MIDDLE AGED; PATHOLOGY; SCINTISCANNING; SENSITIVITY AND SPECIFICITY; SENTINEL LYMPH NODE BIOPSY;

ADULT; ANTIGENS, NEOPLASM; FEMALE; HUMANS; LYMPHATIC METASTASIS; MALE; MELANOMA; MIDDLE AGED; MONOPHENOL MONOOXYGENASE; NEOPLASM PROTEINS; NEOPLASM STAGING; RADIOPHARMACEUTICALS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; S100 PROTEINS; SENSITIVITY AND SPECIFICITY; SENTINEL LYMPH NODE BIOPSY; TECHNETIUM TC 99M AGGREGATED ALBUMIN; TUMOR MARKERS, BIOLOGICAL;

EID: 0036692978     PISSN: 00312983     EISSN: None     Source Type: Journal    
DOI: 10.1007/s102420200030     Document Type: Article

References (24)

1. Detection of submicroscopic lymph node metastases in patients with malignant melanoma
2. Molecular test for the detection of tumor cells in blood and sentinel nodes of melanoma patients
3. Molecular staging of malignant melanoma. Correlation with clinical outcome
4. Final version of the American Joint Commitee on Cancer Staging System for Cutaneous Melanoma
5. Recommendations for handling radioactive specimens obtained by sentinel lymphadenectomy
6. Detection of melanoma cells in peripheral blood by means of reverse trancriptase and polymerase chain reaction
7. Pathologic examination of the sentinel lymph node in malignant melanoma
8. Surgical pathology remains pivotal in the evaluation of "sentinel" lymph nodes
9. Occult tumor cell in the lymph nodes of patients with pathological stage I malignant melanoma. An immunohistological study
10. HMB-45 immunohistochemical staining of sentinel lymph nodes: A specific method for enhancing detection of micrometastases in patient with melanoma
11. Recent advances in chemistry of melanogenesis in mammals
12. Molecular mechanism of tyrosinase regulation by L-DOPA in hamster melanoma cells
13. Sentinel node biopsy and selective dissection for melanoma nodal metastases
14. Reliability of sentinel lymph node biopsy for staging melanoma
15. Detection of melanoma micrometastasis in sentinel nodes by reverse transcription-polymerase chain reaction correlates with tumor thickness and is predictive of micrometastatic disease in lymph node basin
16. Detection of mRNA by reverse transcription-polymerase chain reaction in melanoma sentinel nodes
17. Clinical relevance of molecular staging for melanoma. Comparison of RT-PCR and immunohistochemistry staining in sentinel lymph node of patients with melanoma
18. Prognostic significance of occult metastases detected by sentinel lymphadenectomy and reverse transcriptase-polymerase chain reaction in early-stage melanoma patients
19. Prediction of sentinel lymph node micrometastasis by histological features in primary cutaneous malignant melanoma
20. Predicting sentinel and residual basin disease after sentinel lymph node biopsy for melanoma
21. Results of complete lymph node dissection in 83 melanoma patients with positive sentinel nodes
22. Detection of melanoma micrometastases in the sentinel lymph node and in nonsentinel nodes by tyrosinase polymerase chain reaction
23. The detection of circulating melanoma cells correlates with tumor thickness and ulceration but is not predictive for metastasis for patients with primary melanoma
24. Sentinel node biopsies in melanoma patients: A protocol for accurate, efficient, and cost effective analysis by preselection for immunohistochemistry on the basis of Tyr-PCR