6-phosphofructokinase from Pichia pastoris: Purification, kinetic and molecular characterization of the enzyme

Yeast

Volumn 19, Issue 11, 2002, Pages 933-947

  Kirchberger, Jürgen ^a   Bär, Jörg ^a   Schellenberger, Wolfgang ^a   Dihazi, Hassan ^a   Kopperschläger, Gerhard ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

6 phosphofructokinase; Methylotrophic yeast; Molecular and kinetic properties; Pfk; Pichia pastoris; Purification

Indexed keywords

6 PHOSPHOFRUCTOKINASE; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE; ARGININE; ASPARAGINE; ASPARTIC ACID; CIBACRON BLUE F3GA; FRUCTOSE 2,6 BISPHOSPHATE; FRUCTOSE 6 PHOSPHATE; GLUTAMIC ACID; GLUTAMINE; GLYCINE; HISTIDINE; ISOLEUCINE; LEUCINE; LYSINE; METHIONINE; OLIGOMER; PHENYLALANINE; SEPHAROSE; SERINE; THREONINE; VALINE;

AFFINITY CHROMATOGRAPHY; ALLOSTERISM; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBUNIT; KLUYVEROMYCES; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE BINDING SITE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SPECIES DIFFERENTIATION;

AMINO ACID SEQUENCE; CULTURE MEDIA; ENZYME ACTIVATION; GLUCOSE; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHOSPHOFRUCTOKINASE-1; PICHIA;

KLUYVEROMYCES; KLUYVEROMYCES LACTIS; PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036678896     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.885     Document Type: Article

References (40)

1. Studies on the function of yeast phosphofructokinase subunits by in vitro mutagenesis
2. Enhanced detection of phosphopeptides in matrix-assisted laser desorption/ionization mass spectrometry using ammonium salts
3. Purification and characterization of phosphofructokinase from the yeast Kluyveromyces lactis
4. Denaturation of phosphofructokinase-1 from Saccharomyces cerevisiae by guanidium chloride and reconstitution of the unfolded subunits to their catalytically active form
5. Phosphofructokinase from E. coli: Evidence for a tetrameric structure of the enzyme
6. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding
7. Pichia pastoris as a host system for transformation
8. Two-layer sample preparation: A method for MALDI-MS analysis of complex peptide and protein mixtures
9. Protein purification using immobilized triazine dyes
10. One-step purification of recombinant yeast 6-phosphofructo-2-kinase after the identification of contaminants by MALDI-TOF MS
11. Protein identification based on matrix assisted laser desorption/ionization-post source decay-mass spectrometry
12. Phosphofructokinase: Structure and function
13. Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast
14. The phosphofructokinase genes of yeast evolved from two duplication events
15. Yeasts
16. Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis
17. Metabolic regulation and mathematical models
18. Immunological evidence for non-identical subunits in yeast phosphofructokinase
19. The significance of phosphofructokinase to the regulation of carbohydrate metabolism
20. The primary structure of phosphofructokinase from Bacillus stearothermophilus
21. Affinity partitioning
22. Allosteric regulatory properties of muscle phosphofructokinase
23. Assembly of phosphofructokinase-1 from Saccharomyces cerevisiae in extracts of single-deletion mutants
24. Physico-chemical parameters and subunit composition of yeast phosphofructokinase
25. Cibacron Blue F3G-A and related dyes as ligands in affinity chromatography
26. Limited proteolysis of yeast phosphofructokinase. Sequence locations of cleavage sites created by the actions of different proteinases
27. Affinity extraction with dye ligands
28. Effects of specific binding reactions on the partitioning behaviour of biomaterials
29. Phosphofructokinases from Escherichia coli
30. The importance of ATP as a regulator of the glycolytic flux in Saccharomyces cerevisiae
31. Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans
32. Self-associating Systems in the Analytical Ultracentrifuge
33. On the nature of allosteric transitions: A plausible model
34. Similarity of activation of yeast phosphofructokinase by AMP and fructose 2,6-bisphosphate
35. Evolution of phosphofructokinase - Gene duplication and creation of new effector sites
36. Purification, molecular and kinetic characterization of phosphofructokinase-1 from the yeast Schizosaccharomyces pombe: Evidence for an unusual subunit composition
37. Single point mutations in either gene encoding the subunits of the hetero-octameric yeast phosphofructokinase abolish inhibition by ATP
38. Multimodulation of enzyme activity
39. Glucose-induced microautophagy in Pichia pastoris requires the α-subunit of phosphofructokinase