Expression and refolding of an anti-tumor antibody AA98 fragment VH/κ

Progress in Biochemistry and Biophysics

Volumn 29, Issue 4, 2002, Pages 645-650

  Zhang, Zhi Qiang ^a   Lin, Yun ^a   Yan, Xi Yun ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Antibody fragment; Chromatography; Gel refolding

Indexed keywords

ANTIBODY AA98; ARGININE; BUFFER; CANCER ANTIBODY; GLUTATHIONE; GLUTATHIONE DISULFIDE; UNCLASSIFIED DRUG;

ARTICLE; DENATURATION; DILUTION; FLOW RATE; GEL FILTRATION CHROMATOGRAPHY; IN VITRO STUDY; PH;

EID: 0036672843     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. The purification of eukaryotic polypeptides synthesized in Escherichia coli
2. Urea gradient gel filtration chromatography enhanced the protein refolding
3. Refolding proteins by gel filtration chromatography
4. The measure of relative antibody affinity by ELISA using thiocyanate elution
5. Advances in refolding of proteins produced in E. coli
6. H/κ) expressed in E.coli