An immunoenzymatic solid-phase assay for quantitative determination of HIV-1 protease activity

Analytical Biochemistry

Volumn 307, Issue 1, 2002, Pages 18-24

  Gutiérrez, Omar A ^a   Salas, Emir ^a   Hernández, Yanko ^b   Lissi, Eduardo A ^c   Castrillo, Gabriel ^a   Reyes, Osvaldo ^d   Garay, Hilda ^d   Aguilar, Arístides ^d   García, Beatriz ^a   Otero, Anselmo ^a   Chavez, Maria A ^a   Duarte, Carlos A ^d  

^a Universidad de La Habana   (Cuba)

^b INSTITUTO DE CIBERNÉTICA   (Cuba)

^c UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

^d CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

HIV 1 protease; Immobilized substrate; Solid phase assay

Indexed keywords

ENZYMES; MONOCLONAL ANTIBODIES; REACTION KINETICS;

C-TERMINAL SEGMENTS; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH THROUGHPUT SCREENING; HIV-1 PROTEASE; HIV-PROTEASE INHIBITORS; PROTEASE ACTIVITIES; QUANTITATIVE DETERMINATIONS; SOLID-PHASE;

PEPTIDES;

IMMOBILIZED ENZYME; INDINAVIR; MONOCLONAL ANTIBODY; PEPTIDE; PROTEIN DU1; PROTEINASE; PROTEINASE INHIBITOR; STREPTAVIDIN; UNCLASSIFIED DRUG; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN IMMUNODEFICIENCY VIRUS 1; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; SOLID PHASE EXTRACTION; ENZYME IMMUNOASSAY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOLOGY;

HUMAN IMMUNODEFICIENCY VIRUS 1;

BIOTINYLATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HIV ANTIBODIES; HIV PROTEASE; HIV PROTEASE INHIBITORS; HIV-1; HUMANS; IMMUNOENZYME TECHNIQUES;

EID: 0036667906     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00009-X     Document Type: Article

References (19)

1. Inhibitors of the VIH-1 protease: A major success of structure-assisted drug design
2. HIV chemotherapy
3. Development of drug resistance to HIV-1 protease inhibitors
4. Assay methods for retroviral proteases
5. Sensitive, soluble chromogenic substrates for the HIV-1 proteinase
6. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer
7. A heterologous substrate assay for the HIV-1 protease engineered in Echerichia coli
8. A microtiter colorimetric assay for the HIV-1 protease
9. Assay of HIV-1 protease by use of crude preparation of enzyme and biotinylated substrate
10. Development and standardization of an immuno-quantified solid phase assay for HIV-1 aspartyl protease activity and its application to the valuation of inhibitors
11. A high throughput assay for inhibitors of HIV-1 protease. Screening of microbial metabolites
12. An assay for detecting nanograms levels of proteolytic enzymes
13. Human immunodeficiency virus has an aspartic-type protease that can be inhibited by pepstatin A
14. Kinetics of antigen antibody reactions at solid-liquid interfaces
15. Extending the quasi-steady state approximation by changing variables
16. Kinetics analysis of the progress curves
17. Development of a 96-well enzyme-linked solid-phase assay for beta-glucanase and xylanase
18. Enzyme-linked immunosorbent assay for measurement of JNK, ERK, and p38 kinase activities
19. Theoretical and practical aspects of proteinase inhibition experiments