Reduction of α-galactosyl xenoantigen by expression of endo-β-galactosidase C in pig endothelial cells

Xenotransplantation

Volumn 9, Issue 4, 2002, Pages 290-296

  Ogawa, Haruko ^a   Kobayashi, Takaaki ^a   Yokoyama, Itsuo ^a   Nagatani, Naoki ^a   Mizuno, Masaaki ^a   Yoshida, Jun ^a   Kadomatsu, Kenji ^a   Muramatsu, Hisako ^a   Nakao, Akimasa ^a   Muramatsu, Takashi ^a  

^a NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

galactosyl epitope; 1,3 galactosyltransferase; Endo galactosidase C; Gene expression; Trans Golgi network; Xenotransplantation

Indexed keywords

ALPHA 1,3 GALACTOSYLTRANSFERASE; ALPHA GALACTOSYL XENOANTIGEN; ANTIGEN; BETA GALACTOSIDASE; ENDO BETA GALACTOSIDASE C; EPITOPE; GALACTOSYLTRANSFERASE; IMMUNOGLOBULIN KAPPA CHAIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL LYSATE; CELL STRAIN COS7; CELL SURFACE; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVITY; ENZYME LOCALIZATION; ERYTHROCYTE; GENE EXPRESSION; GENE LOCATION; GENE SEQUENCE; GENE TRANSFER; GENETIC TRANSFECTION; GOLGI COMPLEX; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SWINE; TRANSGENIC ANIMAL; VASCULAR ENDOTHELIUM; XENOTRANSPLANTATION;

ANIMALS; ANTIGENS, HETEROPHILE; BASE SEQUENCE; BETA-GALACTOSIDASE; CARBOHYDRATE SEQUENCE; CERCOPITHECUS AETHIOPS; COS CELLS; DISACCHARIDES; DNA PRIMERS; ENDOTHELIUM, VASCULAR; EPITOPES; GLYCOSIDE HYDROLASES; KIDNEY; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SWINE; TRANSFECTION; TRISACCHARIDES;

EID: 0036665034     PISSN: 0908665X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3089.2002.01098.x     Document Type: Article

References (14)

1. KOBAYASHI T, COOPER DKC. Anti-gal, α-gal epitopes, and xenotransplantation. Subcell Biochem 1999; 32: 229.
2. PARKER W, LIN SS, PLATT JL. Antigen expression in xenotransplantation: how low must it go? Transplantation 2001; 71: 313.
3. FUSHUKU N, MURAMATSU H, UEZONO MM, MURAMATSU T. A new endo-β-galactosidase releasing Galα1-3Gal from carbohydrate moieties of glycoproteins and from a glycolipid. J Biol Chem 1987; 262: 10086.
4. OGAWA H, MURAMATSU H, KOBAYASHI T et al. Molecular cloning of Endo-β-galactosidase C and its application in removing α-galactosyl xenoantigen from blood vessels in the pig kidney. J Biol Chem 2000; 275: 19368.
5. LIU D, KOBAYASHI T, YOKOYAMA I et al. Enzymatic removal of αGal antigen in pig kidneys by ex vivo and in vivo administration of endo-β-galactosidase C. Xenotransplantation 2002; 9: 228.
6. KATAYAMA A, OGAWA H, KADOMATSU K et al. Porcine α-1,3-galactosyltransferase: full length cDNA cloning, genomic organization, and analysis of splicing variants. Glycoconjugate J 1998; 15: 583.
7. SANGER F, NICKLEN S, COULSON AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977; 74: 5463.
8. NIWA H, YAMAMURA K, MIYAZAKI J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 1991; 108: 193.
9. MIZUNO M, YOSHIDA J, SUGITA K et al. Growth inhibition of glioma cells transfected with the human β-interferon gene by liposomes coupled with a monoclonal antibody. Cancer Res 1990; 50: 7826.
10. WATIER H, GUILLAUMIN JM, PILLER F et al. Removal of terminal α-galactosyl residues from xenogeneic porcine endothelial cells. Decrease in complement-mediated cytotoxicity but persistence of IgG1-mediated antibody dependent cell-mediated cytotoxicity. Transplantation 1996; 62: 105.
11. SANDRIN MS, DABKOWSKI PL, HENNING MM et al. Characterization of cDNA clones for porcine α(1,3) galactosyl transferase: the enzyme generating the Galα(1,3) Gal epitope. Xenotransplantation 1994; 1: 81.
12. HENION TR, MACHER BA, ANARAKI F, GALILI U. Defining the minimal size of catalytically active primate α1,3 galactosyltransferase: structure-function studies on the recombinant truncated enzyme. Glycobiology 1994; 4: 193.
13. MASIBAY AS, BALAJI PV, BOEGGEMAN EE, QASBA PK. Mutational analysis of the golgi retention signal of bovine β-1,4-galactosyltransferase. J Biol Chem 1993; 268: 9908.
14. MILLAND J, TAYLOR SG, DODSON HC, McKENZIE IFC, SANDRIN MS. The cytoplasmic tail of α1,2-fucosyltransferase contains a sequence for golgi localization. J Biol Chem 2001; 276: 12012.