Human platelet nitric oxide synthase activity: An optimized method

Revista Brasileira de Ciencias Farmaceuticas/Brazilian Journal of Pharmaceutical Sciences

Volumn 38, Issue 3, 2002, Pages 305-313

  Kawamato, Elisa Mitiko ^a   Glezer, Isaias ^a   Munhoz, Carolina Demarchi ^a   Bernardes, Cristiane ^a   Scavone, Cristoforo ^a   Marcourakis, Tania ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Arginine; Citrulline; Nitric Oxide Synthase; Platelets

Indexed keywords

ARGININE; CITRULLINE; EGTAZIC ACID; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; N(G) NITROARGININE METHYL ESTER; NITRIC OXIDE SYNTHASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TETRAHYDROBIOPTERIN; TRIFLUOPERAZINE;

ARTICLE; CONTROLLED STUDY; ENDOTHELIUM; ENZYME ACTIVITY; ENZYME INHIBITION; HUMAN; THROMBOCYTE;

EID: 0036664328     PISSN: 15169332     EISSN: None     Source Type: Journal    
DOI: 10.1590/S1516-93322002000300006     Document Type: Article

References (33)

1. γ-methylarginine, an inhibitor of endothelium derives nitric oxide synthesis, is a potent pressor agent in the guinea-pig: Does nitric oxide synthesis regulate blood pressure in vivo ? Biochem. Biophys. Res. Commun., v. 160, p. 881-886, 1989.
2. Barjavel, M. J., Bhargava, H. N. Nitric oxide synthase activity in brain regions and spinal cord of mice and rats: Kinetic analysis. Pharmacology, v. 50, p. 168-174, 1995.
3. Berkels, R., Stockklauser, K., Rosen, P., Rosen, R. Current status of platelet NO synthases. Thromb. Res., v. 87, p. 51-55, 1997.
4. Bradford, M. M. A rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., v. 72, p. 278, 1976.
5. Bredt, D. S., Snyder, S. H. Isolation of nitric oxide synthase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. USA, v. 87, p. 682-685, 1990.
6. Camacho, A., Dimsdale, J. E. Platelets and psychiatry: Lessons learned from old and new studies. Psychosomatic Med., v. 62, p. 326-336, 2000.
7. Cerchiaro, G. A., Scavone, C., Teixeira, S., Sannomiya, P. Inducible nitric oxide synthase in rat polymorphonuclear leukocytes: Role of insulin. Biochem. Pharmacol., v. 62, p. 357-362, 2001.
8. Chen, L. Y., Mehta, J. L. Variable effects of L-arginine analogs on L-arginine- nitric oxide pathway in human neutrophils and platelets may relate to different nitric oxide synthase idoforms. J. Pharmacol. Exp. Ther., v. 276, p. 253-257, 1996.
9. Chiavegatto, S., Scavone-e, C., Canteras, N. S. Nitric oxide synthase activity in the dorsal periaqueductal gray of rats expressing innate fear response. NeuroReport, v. 9, p. 571-576, 1998.
10. Cho, H. J., Xie, Q. W., Calaycay, J., Mumford, R. A., Swiderek, K. M., Lee, T. D., Nathan, C. Calmodulin is a subunit of nitric oxide synthase from macrophages. J. Exp. Med., v. 176, p. 599-604, 1992.
11. Conrad, K. P., Davis, A. K. Nitric oxide synthase activity in placentae from women with pre-eclampsia. Placenta, v. 16, p. 691-699, 1995.
12. Garthwaite, J., Boulton, C. L. Nitric oxide signaling in the central nervous system. Ann. Rev. Physiol., v. 57, p. 683-706, 1995.
13. Garvey, P., Tuttle, J. V., Covington, K., Merrill, B. M., Wood, E. R., Baylis, S. A., Charles, I. G. Purification and characterization of the constitutive nitric oxide synthase from human placenta. Arch. Biochem. Biophys., v. 311, p. 235-241, 1994.
14. Gasparini, L., Racchi, M., Binetti, C., Trabucchi, M., Solerte, S. B., Alkon, D., Etcheberrigaray, R., Gibson, G., Paoletti, R., Govoni, S. Peripheral markers in testing pathophysiological hypothesis and diagnosing Alzheimer's disease. FASEB J., v. 12, p. 17-34, 1998.
15. Hibbs, J. B., Taintor, R. R., Vavrin, Z., Granger, D. L., Drapier, J. C., Amber, I. J., Lancaster, J. R. Synthesis of nitric oxide from a terminal guanidino atom of L-arginine: A molecular mechanism regulating cellular proliferation that targets intracellular iron. In: Moncada, S., Higgs, E. A., eds. Nitric Oxide from L-arginine: A Bioregulatory System. Amsterdam: Elsevier, 1990. p. 189-223.
16. Hevel, J. M., Marletta, M. A. Nitric-oxide synthase assays. Methods Enzymol., v. 233, p. 250-258, 1994.
17. Hourani, S. M., Cusack, N. J. Pharmacological receptors on blood platelets. Pharmacol. Rev., v. 43, p. 243-298, 1991.
18. 2+ concentration. Biochem. Biophys. Res. Commun., v. 215, p. 842-848, 1995.
19. Lincoln, J., Messersmith, W. A. Conditions required for the measurement of nitric oxide synthase activity in a myenteric plexus/smooth muscle preparation from the rat ileum. J. Neurosci. Methods, v. 59, p. 191-197,1995.
20. Malinski, T., Radomski, M. W., Taha, Z., Moncada, S. Direct eletrochemical measurement of nitric oxide released from human platelets. Biochem. Biophys. Res. Commun., v. 194, p. 960-965, 1993.
21. Mckee, M., Scavone, C., Nathanson, J. A. Nitric oxide, cGMP, and hormone regulation of active sodium transport. Proc. Natl. Acad. Sci., v. 91, p. 12056-12060, 1994.
22. Mehta, J. L., Chen, L. Y., Mehta, K. P., Turner, P. Identification of constitutive and inducible forms of nitric oxide synthase in human platelets. J. Lab. Clin. Med., v. 125, p. 370-377, 1995.
23. Miles, A. M., Bohle, D. S., Glassbrenner, B., Hansert, D. A., Wink, G., Grisham, M. B. Modulation of superoxide-dependent oxidation and hydroxylation reactions by nitric oxide. J. Biol. Chem., v. 271, p. 40-47, 1996.
24. Muruganandam, A., Mutus, B. Isolation of nitric oxide synthase from human platelets. Biochim. Biophys. Acta, v. 1200, p. 1-6, 1994.
25. Pletscher, A., Shore, P. A., Brodie, B. B. Serotonin as a mediator of reserpine action in brain. J. Pharmacol. Exp. Ther., v. 116, p. 84-89, 1956.
26. Pletscher, A. Platelets as models: Use and limitations. Experientia, v. 44, p. 152-155, 1988.
27. Pontieri, V., Venezuela, M. K., Scavone, C., Michelini, L. C. Role of endogenous nitric oxide in the nucleus tractus solitarii on baroreflex control of heart rate in spontaneously hypertensive rats. J. Hypertens., v. 16, p. 1993-1999, 1998.
28. Radomski, M. W., Palmer, R. M. J., Moncada, S. Characterization of the L-arginine: Nitric oxide pathway in human platelets. Brit. J. Pharmacol., v. 101, p. 325-328, 1990a.
29. Radomski, M. W., Palmer, R. M. J., Moncada, S. An L-arginine/nitric oxide pathway present in human platelets regulates aggregation. Proc. Natl. Acad. Sci., v. 87, p. 5193-5197, 1990b.
30. Rees, D. D., Palmer, R. M. J., Hodson, H. F., Moncada, S. A specific inhibitor of nitric oxide formation from L-arginine attenuates endothelium dependent relaxation. Brit. J. Pharmacol., v. 96, p. 418-424, 1989.
31. Riddell, D. R., Graham, A., Owen, J. S. Apolipoprotein E inhibits platelet aggregation through the L-arginine:nitric oxide pathway. J. Biol. Chem., v. 272, p 89-95, 1997.
32. Sase, K., Michel, T. Expression of constitutive endothelial nitric oxide synthase in human blood platelets. Life Sci., v. 57, p. 2049-2055, 1995.
33. Wolf, D. J., Lubeskie, A., Umansky, S. The inhibition of the constitutive bovine endothelial nitric oxide synthase by imidazole and indazole agents. Arch. Biochem. Biophys., v. 314, p. 360-366, 1994.