Phenotype of hepatocyte spheroids in Arg-Gly-Asp (RGD) containing a thermo-reversible extracellular matrix

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 7, 2002, Pages 1473-1478

  Park, Keun Hong ^a   Bae, You Han ^b  

^a Seoul National University   (South Korea)

^b Gwangju Institute of Science and Technology (GIST)   (South Korea)

Author keywords

Artificial liver; Hepatocytes; Spheroids; Thermo reversible extracellular matrix

Indexed keywords

MAMMALIA;

ACRYLIC ACID RESIN; ARGINYL-GLYCYL-ASPARTIC ACID; ARGINYLGLYCYLASPARTIC ACID; CELL ADHESION MOLECULE; LIGAND; OLIGOPEPTIDE; POLY(N ISOPROPYLACRYLAMIDE CO ACRYLAMIDE); POLY(N-ISOPROPYLACRYLAMIDE-CO-ACRYLAMIDE); UREA;

ANIMAL; ARTICLE; CELL SEPARATION; CELL SURVIVAL; EXTRACELLULAR MATRIX; GEL; IN VITRO STUDY; LIVER; LIVER CELL; METABOLISM; MOLECULAR WEIGHT; MULTICELLULAR SPHEROID; PHENOTYPE; PHYSIOLOGY; RAT; SPRAGUE DAWLEY RAT; TEMPERATURE;

ACRYLIC RESINS; ANIMALS; CELL ADHESION MOLECULES; CELL SEPARATION; CELL SURVIVAL; EXTRACELLULAR MATRIX; GELS; HEPATOCYTES; LIGANDS; LIVER; MOLECULAR WEIGHT; OLIGOPEPTIDES; PHENOTYPE; RATS; RATS, SPRAGUE-DAWLEY; SPHEROIDS, CELLULAR; TEMPERATURE; UREA;

EID: 0036653709     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.1473     Document Type: Article

References (17)

1. Demitrious, A. A., Levenson, S. M., Novikoff, P. M., Novikoff, A. B., and Chowdhury A. R., Survival, organization, and function of microcarrier-attached hepatocytes transplanted in rats. Proc. Natl. Acad. Sci., USA, 83, 7475-7479 (1986).
2. Hoofnagle, J. H., Carithers, R. L. Jr., Shapiro, C., and Ascher N., Fulmination hepatic failure: Summary of a workshop. Hepatology, 21, 240-252 (1995).
3. Jauregui, H. O., Mullon, C. J., Trenkler, D., Naik, S., and Santangini, H., In vivo evaluation of a hollow fiber liver assist device. Hepatology, 21, 460-469 (1995).
4. Woerly, S., Marchand, R., and Lavallee, A., Inter-acerebral implantation of synthetic polymer/biopolymer matrix: A new perspective for brain repair. Biomaterials, 11, 97-107 (1990).
5. King, G., and Ashby, M., Cellular solids: Structure and properties. Pergamon Press, New York, U.S.A. (1988).
6. Koide, N., Sakaguchi, K., Koide, Y., Asano, K., Kawaguchi, M., Matsushima, H., Takenami, T., Shinji, U., Mori, M., and Tsuji, T., Formation of multicellular spheroids composed of adult rat hepato-cytes in dishes with positively charged and under other nonadherent environments. Exp. Cell Res., 186, 227-235 (1990).
7. Landry, J., Bernier, D., Ouellet, C., Goyette, R., and Marceau, N., Spheroidal aggregate culture of rat liver cells: Histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J. Cell Biol., 101, 914-923 (1985).
8. Koide, N., Shinji, T., Tanabe, T., Asano, K., Kawaguchi, M., Sakaguchi, K., Koide, Y., Mori, M., and Tsuji, T., Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver derived proteoglycans. Biochem. Biophys. Res. Comm., 161, 385-391 (1989).
9. Tong, J. Z., Bernard, O., and Alvarez, F., Long-term culture of rat liver cell spheroids in hormonally defined media. Exp. Cell Res., 189, 87-92 (1990).
10. Asano, K., Koide, N., and Tsuji, T., Ultrastructure of multicellular spheroid formed in primary culture of adult rat hepatocytes. J. Clin. Electron Microsc., 22, 243-252 (1989).
11. Lazar, A., Mann, H. J., Remmel, R. P., Shatford, R. A., Cerra, F. B., and Hu, W. S., Formation of porcine hepatocyte spheroids for use in a bioartificial liver. Cell Transplant., 4, 259-268 (1995).
12. Park, K.-H., Park, J. Y., and Bae, Y. H., Phenotype of hepatocyte spheroids in synthetic thermo-reversible extracellular matrix. Biotech. Bioprocess Eng., 6, 1-6 (2001)
13. Seglen, P. O., Preparation of isolated rat liver cells. Methods cell Biol., 13, 29-83 (1976).
14. Nyberg, S. L., Shatford, R. A., Payne, W. D., Hu, W. S., and Cerra, F. B., Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver. Cytotechnology, 10, 205-215 (1992).
15. Shiraha, H., Koide, N., Hada, H., Ujike, K., Nakamura, M., Shinji, T., Gotoh, S., and Tsuji, T., Improvement of serum amino acid profile in hepatic failure with a bioartificial liver using multicellular hepatocyte spheroids. Biotechnology and Bioengineering, 50, 416-421 (1996).
16. Chaney, A. L. and Marback, E. P., Modified reagents for determination of urea and ammonia. Clin. Chem., 8, 130-132 (1962).
17. Nakamura, T., Yoshimoto, K., and Nakayama, Y., Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture cell-cell contact and cell membrane. Proc. Natl. Acad. Sci., USA, 80, 7229-7233 (1983).