Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

Mechanisms of Ageing and Development

Volumn 123, Issue 10, 2002, Pages 1415-1418

  Lacelle, Chantale ^a,b   Wang, Eugenia ^b  

^a MCGILL UNIVERSITY   (Canada)

^b University of Louisville School of Medicine   (United States)

Author keywords

Cell lines; EBV; Extremely old; Longevity

Indexed keywords

ADULT; AGED; ARTICLE; B LYMPHOCYTE; BLOOD SAMPLING; CONTROLLED STUDY; EPSTEIN BARR VIRUS; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL LINE; NONHUMAN; PRIORITY JOURNAL; SENESCENCE;

NON-PROGRAMMATIC;

ADULT; AGED; AGED, 80 AND OVER; B-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELLS, CULTURED; HUMANS; LEUKOCYTES, MONONUCLEAR; MIDDLE AGED; PLASMA;

HUMAN HERPESVIRUS 4;

EID: 0036629915     PISSN: 00476374     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0047-6374(02)00074-X     Document Type: Article

References (6)

1. Neitzel H. A routine method for the establishment of permanent growing lymphoblastoid cell lines. Hum. Genet. 73:1986;320-326.
2. Sugden B., Mark W. Clonal transformation of adult human leukocytes by Epstein-Barr virus. J. Virol. 23:1977;503-508.
3. Thornley-Lawson D.A., Chess L., Strominger J.L. Suppression of in vitro Epstein-Barr virus infection: a new role for adult human T lymphocytes. J. Exp. Med. 146:1977;495-508.
4. Tosato, G., Pikes, S.E., Koski, I., Blaese, R.M. 1982. Selective inhibition of immunoregulatory cell functions by cyclosporine A. J. Immunol. 1982.
5. Tosato G. Generation of epstein-barr virus (EBV) immortalized cell lines. Current protocols in immunology. Curr. Protocol Unit. 7:1991;22.
6. Tremblay S., Khandjian E. Successful use of long-term frozen lymphocytes for the establishment of lymphoblastoid cell lines. Clin. Biochem. 31:1998;555-556.