Long wavelength fluorophores and cell-by-cell correction for autofluorescence significantly improves the accuracy of flow cytometric energy transfer measurements on a dual-laser benchtop flow cytometer

Cytometry

Volumn 48, Issue 3, 2002, Pages 124-135

  Sebestyén, Zsolt ^a   Nagy, Péter ^b   Horváth, Gábor ^a   Vámosi, György ^b   Debets, Reno ^c   Gratama, Jan W ^c   Alexander, Denis R ^d   Szöllosi, János ^a  

^a UNIVERSITY OF DEBRECEN   (Hungary)

^b UNIVERSITY OF DEBRECEN   (Hungary)

^c ERASMUS MEDICAL CENTER   (Netherlands)

^d BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

Autofluorescence; CD45 isoforms; Cyanine 3 and 5 donor acceptor pair; Flow cytometric resonance energy transfer (FCET); HLA class I molecules; Indirect immunofluorescence

Indexed keywords

ANTIBODY; CD45 ANTIGEN; CYANINE DYE; FLUORESCENT DYE;

ACCURACY; ALGORITHM; ANTIBODY LABELING; ARTICLE; AUTOANALYZER; CELL LABELING; ENERGY TRANSFER; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SIGNAL NOISE RATIO; CALIBRATION; CELL CULTURE; CELL LINE; COMPARATIVE STUDY; EVALUATION; IMAGE SUBTRACTION; INSTRUMENTATION; LASER; METHODOLOGY; REPRODUCIBILITY; SPECTROFLUOROMETRY;

ALGORITHMS; CALIBRATION; CELL LINE, TRANSFORMED; COMPARATIVE STUDY; ENERGY TRANSFER; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; HUMAN; LASERS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE; SUBTRACTION TECHNIQUE; SUPPORT, NON-U.S. GOV'T; TUMOR CELLS, CULTURED; HUMANS;

EID: 0036628631     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.10121     Document Type: Article

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