Allele-specific PCR amplification due to sequence identity between a PCR primer and an amplicon: Is direct sequencing so reliable?

Human Genetics

Volumn 110, Issue 6, 2002, Pages 606-608

  Ikegawa, Shiro ^a,b   Mabuchi, Akihiko ^b   Ogawa, Masako ^b   Ikeda, Toshiyuki ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

5' UNTRANSLATED REGION; ALLELE; AMPLICON; ARTICLE; BASE PAIRING; COMPARATIVE STUDY; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; GENE MUTATION; GENE SEQUENCE; GENOTYPE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; NORMAL HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; SINGLE STRAND CONFORMATION POLYMORPHISM;

ALLELES; BASE SEQUENCE; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; DNA-BINDING PROTEINS; GENOTYPE; HUMANS; PAX9 TRANSCRIPTION FACTOR; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSCRIPTION FACTORS;

EID: 0036626516     PISSN: 03406717     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00439-002-0735-1     Document Type: Article

References (2)

1. Grompe M (1993) The rapid detection of unknown mutations in nucleic acids. Nat Genet 5:111-117
2. Holland PM, Abramson RD, Watson R, Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 88:7276-7280