Simultaneous estimation of intracellular free Mg2+ and pH by use of a new pH-dependent dissociation constant of MgATP

Japanese Journal of Physiology

Volumn 52, Issue 3, 2002, Pages 323-326

  Nakayama, Shinsuke ^a   Nomura, Hideki ^a   Smith, Lorraine M ^a   Clark, Joseph F ^b  

^a NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF CINCINNATI MEDICAL CENTER   (United States)

Author keywords

Magnesium; pH; Smooth muscle

Indexed keywords

ADENOSINE TRIPHOSPHATE MAGNESIUM; MAGNESIUM; SODIUM;

ARTICLE; CALCULATION; CELL LEVEL; CELL PH; DISSOCIATION CONSTANT; METHODOLOGY; SMOOTH MUSCLE; TRANSPORT KINETICS;

ADENOSINE TRIPHOSPHATE; ANIMALS; CECUM; GUINEA PIGS; HYDROGEN; HYDROGEN-ION CONCENTRATION; INTRACELLULAR MEMBRANES; MAGNESIUM; MODELS, BIOLOGICAL; SODIUM;

EID: 0036620725     PISSN: 0021521X     EISSN: None     Source Type: Journal    
DOI: 10.2170/jjphysiol.52.323     Document Type: Article

References (12)

1. 31P nuclear magnetic resonance spectroscopy. In: Biochemistry of Smooth Muscle Contraction, ed. Bárány M, Academic Press, San Diego, pp 393-404, 1996
2. 2+ in intact frog skeletal muscle. J Biol Chem 255: 3987-3993, 1981
3. Aickin CC: Movement of acid equivalents across the mammalian smooth muscle cell membrane. In: Proton Passage Across Cell Membrane, Ciba Foundation Symposium 139, ed. Bock G and Marsh J, John Wiley & Sons Ltd., Chichester, pp 3-22, 1988
4. Wray S: Smooth muscle intracellular pH: measurement, regulation, and function. Am J Physiol 254: C213-C225, 1988
5. 31P nuclear magnetic resonance. Blood 65: 1526-1530, 1985
6. Nakayama S, Nomura H, and Tomita T: Intracellular-free magnesium in the smooth muscle of guinea pig taenia caeci: a concomitant analysis for magnesium and pH upon sodium removal. J Gen Physiol 103: 833-851, 1994
7. 31P-NMR ionized magnesium determinations. Anal Biochem 251: 246-250, 1997
8. 2+-free solution in the taenia isolated from guinea-pig caecum. J Physiol (Lond) 421: 363-378, 1990
9. Nakayama S and Tomita T: Regulation of intracellular free magnesium concentration in the taenia of guinea pig caecum. J Physiol (Lond) 435: 559-572, 1991
10. Nakayama S and Clark JF: Smooth muscle and NMR review: an overview of smooth muscle metabolism. Mol Cell Biochem (in press)
11. 2+ concentration in smooth muscle cells of guinea-pig tenia cecum: intracellular calibration of the fluorescent indicator fraptra. Biophys J 73: 3358-3370, 1997
12. 2+ transport in vascular smooth muscle cells in genetic hypertension. Hypertension 34: 442-449, 1999