Red food coloring stain: New, safer procedures for staining nematodes in roots and egg masses on root surfaces

Journal of Nematology

Volumn 34, Issue 2, 2002, Pages 179-181

  Thies, Judy A ^a   Merrill, Sharon B ^a   Corley, E Luther ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Environmental safety; Meloidogyne spp.; Resistance; Root knot nematode; Staining procedures; Worker protection

Indexed keywords

EID: 0036619452     PISSN: 0022300X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Byrd, D. W. Jr., T. Kirkpatrick, and K. R. Barker. 1983. An improved technique for clearing and staining tissues for detection of nematodes. Journal of Nematology 15:142-143.
2. Fenner, L. M. 1962. Determination of nematode mortality. Plant Disease Reporter 46:383.
3. Holbrook, C. C., D. A. Knauft, and D. W. Dickson. 1983. A technique for screening peanut for resistance to Meloidogyne arenaria. Plant Disease 57:957-958.
4. McBeth, C. W., A. L. Taylor, and A. L. Smith. 1941. Note on staining nematodes in root tissues. Proceedings of the Helminthological Society of Washington 16:3-6.
5. Sigma-Aldrich. 1999a. Product 198277 phloxine B, certified, material safety data sheet. St. Louis, MO.
6. Sigma-Aldrich. 1999b. Product 332704 acid fuchsin, sodium sait (70%), material safety data sheet. St. Louis, MO.
7. Southards, C. J. 1965. Host-parasite relations of the lesion nematodes, Pratylenchus brachyurus, P. zeae, and P. scribneri, and flue-cured tobacco. Ph.D. dissertation, North Carolina State University, Raleigh, NC.
8. Southey, J. F. 1970. Laboratory methods for work with plant and soil nematodes. Technical Bulletin of the Ministry of Agriculture, Fisheries & Food No. 2. 5th ed. London: Her Majesty's Stationery Office.