Freezing and thawing of bone marrow-derived murine dendritic cells with subsequent retention of immunophenotype and of antigen processing and presentation characteristics

Journal of Immunological Methods

Volumn 264, Issue 1-2, 2002, Pages 153-162

  Sai, Tao ^a   Milling, Simon W F ^a   Mintz, Beatrice ^a  

^a FOX CHASE CANCER CENTER   (United States)

Author keywords

Cryopreservation; Dendritic cells; Immunotherapy

Indexed keywords

CD11B ANTIGEN; CD40 ANTIGEN; GLYCOPROTEIN P 15095; LIPOPOLYSACCHARIDE; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; OVALBUMIN; PEPTIDE;

ANIMAL CELL; ANTIGEN PRESENTATION; ARTICLE; BONE MARROW; BONE MARROW CELL; CELL LINEAGE; CONTROLLED STUDY; CULTURE MEDIUM; DENATURATION; DENDRITIC CELL; FREEZING; GENETIC VARIABILITY; HYBRIDOMA; IMMUNOPHENOTYPING; IMMUNOTHERAPY; INBREEDING; MAJOR HISTOCOMPATIBILITY COMPLEX; MORPHOLOGY; MOUSE; NONHUMAN; PRECURSOR; PRIORITY JOURNAL; PROTEIN EXPRESSION; REDUCTION; T LYMPHOCYTE; THAWING;

ANIMALS; ANTIGEN PRESENTATION; BONE MARROW CELLS; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CELL SURVIVAL; CELLS, CULTURED; CULTURE MEDIA; DENDRITIC CELLS; EPITOPES, T-LYMPHOCYTE; FEMALE; FETAL BLOOD; FREEZING; HYBRIDOMAS; IMMUNOPHENOTYPING; INJECTIONS, INTRAVENOUS; LYMPHOCYTE CULTURE TEST, MIXED; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; OVALBUMIN; PROTEIN DENATURATION; SPLEEN; T-LYMPHOCYTE SUBSETS;

MURINAE;

EID: 0036606410     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00100-X     Document Type: Article

References (21)

1. Migration patterns of dendritic cells in the mouse. Homing to T cell-dependent areas of spleen, and binding within marginal zone
2. Induction of tumor immunity and cytotoxic T lymphocyte responses using dendritic cells transfected with messenger RNA amplified from tumor cells
3. Regulation of dendritic cell numbers and maturation by lipopolysaccharide in vivo
4. A method for the production of cryopreserved aliquots of antigen-preloaded, mature dendritic cells ready for clinical use
5. In vivo maturation and migration of dendritic cells
6. Dendritic cells in cancer immunotherapy
7. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor
8. A phagosome-to-cytosol pathway for exogenous antigens presented on MHC class I molecules
9. Equipotent generation of protective antitumor immunity by various methods of dendritic cell loading with whole cell tumor antigens
10. Freezing of dendritic cells, generated from cryopreserved leukaphereses, does not influence their ability to induce antigen-specific immune responses or functionally react to maturation stimuli
11. Dendritic cell-based immunotherapy of prostate cancer: Immune monitoring of a phase II clinical trial
12. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow
13. Presence of IgE antibodies to bovine serum albumin in a patient developing anaphylaxis after vaccination with human peptide-pulsed dendritic cells
14. Vaccination of melanoma patients with peptide- or tumor lysate-pulsed dendritic cells
15. Dendritic cells: On the move from bench to bedside
16. Immunotherapy with dendritic cells and tumor major histocompatibility complex class I-derived peptides requires a high density of antigen on tumor cells
17. Presentation of exogenous antigen with class I major histocompatibility complex molecules
18. Exact prediction of a natural T cell epitope
19. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha
20. Immunotherapy of hormone-refractory prostate cancer with antigen-loaded dendritic cells
21. Therapy of murine tumors with tumor peptide-pulsed dendritic cells: Dependence on T cells, B7 costimulation, and T helper cell 1-associated cytokines