A simple method to detect and differentiate Burkholderia pseudomallei and Burkholderia thailandensis using specific flagellin gene primers

Molecular and Cellular Probes

Volumn 16, Issue 3, 2002, Pages 217-222

  Sonthayanon, Piengchan ^a   Krasao, Piamnukul ^a   Wuthiekanun, Vannaporn ^a   Panyim, Sakol ^a   Tungpradabkul, Sumalee ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Burkholderia pseudomallei; Burkholderia thailandensis; Flagellin sequence; Polymerase chain reaction (PCR)

Indexed keywords

FLAGELLIN; BACTERIAL DNA; PRIMER DNA;

ANALYTIC METHOD; ARTICLE; BACTERIUM DETECTION; BURKHOLDERIA; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS; CONTROLLED STUDY; GENETIC ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SOIL; BACTERIAL INFECTION; CLASSIFICATION; GENETICS; HUMAN; MICROBIOLOGY; SOUTHEAST ASIA; STANDARD;

BURKHOLDERIA; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS;

ASIA, SOUTHEASTERN; BURKHOLDERIA; BURKHOLDERIA INFECTIONS; BURKHOLDERIA PSEUDOMALLEI; DNA PRIMERS; DNA, BACTERIAL; FLAGELLIN; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY;

EID: 0036599655     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.2002.0413     Document Type: Article

References (12)

1. Biochemical characteristics of clinical and environmental isolates of Burkholderia pseudomallei
2. Arabinose assimilation defines a nonvirulent biotype of Burkholderia pseudomallei
3. Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species
4. Stable marker on flagellin gene sequences related to arabinose non-assimilating pathogenic Burkholderia pseudomallei
5. Quantitative recovery of Burkholderia pseudomallei from soil in Thailand
6. Simultaneous detection of microorganisms in soil suspension based on PCR amplification of bacterial 16S rRNA fragments
7. Relief of amplification in PCR with bovine serum albumin or T4 Gene32 protein
8. Detection of bacterial virulence genes by subtractive hybridization: Identification of capsular polysaccharide of Burkholderia pseudomallei as a major virulence determinant
9. Ecology of Burkholderia pseudomallei and the interactions between environmental Burkholderia spp. and human-animal hosts
10. Evaluation of culture techniques for isolation of Pseudomonas pseudomallei from soil
11. Isolation of Pseudomonas pseudomallei from soil in northeastern Thailand
12. Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction