Site-directed mutagenesis within the central constriction site of ScrY (sucroseporin): Effect on ion transport and comparison of maltooligosaccharide binding to LamB of Escherichia coli

Journal of Membrane Biology

Volumn 187, Issue 3, 2002, Pages 239-253

  Kim, B H ^a   Andersen, C ^a   Kreth, J ^b   Ulmke, C ^b   Schmid, K ^b   Benz, R ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

Carbohydrate binding; LamB; Lipid bilayer membrane; Noise analysis; ScrY; Sucrose transport

Indexed keywords

MALTOPORIN; MALTOSE OLIGOSACCHARIDE; MEMBRANE PROTEIN; OLIGOSACCHARIDE; SUCROSEPORIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; ESCHERICHIA COLI PROTEIN; LAMBDA PHAGE RECEPTOR; MALTOOLIGOSACCHARIDES; MALTOPENTAOSE; PORIN; SCRY PROTEIN, BACTERIA; SCRY PROTEIN, E COLI; VIRUS RECEPTOR;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; CARBOHYDRATE TRANSPORT; ESCHERICHIA COLI; ION CURRENT; ION TRANSPORT; LIPID BILAYER; MEMBRANE CONDUCTANCE; NONHUMAN; SITE DIRECTED MUTAGENESIS; BINDING SITE; BIOSYNTHESIS; CARBOHYDRATE METABOLISM; CELL MEMBRANE POTENTIAL; CHEMICAL STRUCTURE; CHEMISTRY; CLASSIFICATION; COMPARATIVE STUDY; ELECTRIC CONDUCTIVITY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PROTEIN BINDING; SPECIES DIFFERENCE; STATISTICS; TRANSPORT AT THE CELLULAR LEVEL;

ESCHERICHIA COLI; LAMB; NEGIBACTERIA;

BACTERIAL PROTEINS; BINDING SITES; BIOLOGICAL TRANSPORT; CARBOHYDRATE METABOLISM; ELECTRIC CONDUCTIVITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ION TRANSPORT; MEMBRANE POTENTIALS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; PORINS; PROTEIN BINDING; RECEPTORS, VIRUS; SPECIES SPECIFICITY; STOCHASTIC PROCESSES;

EID: 0036591523     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-001-0167-1     Document Type: Article

References (39)

1. Study of sugar binding to the sucrose specific ScrY-channel of enteric bacteria using current noise analysis
2. Evaluation of the rate constants of sugar transport through maltoprin (LamB) of Escherichia coli from the sugar-induced current noise
3. Salmonella typhimurium contains an anion-selective outer membrane porin induced by phosphate starvation
4. Structure and function of porins from gram-negative bacteria
5. Permeation of hydrophilic molecules through the outer membrane of gram-negative bacteria
6. Mechanism of ion transport through the anion-selective channel of the Pseudomonas aeruginosa outer membrane
7. Formation of large, ion-permeable membrane channels by the matrix protein (porin) of Escherichia coli
8. Pore formation by Lamb of Escherichia coli in lipid bilayer membranes
9. Mechanism of sugar transport through the sugar-specific Lamb channel of Escherichia coli outer membrane
10. Ultrastructure, chemistry and function of the bacterial wall
11. Maltose-binding protein does not modulate the activity of maltoporin as a general porin in Escherichia coli
12. Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12
13. Channel noise in membranes and lipid bilayers
14. New mechanism of antibiotic resistance in Enterabacteriaceae induced by a structural modification of the major porin
15. Extended sugar slide function for the periplasmic coiled coil domain of ScrY
16. Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway
17. Lambda receptor in the outer membrane of Escherichia coli as a binding protein for maltodextrins and starch polysaccharides
18. Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucrose
19. Role of porins in outer membrane permeability
20. Outer membrane protein P of Pseudomonas aeruginosa: Regulation by phosphate deficiency and formation of small anion-specific channels in lipid bilayer membranes
21. Plasmid-mediated sucrose metabolism in Escherichia coli: Characterization of scrY, the structural gene for a phosphoenolpyruvate- dependent sucrose phosphotransferase system outer membrane porin
22. Ion transport through pores: A rate-theory analysis
23. Pore-forming activity of the Tsx protein from the outer membrane of Escherichia coli. Demonstration of a nucleoside-specific binding site
24. Noise analysis of ion current through the open and the sugar-induced closed state of the LamB-channel of Escherichia coli outer membrane: Evaluation of the sugar binding kinetics to the channel interior
25. Molecular basis of bacterial outer membrane permeability
26. Structural basis for sugar translocation maltoporin channels at 3.1 Å resolution
27. Plasmid-mediated sucrose metabolism in Escherichia coli K-12: Mapping of the scr genes of pUR400
28. A sugar-specific porin, ScrY, is involved in sucrose uptake in enteric bacteria
29. Plasmid mediated uptake and metabolism of sucrose by Escherichia coli K-12
30. The sugar specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins
31. The deletion of 70 N-terminal amino acids of the sugar-specific sucrose-porin ScrY causes its functional similarity to Lamb in vivo and in vitro
32. The maltose regulon
33. Maltose transport in Escherichia coli K-12: A comparison of transport kinetics in wildtype and lambda-resistant mutants with the dissociation constant of the maltose-binding protein as measured by fluorescence quenching
34. Outer membrane proteine of Escherichia coli K-12 is coregulated with alkaline phosphatase
35. Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity
36. Understanding the function of bacterial outer membrane channels by reconstitution into black lipid membranes
37. Membrane noise. Prog. Biophys
38. 1/f-noise of open bacterial porin channels