Characterization of Streptococcus pneumoniae thymidylate kinase: Steady-state kinetics of the forward reaction and isothermal titration calorimetry

Biochemical Journal

Volumn 363, Issue 3, 2002, Pages 825-831

  Petit, Chantal M ^a   Koretke, Kristin K ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

AZT MP; Dissociation constants; DTMP kinase; Kinetic mechanisms; Nucleotide specificity

Indexed keywords

ADENOSINETRIPHOSPHATE; CALORIMETRY; CLONING; ESCHERICHIA COLI; PH EFFECTS; REACTION KINETICS; TITRATION;

SALVAGE PATHWAYS;

BIOCHEMISTRY;

ADENOSINE TRIPHOSPHATE; CYTIDINE TRIPHOSPHATE; PHOSPHOTRANSFERASE; THYMIDINE DIPHOSPHATE; THYMIDINE PHOSPHATE; THYMIDINE TRIPHOSPHATE; THYMIDYLATE KINASE; UNCLASSIFIED DRUG; ZIDOVUDINE 5' PHOSPHATE;

ARTICLE; CALORIMETRY; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME SPECIFICITY; INHIBITION KINETICS; MOLECULAR CLONING; NONHUMAN; PH; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; CALORIMETRY; CYTIDINE TRIPHOSPHATE; DEOXYURACIL NUCLEOTIDES; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; NUCLEOSIDE-PHOSPHATE KINASE; PHOSPHORYLATION; STREPTOCOCCUS PNEUMONIAE; SUBSTRATE SPECIFICITY; THYMINE NUCLEOTIDES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE;

EID: 0036566484     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3630825     Document Type: Article

References (15)

1. Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis
2. Insights into the phosphoryltransfer mechanism of human thymidylate kinase gained from crystal structures of enzyme complexes along the reaction coordinate
3. Modifying human thymidylate kinase to potentiate azidothymidine activation
4. The bottleneck in AZT activation
5. Structure of thymidylate kinase reveals the cause behind the limiting step on AZT activation
6. Structural basis for efficient phosphorylation of 3′-azidothymidine monophosphate by Escherichia coli thymidylate kinase
7. The highly similar TMP kinases of Yersinia pestis and Escherichia coli differ markedly in their AZTMP phosphorylating activity
8. 5-(5′thymidyl)pentophosphate (TP5A) at 2.0 Å resolution: Implications for catalysis and AZT activation
9. Mouse ascites sarcoma 180 thymidylate kinase. General properties, kinetic analysis and inhibition studies
10. Kinetic and thermodynamic characterizations of yeast guanylate kinase
11. Initial velocity and equilibrium kinetics of myokinase
12. Guanylate kinases from human erythrocytes, hog brain and rat liver
13. Partial purification and characterization of thymidylate kinase from embryonic chick liver
14. Human thymidylate kinase. Purification, characterization and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemia
15. Kinetics of the interaction of monophosphates of the anti-viral nucleosides 2′-fluoro-1-beta-D-arabinofuranosylpyrimidine and (E)-5-(2-bromovinyl)-2′-deoxyuridine with thymidylate kinases from Vero cells and herpes simplex virus types 1 and 2