Threonine-124 and phenylalanine-448 in Citrobacter freundii tyrosine phenol-lyase are necessary for activity with L-tyrosine

Biochemical Journal

Volumn 363, Issue 3, 2002, Pages 745-752

  Demidkina, Tatyana V ^a   Barbolina, Maria V ^a   Faleev, Nicolai G ^a   Sundararaju, Bakthavatsalam ^a   Gollnick, Paul D ^a   Phillips, Robert S ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

elimination; Mutagenesis; Pyridoxal 5 phosphate; Stopped flow kinetics; Tryptophan indole lyase

Indexed keywords

AMINO ACIDS; BACTERIA; CRYSTAL STRUCTURE; ENZYMES; HYDROGEN BONDS; REACTION KINETICS;

REACTION SPECIFICITY;

BIOCHEMISTRY;

PHENYLALANINE; THREONINE; TYROSINE PHENOL LYASE;

ARTICLE; BINDING SITE; CITROBACTER FREUNDII; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; FLOW KINETICS; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; RESIDUE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BINDING SITES; CITROBACTER FREUNDII; ESCHERICHIA COLI; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; PYRIDOXAL PHOSPHATE; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP; THREONINE; TYROSINE; TYROSINE PHENOL-LYASE;

CITROBACTER; CITROBACTER FREUNDII; PROTEUS VULGARIS;

EID: 0036565775     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3630745     Document Type: Article

References (33)

1. Tyrosine phenol lyase. I. Purification, crystallization, and properties
2. Reactions of O-acyl-L-serines with tryptophanase, tyrosine phenol-lyase, and tryptophan synthase
3. Three-dimensional structure of tyrosine phenol-lyase
4. The crystal structure of Citrobacter freundii tyrosine phenol-lyase complexed with 3-(4′-hydroxyphenyl) propionic acid, together with site-directed mutagenesis and kinetic analysis, demonstrates that arginine-381 is required for substrate specificity
5. Crystal structure of tryptophanase
6. Mechanistic deductions from kinetic isotope effects and pH studies of pyridoxal phosphate-dependent carbon-carbon lyases: Erwinia herbicola and Citrobacter freundii tyrosine phenol-lyase
7. Mechanistic deductions from multiple kinetic and solvent isotope effects and pH studies of pyridoxal phosphate-dependent carbon-carbon lyases: Escherichia coli tryptophan indole-lyase
8. Tyrosine phenol-lyase from Citrobacter intermedius. Factors controlling substrate specificity
9. S-Aryl-L-cysteine S, S-dioxides: Design and evaluation of a new class of mechanism based inhibitors of kynureninase
10. A comprehensive set of sequence analysis programs for the VAX
11. Rapid and efficient site-specific mutagenesis without phenotypic selection
12. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA
13. Production of single-stranded plasmid DNA
14. DNA sequencing with chain-terminating inhibitors
15. Site-directed mutagenesis of histidine 343 to alanine in Citrobacter freundii tyrosine phenol-lyase: Effects on the kinetic mechanism and rate-determining step
16. Tryptophanase (Escherichia coli B)
17. An inducible tryptophan synthase in tryptophan auxotrophs of Escherichia coli
18. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
19. Elementary processes in the interaction of tyrosine phenol lyase with inhibitors and substrate
20. 6
21. Statistical analysis of enzyme kinetic data
22. Robust multicomponent analysis applied to the separation of components in a mixture of absorbing species
23. Determination of dissociation constants and specific rate constants of enzyme-substrate (or protein-ligand) interactions from rapid reaction kinetic data
24. Identity of the subunits of tyrosine phenol-lyase from Citrobacter intermedius
25. The role of glutamic acid-69 in the activation of Citrobacter freundii tyrosine phenol-lyase by monovalent cations
26. Binding of phenol and analogues to alanine complexes of tyrosine phenol-lyase from Citrobacter freundii: Implications for the mechanism of β-elimination and alanine racemization
27. Transamination catalysed by tyrosine phenol-lyase from Citrobacter intermedius
28. Evolutionary relationships among pyridoxal-5′-phosphate-dependent enzymes. Regio-specific alpha, beta and gamma families
29. Site-directed mutagenesis of tyrosine-71 to phenylalanine in Citrobacter freundii tyrosine phenol-lyase: Evidence for dual roles of tyrosine 71 as a general acid catalyst in the reaction mechanism and in cofactor binding
30. Tyrosine 70 fine-tunes the catalytic efficiency of aspartate aminotransferase
31. Substrate specificity of tyrosine-phenol-lyase. Electronic and steric control at the stage of aromatic moiety elimination
32. Formation in vitro of hybrid dimers of H463F and Y74F mutant Escherichia coli tryptophan indole-lyase rescues activity with L-tryptophan