Export of autotransported proteins proceeds through an oligomeric ring shaped by C-terminal domains

EMBO Journal

Volumn 21, Issue 9, 2002, Pages 2122-2131

  Veiga, Esteban ^a   Sugawara, Etsuko ^a   Nikaido, Hiroshi ^a   De Lorenzo, Victor ^b   Fernández, Luis Angel ^c  

^a DIVISION OF REPRODUCTIVE BIOLOGY   (United States)

^b UNIVERSITY OF LONDON   (United Kingdom)

^c CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Autotransporters; IgA protease; Neisseria gonorrhoeae; Protein secretion

Indexed keywords

IMMUNOGLOBULIN A PROTEASE; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CHANNEL GATING; CIRCULAR DICHROISM; COMPLEX FORMATION; ELECTROPHORETIC MOBILITY; NEISSERIA GONORRHOEAE; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; PROTEIN SECRETION;

BACTERIAL PROTEINS; ION CHANNELS; NEISSERIA GONORRHOEAE; PORINS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; SERINE ENDOPEPTIDASES;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA; NEISSERIA GONORRHOEAE;

EID: 0036565670     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.9.2122     Document Type: Article

References (65)

1. Crystal structure of a phage library-derived single-chain Fv fragment complexed with turkey egg-white lysozyme at 2.0 resolution
2. AIDA-I, the adhesin involved in diffuse adherence of diarrhoeagenic Escherichia coli strain 2787 (O126:H27), is synthesized via a precursor molecule
3. The biogenesis and assembly of bacterial membrane proteins
4. Formation of oligomeric rings by XcpQ and PilQ, which are involved in protein transport across the outer membrane of Pseudomonas aeruginosa
5. Heterologous protein secretion and the versatile Escherichia coil haemolysin translocator
6. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system
7. Periplasmic transit and disulfide bond formation of the autotransported Shigella protein IcsA
8. Type I secretion and multidrug efflux: Transport through the TolC channel-tunnel
9. XL1-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection
10. Type III machines of Gram-negative bacteria: Delivering the goods
11. Analysis of the PilQ secretin from Neisseria meningitidis by transmission electron microscopy reveals a 'dodecameric quaternary structure
12. Crystal structures explain functional properties of two E. coli porins
13. Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes
14. Biogenesis of the Gram-negative bacterial envelope
15. Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels
16. Formation of disulphide bonds during secretion of proteins through the periplasmic-independent type I pathway
17. Cloning and sequencing of a Bordetella pertussis serum resistance locus
18. A superfamily of proteins involved in different secretion pathways in gram-negative bacteria: Modular structure and specificity of the N-terminal domain
19. OmpT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification
20. Genetic dissection of the outer membrane secretin PulD: Are there distinct domains for multimerization, and secretion specificity?
21. Virulence functions of autotransporter proteins
22. The great escape: Structure and function of the autotransporter proteins
23. Autotransporter proteins, evolution and redefining protein secretion
24. Extracellular transport of cholera toxin B subunit using Neisseria IgA protease β-domain: Conformation-dependent outer membrane translocation
25. Selective extracellular release of cholera toxin B subunit by Escherichia coli: Dissection of Neisseria Igaβ-mediated outer membrane transport
26. Characterization of the Neisseria Igaβ-core. The essential unit for outer membrane targeting and extracellular protein secretion
27. Structure and function of bacterial outer membrane proteins: Barrels in a nutshell
28. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export
29. Comparative characterization of the iga gene encoding IgA1 protease in Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae
30. Secretion of proteins and assembly of bacterial surface organelles: Shared pathways of extracellular protein targeting
31. Characterization of the essential transport function of the AIDA-I autotransporter and evidence supporting structural predictions
32. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants
33. Porins and specific diffusion channels in bacterial outer membranes
34. Outer membrane
35. Effect on solute size on diffusion rates through the transmembrane pores of the outer membrane of Escherichia coli
36. Porin channels in Escherichia coil: Studies with liposomes reconstituted from purified proteins
37. Molecular basis of bacterial outer membrane permeability
38. Identification and characterization of porins in Pseudomonas aeruginosa
39. Secretin PulD: Association with pilot PulS, structure and ion-conducting channel formation
40. Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy
41. Mechanism of assembly of the outer membrane of Salmonella typhimurium
42. Analysis of the circular dichroism spectrum of proteins using the convex constraint algorithm: A practical guide
43. Type III export: New uses for an old pathway
44. Gene structure and extracellular secretion of Neisseria gonorrhoea IgA protease
45. Macromolecular assembly and secretion across the bacterial cell envelope: Type II protein secretion systems
46. Biology of type II secretion
47. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin: Structural similarities with the IgA protease type of exported protein
48. Protein composition of the cell wall and cytoplasmic membrane of E. coli
49. Outer membrane proteins of Escherichia coli. I. Effect of preparative conditions on the migration of protein in polyacrylamide gels
50. The C-terminal domain of the Bordetella pertussis autotransporter BrkA forms a pore in lipid bilayer membranes
51. Outer membrane of Salmonella typhimurium: Chemical analysis and freeze-fracture studied with lipopolysaccharide mutants
52. Bacterial adhesins: Common themes and variations in architecture and assembly
53. Secretion of virulence determinants by the general secretory pathway in gram-negative pathogens: An evolving story
54. Pore-forming activity of OmpA protein of Escherichia coli
55. Processing of the AIDA-I precursor: Removal Of AIDAc and evidence for the outer membrane anchoring as a β-barrel structure
56. Extracellular transport of VirG protein in Shigella
57. Structure and assembly of β-barrel membrane proteins
58. Substrate-induced assembly of a contiguous channel for protein export from E. coli: Reversible bridging of an inner-membrane translocase to an outer membrane exit pore
59. Multiple pathways allow protein secretion across the bacterial outer membrane
60. The chaperone/usher pathway: A major terminal branch of the general secretory pathway
61. The PapC usher forms an oligomeric channel: Implications for pilus biogenesis across the outer membrane
62. Engineering a mouse metallothionein on the cell surface of Ralstonia eutropha CH34 for immobilization of heavy metals in soil
63. Probing secretion and translocation of a β-autotransporter using a reporter single-chain Fv as a cognate passenger domain