Technical improvement to 2D-page of rice organelle membrane proteins

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 5, 2002, Pages 1170-1173

  Mikami, Satoshi ^a   Kishimoto, Tadashi ^a   Hori, Hidetaka ^a   Mitsui, Toshiaki ^a  

^a NIIGATA UNIVERSITY   (Japan)

Author keywords

2D PAGE; Membrane protein; NEPHGE; Oryza sativa L.

Indexed keywords

MEMBRANE PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CELL ORGANELLE; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; RICE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; MEMBRANE PROTEINS; ORGANELLES; ORYZA SATIVA; PLANT PROTEINS;

ORYZA SATIVA;

EID: 0036561023     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.1170     Document Type: Article

References (16)

1. O’Farrell, P. H., High-resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 250, 4007-4021 (1975).
2. Hirano, H., Varietal differences of leaf protein profiles in mulberry. Phytochemistry, 21, 1513-1518 (1982).
3. Bjellqvist, B., Ek, K., Righetti, P. G., Gianazza, E., Gorg, A., Westermeier, R., and Postel, W., Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications. J. Biochem. Biophys. Methods, 6, 317-339 (1982).
4. Dunbar, B. S., Kimura, H., and Timmons, T. M., Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis. Methods Enzymol., 182, 441-459 (1990).
5. Patton, W. F., Pluskal, M. G., Skea, W. M., Buecker, J. L., Lopez, M. F., Zimmermann, R., Belanger, L. M., and Hatch, P. D., Development of a dedicated two-dimensional gel electrophoresis system that provides optimal pattern reproducibility and polypeptide resolution. BioTechniques, 8, 518-527 (1990).
6. Herbert, B. R., Mollyo, M. P., Gooley, A. A., Walsh, B. J., Bryson, W. G., and Williams, K. L., Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis, 19, 845-851 (1998).
7. Komatsu, S., Kajiwara, H., and Hirano, H., A rice protein library: a data-file of rice proteins separated by two-dimensional electrophoresis. Theor. Appl. Genet., 86, 935-942 (1993).
8. Kamo, M., Kawakami, T., Miyatake, N., and Tsugita, A., Separation and characterization of Arabidopsis thaliana proteins by two-dimensional gel electrophoresis. Electrophoresis, 16, 423-430 (1995).
9. Santoni, V., Rouquie, D., Doumas, P., Mansion, M., Boutry, M., Degand, H., Dupree, P., Packman, L., Sherrier, J., Prime, T., Bauw, G., Posada, P., Rouze, P., Dehais, P., Sahnoun, I., Barlier, I., and Rossingol, M., Use of a proteome strategy for tagging proteins present at the plasma membrane. Plant J., 16, 633-641 (1998).
10. Thiellement, H., Bahrman, N., Damerval, C., Plomion, C., Rossignol, M., Santoni, V., de Vienne, D., and Zivy, M., Proteomics for genetic and physiological studies in plants. Electrophoresis, 20, 2013-2026 (1999).
11. Komatsu, S., Rakwal, R., and Li Z., Separation and characterization of proteins in rice (Oryza sativa) suspension cultured cells. Plant Cell Tissue Org. Cul., 55, 183-192 (1999).
12. Peltier, J. B., Firso, G., Kalume, D. E., Roepstoff, P., Nilson, F., Adamska, I., and van Wijl, K. J., Proteomics of the chroloplast: Systematic identification and targeting analysis of lumenal and peripheral thylakoid proteins. Plant Cell, 12, 319-342 (2000).
13. Mikami, S., Hori, H., and Mitsui, T., Separation of distinct compartments of rice Golgi complex by sucrose density gradient centrifugation. Plant Sci., 161, 665-675 (2001).
14. Hurkman, W. J. and Tanaka, C. K., Solubilization of plant membrane proteins for analysis by twodimensional gel electrophoresis. Plant Physiol., 81, 802-806 (1986).
15. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
16. Celis, J. E., Rasmussen, H. H., Olsen, E., Madsen, P., Leffers, H., Honnore, B., Dejgaard, K., Gromov, P., Hoffmann, H. J., Nielsen, M., Vassilev, A., Vintermyr, O., Hao, J., Celis, A., Basse, B., Lauridsen, J. B., Ratz, G. P., Andersen, A. H., Kjaergaard, E., Puype, M., Damme, J. V., and Vandekerckhove, J., The human keratinocyte twodimensional gel protein database: Update 1993. Electrophoresis, 14, 1091-1198 (1993).