PCR detection and prevalence of enterotoxin (cpe) gene in Clostridium perfringens isolated from diarrhea patients

Journal of the Medical Association of Thailand

Volumn 85, Issue 5, 2002, Pages 624-632

  Tansuphasiri, Unchalee ^a   Wongsuvan, Gumphol ^a   Eampokalap, Boonchuay ^b  

^a MAHIDOL UNIVERSITY   (Thailand)

^b Bamrasnaradura Hospital   (Thailand)

Author keywords

Clostridium Perfringens; Diarrhea Patients; Duplex PCR; Enterotoxin

Indexed keywords

ENTEROTOXIN; ENTEROTOXIN, CLOSTRIDIUM;

ADOLESCENT; ADULT; AGED; ARTICLE; CHILD; CLOSTRIDIUM PERFRINGENS; DIARRHEA; FECES; FEMALE; HUMAN; MALE; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD; CHILD, PRESCHOOL; CLOSTRIDIUM PERFRINGENS; DIARRHEA; ENTEROTOXINS; FECES; FEMALE; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION;

EID: 0036559713     PISSN: 01252208     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Bean NH, Griffin PM. Foodborne disease outbreaks in the United States, 1973-1987; pathogens, vehicles and trends. J Food Prot 1990; 53: 804-17.
2. McClane BA. Clostridium perfringens enterotoxin: structure, action and detection. J Fd Saf 1992; 12: 237-52.
3. Duncan CL, Strong DH, Sebald M. Sporulation and enterotoxin production by mutants of Clostridium perfringens. J Bacteriol 1972; 110: 378-91.
4. McDonel JL. Clostridium perfringens toxins (type A, B, C, D, E). Pharmacol Ther 1980; 10: 617-55.
5. Birkhead G, Vogt RL, Heun EM, Snyder JT, McClane BA. Characterization of an outbreak of Clostridium perfringens food poisoning by quantitative fecal culture and fecal enterotoxin measurement. J Clin Microbiol 1988; 26: 471-4.
6. Borriello SP, Larson HE, Welch AR, et al. Enterotoxigenic Clostridium perfringens: A possible cause of antibiotic-associated diarrhoea. Lancet 1984; i: 305-7.
7. Mpamugo O, Donovan T, Brett MM. Enterotoxigenic Clostridium perfringens as a cause of sporadic cases of diarrhoea. J Med Microbiol 1995; 43: 442-5.
8. Kokai-Kun JF, Songer JG, Czeczulin JR, Chen F, McClane BA. Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens. J Clin Microbiol 1994; 32: 2533-9.
9. Van Damme-Jongsten M, Wernars K, Notermans S. Cloning and sequencing of the Clostridium perfringens enterotoxin gene. Antonie van Leeuwenhoek 1989; 56: 181-90.
10. Bartholomew BA, Stringer MF, Watson GN, Gilbert RJ. Development and application of an enzyme-linked immunosorbent assay for Clostridium perfringens type A enterotoxin. J Clin Pathol 1985; 38: 222-8.
11. McClane BA, Strouse RJ. Rapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay. J Clin Microbiol 1984; 19: 112-5.
12. Berry PR, Rodhouse JC, Hughes S, Batholomew BA, Gilbert RJ. Evaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens. J Clin Pathol 1988; 41: 458-61.
13. Uemura T, Yoshitake S, Hu D, Kajikawa T. A highly sensitive enzyme-linked immunosorbent assay for Clostridium perfringens enterotoxin. Lett Appl Microbiol 1992; 15: 23-5.
14. Hill WE. The polymerase chain reaction: Applications for the detection of foodborne pathogens. Crit Rev Food Sci Nutr 1996; 36: 123-73.
15. Fach P, Guillou JP. Detection by in vitro amplification of the alpha-toxin (phospholipase C) gene from Clostridium perfringens. J Appl Bacteriol 1993; 74: 61-6.
16. Fach P, Popoff MR. Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test. Appl Environ Microbiol 1997; 63: 4232-6.
17. Ridell J, Bjcrkroth J, Eisgruber H, Schalch B, Stolle A, Korkeala H. Prevalence of the enterotoxin gene and clonality of Clostridium perfringens strains associated with food-poisoning outbreaks. J Food Protect 1998; 61: 240-3.
18. Saito M, Matsumoto M, Funabashi M. Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure. Int J Food Microbiol 1992; 17: 47-55.
19. Tansuphasiri U. Development of duplex PCR assay for rapid detection of enterotoxigenic isolates of Clostridium perfringens. Southeast Asian J Trop Med Public Health 2001; 32: 105-13.
20. Harmon SM, Kautter DA, Hatheway CL. Enumeration and characterization of Clostridium perfringens in patient and normal controls. J Food Prot 1986; 49 706-11.
21. Cato EP, George WL, Finegold SM. Genus Clostridium. In : Sneath P, ed. Bergey's manual of systemic bacteriology. vol. 2. Baltimore, Md. : Williams and Wilkins, 1986: 1141-82.
22. Kwok S, Higuchi R. Avoiding false positive with PCR. Nature (London) 1989; 339: 237-8.
23. Division of Epidemiology, Office of the Permanent Secretary for Public Health, Ministry of Public Health, Annual epidemiological surveillance report, Thailand, 1999: 55-66.
24. McClane BA. Clostridium perfringens. In : Doyle, MP, Beuchat LR, Montville TJ, eds. Food microbiology: fundamentals and frontiers. Washington DC: ASM Press, 1997: 305-26.
25. Wada A, Masuda Y, Fukayama M, et al. Nosocomial diarrhoea in the elderly due to enterotoxigenic Clostridium perfringens. Microbiol Immunobiol 1996; 40: 767-71.
26. Regan CM, Syed Q, Tunstall PJ. A hospital outbreak of Clostridium perfringens food poisoning implications for food hygiene review in hospitals. J Hosp Infect 1995; 29: 69-73.
27. Murell WG, Stewart BJ, Siarakas S, Kariks S. Enterotoxigenic bacteria in the sudden infant death syndrome. J Med Microbiol 1993; 39: 114-27.
28. Lindsay J, Mach A, Wilkinson M, et al. Clostridium perfringens type A cytotoxic-enterotoxin(s) as triggers for death in sudden infant death syndrome: Development of a toxico-infection hypothesis. Curr Microbiol 1993; 27: 51-9.
29. American Public Health Association. Clostridium perfringens food poisoning. In : Benenson AS, ed. Control of communicable diseases in man. Washington, DC: American Public Health Association, 1985: 148-9.
30. Stringer MF, Watson GN, Gilbert RJ, et al. Faecal carriage of Clostridium perfringens. J Hyg 1985; 95: 277-88.
31. Yamagishi T, Serikawa T, Morita R, Nakamura S, Nishida S. Persistent high numbers of Clostridium perfringens in the intestines of Japanese aged adults. Jpn J Microbiol 1976; 20: 397-403.
32. Melville SB, Collie RE, McClane BA. Regulation of enterotoxin production in Clostridium perfringens. In : Rood J, Songer G, McClane BA, Tiball R, eds. Molecular biology and pathogenesis of the Clostridia. London : Academic Press, 1997: 471-87.
33. MacFarlane MG, Knight BCJG. The biochemistry of bacterial toxins. I The lecithinase activity of Cl. welchii toxins. Biochem J 1941; 35: 884-902.
34. Titball RW, Hunter SEC, Martin KL, et al. Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens. Infect Immun 1989; 57: 367-76.
35. Czeczulin JR, Hanna PC, McClane BA. Cloning, nucleotide sequencing, and expression of the Clostridium perfringens enterotoxin gene in Escherichia coli. Infect Immun 1993; 61: 3429-39.
36. Collie RE, McClane BA. Evidence that the enterotoxin gene can be episomal in Clostridium perfringens isolates associated with nonfoodborne human gastrointestinal diseases. J Clin Microbiol 1998; 36: 30-6.
37. Brynestad S, Sarker MR, McClane BA, Granum PE, Rood JI. Enterotoxin plasmid from Clostridium perfringens is conjugative. Infect Immun 2001; 69: 3483-7.