A small-volume technique for simultaneous immunophenotyping and apoptosis detection in rat whole blood by four-color flow cytometry

Cytometry

Volumn 47, Issue 4, 2002, Pages 265-275

  Diaz Romero, Jose ^a   Vogt, Gerard ^a   Weckbecker, Gisbert ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Apoptosis; Dexamethasone; Flow cytometry; Immunophenotyping; Permeabilization; Rat; TO PRO 3; Whole blood

Indexed keywords

CD3 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; CELL DNA; CELL SURFACE MARKER; DEXAMETHASONE; DYE; BIOLOGICAL MARKER; CARBOCYANINE; DETERGENT; DIAGNOSTIC AGENT; DNA; FLUORESCENT DYE; MEMBRANE ANTIGEN; TO PRO 3; TO-PRO-3;

ANIMAL TISSUE; ANTIGEN DETECTION; APOPTOSIS; ARTICLE; BLOOD; CELL PERMEABILIZATION; CONTROLLED STUDY; DNA CONTENT; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; IMMUNOFLUORESCENCE; IMMUNOMAGNETIC SEPARATION; IMMUNOPHENOTYPING; INCUBATION TIME; LYMPHOCYTE; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; BLOOD CELL; CELL MEMBRANE PERMEABILITY; CYTOLOGY; DRUG EFFECT; GENETICS; IMMUNOLOGY; INSTRUMENTATION; LEWIS RAT; MALE; METHODOLOGY; PHYSIOLOGY; T LYMPHOCYTE;

ANIMALIA;

ANIMALS; ANTIGENS, CD3; ANTIGENS, CD4; ANTIGENS, CD8; ANTIGENS, SURFACE; APOPTOSIS; BIOLOGICAL MARKERS; BLOOD CELLS; CARBOCYANINES; CELL MEMBRANE PERMEABILITY; DETERGENTS; DNA; FLOW CYTOMETRY; FLUORESCENT DYES; IMMUNOPHENOTYPING; MALE; RATS; RATS, INBRED LEW; T-LYMPHOCYTES;

EID: 0036525919     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.10081     Document Type: Article

References (28)

1. Rapid single-step method for flow cytometric detection of surface and intracellular antigens using whole blood
2. Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats
3. Evolution of leukocyte immunophenotyping as influenced by the HIV/AIDS pandemic: A short history of the development of gating strategies for CD4+ T-cell enumeration
4. Evaluation of red blood cell lysing solutions for the detection of intracellular antigens by flow cytometry
5. Analysis of peripheral blood mononuclear cells in rodents by three-colour flow cytometry using a small-volume lysed whole blood technique
6. Blood leucocyte subsets of the rat: Expression of adhesion molecules and localization within high endothelial venules
7. Effect of red cell lysis protocols on the expression of rat peripheral blood lymphocyte subset and activation antigens
8. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
9. Refinement of blood sampling from the sublingual vein of rats
10. Apoptosis induction by the glucocorticoid hormone dexamethasone and the calcium-ATPase inhibitor thapsigargin involves Bcl-2 regulated caspase activation
11. Investigation of glucocorticoid-induced apoptotic pathway: Processing of caspase-6 but not caspase-3
12. Dexamethasone and interleukins modulate apoptosis of murine thymocytes and peripheral T-lymphocytes
13. Identification of nuclei from apoptotic, necrotic, and viable lymphoid cells by using multiparameter flow cytometry
14. Comparison of four lymphocyte isolation methods applied to rodent T cell subpopulations and B cells
15. TO-PRO-3 iodide: A novel HeNe laser-excitable DNA stain as an alternative for propidium iodide in multiparameter flow cytometry
16. Multiparameter flow cytometry as a tool to evaluate antipsoriatic therapy
17. Hematologic values of New Zealand white rabbits determined by automated flow cytometry
18. Cytometry, in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis)
19. Apoptosis, graft rejection, and transplantation tolerance
20. Requirement for T-cell apoptosis in the induction of peripheral transplantation tolerance
21. Blocking both signal 1 and signal 2 of T-cell activation prevents apoptosis of alloreactive T cells and induction of peripheral allograft tolerance
22. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods
23. The study of apoptotic cells by flow cytometry
24. A simplified method for the coordinate examination of apoptosis and surface phenotype of murine lymphocytes
25. Detection of apoptosis of immature CD4+8+ thymocytes by flow cytometry
26. A simple fluorescence method for surface antigen phenotyping of lymphocytes undergoing DNA fragmentation
27. A cytofluorometric method for the simultaneous detection of both intracellular and surface antigens of apoptotic peripheral lymphocytes
28. Spontaneous and glucocorticoid-induced apoptosis in human mature T lymphocytes