Site-specific tryptophan fluorescence spectroscopy as a probe of membrane peptide structure and dynamics

European Biophysics Journal

Volumn 31, Issue 1, 2002, Pages 9-13

  Clayton, Andrew H ^a,b   Sawyer, William H ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Amphipathic helix; Dynamics; Lipid protein interactions; Time resolved fluorescence spectroscopy; Tryptophan

Indexed keywords

PEPTIDE; TRYPTOPHAN;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; CONFERENCE PAPER; DYNAMICS; FLUORESCENCE SPECTROSCOPY; LIPID BILAYER; MODEL; PEPTIDE ANALYSIS; PROTEIN CONFORMATION; PROTEIN LIPID INTERACTION; PROTEIN STRUCTURE;

BIOPHYSICS; FLUORESCENCE POLARIZATION; MEMBRANE PROTEINS; MOLECULAR STRUCTURE; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; TRYPTOPHAN;

EID: 0036519842     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490100182     Document Type: Conference Paper

References (21)

1. Alcala JR (1995) The effect of harmonic conformational trajectories on protein fluorescence and lifetime distributions. J Chem Phys 101: 4578-4584
2. Alcala JR, Gratton E, Prendergast FG (1987) Fluorescence lifetime distributions in proteins. Biophys J 51:597-604
3. Chen RF, Knutson JR, Ziffer H, Porter D (1991) Fluorescence of tryptophan dipeptides: correlations with the rotamer model. Biochemistry 30:5184-5195
4. Chung LA, Lear JD, De Grado WF (1992) Fluorescence studies of the secondary structure and orientation of a model ion channel peptide in phospholipid vesicles. Biochemistry 31: 6608-6616
5. Clayton AHA, Sawyer WH (1999a) The structure and orientation of class A amphipathic peptides on a phospholipid bilayer surface. Eur Biophys J 28:133-141
6. Clayton AHA, Sawyer WH (1999b) Tryptophan rotamer distributions in amphipathic peptides at a lipid surface. Biophys J 76:3235-3242
7. Clayton AHA, Sawyer WH (2000) Site-specific tryptophan dynamics in class A amphipathic helical peptides at a phospholipid bilayer interface. Biophys J 79:1066-1073
8. Hristova K, Wimley WC, Mishra VK, Antharamiah GM, Segrest JP, White SH. (1999) An amphipathic α-helix at a membrane interface: a structural study using a novel X-ray diffraction method. J Mol Biol 290:99-117
9. Hudson BS, Huston JM, Soto-Campos G (1999) A reversible dark state mechanism for the complexity of the fluorescence of tryptophan in proteins. J Phys Chem A 103: 2227-2234
10. Ladokhin AS (1999a) Evaluation of lipid exposure of tryptophan residues in membrane proteins and peptides. Anal Biochem 276:65-71
11. Ladokhin AS (1999b) Red-edge excitation study of non-exponential fluorescence decay of indole in solution and in a protein. J Fluores 9:1-9
12. Maiorano JN, Davidson WS (2000) The orientation of helix 4 in apolipoprotein A-I-containing reconstituted high density lipoproteins. J Biol Chem 275:17374-17380
13. Millar DP (1996) Time-resolved fluorescence. Curr Opin Struct Biol 6:637-642
14. O'Neil KT, Wolfe HR, Erickson-Viitanen S, Degrado WF (1987) Fluorescence properties of calmodulin-binding peptides reflect alpha-helical periodicity. Science 236:1454-1456
15. Ren J, Lew S, Wang Z, London E (1997) Transmembrane orientation of hydrophobic-helices is regulated both by the relationship of helix length to bilayer thickness and by the cholesterol concentration. Biochemistry 36:10213-10220
16. Sillen A, Fernando Diaz J, Engelborghs Y (2000) A step toward the prediction of the fluorescence lifetimes of tryptophan residues in proteins based on structural and spectral data. Protein Sci 9:158-169
17. Soulages JL, Arrese EL (2000) Fluorescence spectroscopy of single tryptophan mutants of apolipophorin-III in discoidal lipoproteins of dimyristoylphosphatidylcholine. Biochemistry 39:10574-10580
18. Szabo AG, Rayner DM (1980) Fluorescence decay of tryptophan conformers in aqueous solution, J Am Chem Soc 102:554-563
19. Vogel H, Nilsson L, Rigler R, Voges K-P, Jung G (1988) Structural fluctuations of a helical polypeptide traversing a lipid bilayer. Proc Natl Acad Sci USA 85:5067-5071
20. Voges KP, Jung G, Sawyer WH (1987) Depth-dependant fluorescence quenching of a tryptophan residue located at defined positions on a rigid 21-peptide helix in liposomes. Biochim Biophys Acta 896:64-76
21. Wimley WC, White SH (2000) Determining the membrane topology of peptides by fluorescence quenching. Biochemistry 39:161-170