Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula

Photochemistry and Photobiology

Volumn 75, Issue 3, 2002, Pages 311-315

  Morishita, Hisashi ^a   Ohashi, Sayumi ^a   Oku, Takashi ^b   Nakajima, Yoshihiro ^b   Kojima, Satoshi ^b   Ryufuku, Masayuki ^a   Nakamura, Hideshi ^c   Ohmiya, Yoshihiro ^a,b  

^a SHIZUOKA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; HISTIDINE; LUCIFERASE; LUCIFERIN; OXYGEN; PYRROLE DERIVATIVE; RECOMBINANT HISTIDINE TAGGED LUCIFERASE; UNCLASSIFIED DRUG; PRIMER DNA;

AFFINITY CHROMATOGRAPHY; ALGA; AMINO ACID SEQUENCE; ARTICLE; BIOLUMINESCENCE; DINOFLAGELLATE; ENZYME ANALYSIS; ENZYME STRUCTURE; EXTRACTION; LINGULODINIUM POLYEDRUM; LUMINESCENCE; MARINE ENVIRONMENT; MOLECULAR CLONING; NONHUMAN; OXIDATION; PH MEASUREMENT; PROTEIN DOMAIN; PROTEIN PURIFICATION; PYROCYSTIC LUNULA; SEQUENCE HOMOLOGY; ANIMAL; BIOLOGY; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

ALGAE; DINOPHYCEAE; LINGULODINIUM; LINGULODINIUM POLYEDRUM; PYROCYSTIS; PYROCYSTIS LUNULA;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DINOFLAGELLIDA; DNA PRIMERS; DNA, COMPLEMENTARY; LUCIFERASES; LUMINESCENCE; MARINE BIOLOGY; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0036517606     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1562/0031-8655(2002)075<0311:CACOAA>2.0.CO;2     Document Type: Article

References (17)

1. Fogel, M. and J. W. Hastings (1971) A substrate-binding protein in the Gonyaulax bioluminescence reaction. Arch. Biochem. Biophys. 142, 310-321.
2. Hastings, J. W. (1978) In Bioluminescence in Action (Edited by P. J. Herring), pp. 129-170. Academic Press, London.
3. Hastings, J. W. (1993) Dinoflagellates: cell biochemistry and its regulation of the millisecond and 24 hour time scales. Nav. Res. Rev. XLV, 21-30.
4. Dunlap, J. C. and J. W. Hastings (1981) Biochemistry of dinoflagellate bioluminescence: purification and characterization of dinoflagellate luciferin from Pyrocystis lunula. Biochemistry 20, 983-989.
5. Rosemarie, K., T. Urbig, L. Li, W. Taylor and J. W. Hastings (1998) The circadian rhythm of bioluminescence in Pyrocystis is not due to differences in the amount of luciferase: a comparative study of three bioluminescent marine dinoflagellates. J. Phycol. 34. 167-172.
6. Schmitter, R. E., D. Njus, F. M. Sulzman, V. D. Gooch and J. W. Hastings (1976) Dinoflagellate bioluminescence: a comparative study of in vitro components. J. Cell. Physiol. 87, 123-134.
7. Bae, Y. M. and J. W. Hastings (1994) Cloning, sequencing and expression of dinoflagellate luciferase DNA from a marine alga, Lingulodinium polyedrum. Biochem. Biophys. Acta 1219, 449-456.
8. Li, L., R. Hong and J. W. Hastings (1997) Three functional luciferase domains in a single polypeptide chain. Proc. Natl. Acad. Sci. USA 94, 8954-8958.
9. Krieger, N. and J. W. Hastings (1968) Bioluminescence: pH activity profiles of related luciferase fractions. Science 161, 586-589.
10. Krieger, N., D. Njus and J. W. Hastings (1974) An active proteolytic fragment of Gonyaulax luciferase. Biochemistry 13, 2871-2877.
11. Li, L., L. Liu, R. Hong, D. Robertson and J. W. Hastings (2001) N-terminal intramolecularly conserved histidines of three domains in Gonyaulax luciferase are responsible for loss of activity in the alkaline region. Biochemistry 40, 1844-1849.
12. Okamoto, O. K., L. Liu, D. L. Robertson and J. W. Hastings (2001) Molecular structure of the luciferase gene from the dinoflagellate Pyrocystis lunula. In Program and Abstracts at the 29th Annual Meeting of the American Society for Photobiology. Chicago, Illinois, July 7-12, 2001.
13. Lecuyer, B., B. Arrio, C. Fresneau and P. Volfin (1979) Dinoflagellate luciferase: purification of luciferase from Lingulodinium polyedrum, Pyrocystis lunula and Pyrocystis fusiformis. Arch. Biochem. Biophys. 196, 371-384.
14. Guillard, R. R. L. and J. H. Ryther (1962) Studies on marine planktonic diatoms. I. Qyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can. J. Microbiol. 8, 229-239.
15. Colepicolo, P., T. Roenneberg, D. Morse, W. R. Taylor and J. W. Hastings (1993) Circadian regulation of bioluminescence in the dinoflagellate Pyrocystis lunula. J. Phycology 29, 173-179.
16. Chomczynsky, P. and N. Sacchi (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
17. Nakamura, H., Y. Kishi, O. Shimomura, D. Morse and J. W. Hastings (1989) Structure of dinoflagellate luciferin and its enzymatic air-oxidation products. J. Am. Chem. Soc. 111, 7607-7611.