A colorimetric assay for the quantitation of free adenine applied to determine the enzymatic activity of ribosome-inactivating proteins

Analytical Biochemistry

Volumn 302, Issue 1, 2002, Pages 114-122

  Heisler, Iring ^a   Keller, Jutta ^a   Tauber, Rudolf ^a   Sutherland, Mark ^a   Fuchs, Hendrik ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Adenine phosphoribosyl transferase; Adenine quantitation; Adenine release assay; Dianthin; Immunotoxins; Ribosome inactivating protein; Ricin; Saporin

Indexed keywords

COLOR; COLORIMETRY; MACROMOLECULES; PLANTS (BOTANY); SUBSTRATES;

ADENINE QUANTITATION; DIANTHIN; IMMUNOTOXINS; PHOSPHORIBOSYL TRANSFERASE; RIBOSOME-INACTIVATING PROTEIN; RICIN; SAPORIN;

PROTEINS;

ADENINE; ADENINE PHOSPHORIBOSYLTRANSFERASE; ADENOSINE PHOSPHATE; IMMUNOTOXIN; PHOSPHATE; PURINE DERIVATIVE; RIBOSOME INACTIVATING PROTEIN; RICIN; SAPORIN;

ANALYTIC METHOD; ARTICLE; COLORIMETRY; DEPHOSPHORYLATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN TRANSPORT; QUANTITATIVE ASSAY; RADIOACTIVITY; TECHNIQUE;

EID: 0036499268     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5527     Document Type: Article

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