Simultaneous detection of two verotoxin genes using dual-label time-resolved fluorescence immunoassay with duplex PCR

Luminescence

Volumn 17, Issue 2, 2002, Pages 123-129

  Watanabe, Kazuyuki ^a,b   Arakawa, Hidetoshi ^a   Maeda, Masako ^a  

^a SHOWA UNIVERSITY   (Japan)

^b SHOWA UNIVERSITY HOSPITAL   (Japan)

Author keywords

Duplex PCR; Enterohaemorrhagic Escherichia coli (EHEC); Time resolved fluorescence immunoassay (TRFIA); Verotoxin gene

Indexed keywords

ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; DISEASES; ESCHERICHIA COLI; FLUORESCENCE;

DUAL LABELS; DUPLEX PCR; ENTEROHEMORRHAGIC ESCHERICHIA COLI; HAEMORRHAGIC COLITIS; SEROTYPES; SIMULTANEOUS DETECTION; TIME-RESOLVED FLUORESCENCE IMMUNOASSAY; TIME-RESOLVED FLUORESCENCE IMMUNOASSAYS; VEROTOXIN GENE;

GENES;

ESCHERICHIA COLI;

AVIDIN; BIOTIN; DIAGNOSTIC AGENT; LANTHANIDE; SHIGA TOXIN; VIRUS DNA;

ARTICLE; ENTEROBACTER INFECTION; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOASSAY; METHODOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY;

AVIDIN; BIOTIN; DNA, VIRAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; HUMANS; IMMUNOASSAY; LANTHANOID SERIES ELEMENTS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXINS; SPECTROMETRY, FLUORESCENCE;

EID: 0036491742     PISSN: 15227235     EISSN: None     Source Type: Journal    
DOI: 10.1002/bio.673     Document Type: Article

References (13)

1. Takeda Y, Kurazono H and Yamasaki S. Vero-toxins produced by enterohemorrhagic Escherichia coli (verotoxin-producing E. coli). Microbiol. Immunol. 1993; 37: 591-599.
2. Ramotar K, Waldhart B, Church D, Szumski R and Louie TJ. Direct detection of verotoxin-producing Escherichia coli in stool sample by PCR. J. Clin. Microbiol. 1995; 33: 519-524.
3. Makristathis A, Fasching E, Schutze K, Wimmer M, Rotter ML and Hirschl AM. Detection of Helicobacter pylori in stool specimens by PCR and antigen enzyme immunoassay. J. Clin. Microbiol. 1998; 36: 2772-2774.
4. Sander A and Penno S. Semiquantitative species-specific detection of Bartonella henselae and Bartonella quinitana by PCR-enzyme immunoassay. J. Clin. Microbiol. 1999; 37: 3097-3101.
5. Arakawa H, Nakasiro S, Tsuji A and Maeda M. Analysis of polymerase chain reaction products by high performance liquid chromatography with fluorimetric detection and its application to DNA diagnosis. J. Chromatog. A 1998; 787: 261-265.
6. Arakawa H, Uetenaka K, Maeda M and Tsuji A. Analysis of polymerase chain reaction product by capillary electrophoresis and its application to the detection of single base substitution in genes. J. Chromatog. A 1994; 664: 89-98.
7. D'Amato RF, Wallman AA and Hochstein LH et al. Rapid diagnosis of pulmonary tuberculosis by using Roche Amplicor Mycobacterium tuberculosis PCR test. J. Clin. Microbiol. 1995; 33: 1832-1834.
8. Soini E and Kojola H. Time-resolved fluorometer for lanthanide chelates - new generation of nonisotopic immunoassay. Clin. Chem. 1983; 29: 65-68.
9. Watanabe K, Oda M, Arakawa H and Maede M. Simultaneous analysis of two-site polymorphisms using time-resolved fluorescence immunoassay. Anal. Sci. 2000; 16: 765-769.
10. Takao T, Tanabe T, Hong YM, Shimonishi Y, Kurazono H and Yutsudo T. Identity of molecular structure of shiga-like toxin I (VT1) from Escherichia coli O157: H7 with that of shiga toxin. Microb. Pathog. 1988; 5: 357-369.
11. Wahiberg J, Lundeberg J, Hultman T and Uhlen M. General colorimetric method for DNA diagnostics allowing direct solid-phase genomic sequencing of the positive samples. Proc. Natl. Acad. Sci. USA 1990; 87: 6569-6573.
12. Heid CA, Stevens J, Livak KJ and Williams PM. Real time quantitative PCR. Genome Res. 1996; 6: 986-994.
13. Wittwer CT, Herrmann MG, Moss AA and Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997; 22: 130-138.